Desulfobacter postgatei 2ac9

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfobacter; Desulfobacter postgatei

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3396 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I5B4K9|I5B4K9_9DELT CRISPR_Cas6 domain-containing protein OS=Desulfobacter postgatei 2ac9 OX=879212 GN=DespoDRAFT_02577 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 5.37NNLNPIPGALVEE14 pKa = 4.37GHH16 pKa = 5.85WSGLVSGNVSGTTSEE31 pKa = 3.81NGMIEE36 pKa = 4.31WVSAATIQSGTITFTVDD53 pKa = 3.46TISASGYY60 pKa = 10.89DD61 pKa = 4.01YY62 pKa = 11.25DD63 pKa = 4.5PSLNTVSSISIKK75 pKa = 7.77TQEE78 pKa = 4.54SSNQSPVAVIDD89 pKa = 3.88GGPVSGMEE97 pKa = 3.75GDD99 pKa = 3.87SIYY102 pKa = 10.97FEE104 pKa = 4.95GKK106 pKa = 9.89NSYY109 pKa = 10.93DD110 pKa = 3.52PDD112 pKa = 5.62AGDD115 pKa = 3.38TLSYY119 pKa = 8.52EE120 pKa = 3.56WRR122 pKa = 11.84MGEE125 pKa = 4.3EE126 pKa = 3.62ILSYY130 pKa = 9.38GTYY133 pKa = 10.35LIHH136 pKa = 6.9EE137 pKa = 5.14FSTAGTYY144 pKa = 9.26EE145 pKa = 3.98VILRR149 pKa = 11.84VEE151 pKa = 4.66DD152 pKa = 3.22DD153 pKa = 3.12WGAVSEE159 pKa = 4.73DD160 pKa = 3.57KK161 pKa = 10.81VTVTIEE167 pKa = 4.0AEE169 pKa = 4.09SGNDD173 pKa = 3.25AFIHH177 pKa = 5.5VSSIEE182 pKa = 3.94MQVSYY187 pKa = 10.97LGRR190 pKa = 11.84NSLGLATVKK199 pKa = 10.59VVDD202 pKa = 3.76QYY204 pKa = 12.25GNPIEE209 pKa = 4.16NAAIKK214 pKa = 9.6GVWSGVVTASQSGITAGDD232 pKa = 3.68GTVMFTSPKK241 pKa = 8.93TKK243 pKa = 9.89TSGEE247 pKa = 4.24FVFSVDD253 pKa = 3.07GVTASGYY260 pKa = 10.63VYY262 pKa = 10.95SPDD265 pKa = 4.62DD266 pKa = 3.68NVLEE270 pKa = 4.6LNSINNN276 pKa = 3.64

Molecular weight: 29.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I5B510|I5B510_9DELT dTDP-4-dehydrorhamnose 3 5-epimerase OS=Desulfobacter postgatei 2ac9 OX=879212 GN=DespoDRAFT_02738 PE=3 SV=1
MM1 pKa = 7.59IFLGSVIKK9 pKa = 10.43KK10 pKa = 9.31RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.28KK14 pKa = 9.1MRR16 pKa = 11.84KK17 pKa = 7.92HH18 pKa = 4.98KK19 pKa = 10.0HH20 pKa = 5.3RR21 pKa = 11.84KK22 pKa = 8.54LLAKK26 pKa = 9.59TRR28 pKa = 11.84HH29 pKa = 4.37QRR31 pKa = 11.84KK32 pKa = 9.36KK33 pKa = 10.32KK34 pKa = 10.13

Molecular weight: 4.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3396

0

3396

1120179

29

3812

329.9

36.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.051 ± 0.04

1.312 ± 0.016

5.787 ± 0.032

6.168 ± 0.037

4.558 ± 0.027

7.066 ± 0.037

2.093 ± 0.018

7.303 ± 0.039

6.467 ± 0.04

9.635 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 0.02

4.062 ± 0.026

4.292 ± 0.026

3.637 ± 0.027

5.053 ± 0.033

5.926 ± 0.031

5.398 ± 0.028

6.45 ± 0.032

1.021 ± 0.015

2.948 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski