Torque teno virus 1 (isolate TA278)
Average proteome isoelectric point is 7.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|O70739|CAPSD_TTVA1 Capsid protein OS=Torque teno virus 1 (isolate TA278) OX=766182 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.82 AEE3 pKa = 3.98 FSTPVRR9 pKa = 11.84 SGEE12 pKa = 3.93 ATEE15 pKa = 4.44 GDD17 pKa = 3.76 LRR19 pKa = 11.84 VPRR22 pKa = 11.84 AGAEE26 pKa = 4.13 GEE28 pKa = 4.28 FTHH31 pKa = 7.38 RR32 pKa = 11.84 SQGAIRR38 pKa = 11.84 ARR40 pKa = 11.84 DD41 pKa = 3.39 WPGYY45 pKa = 8.67 GQGSEE50 pKa = 3.41 KK51 pKa = 11.13 SMFIGRR57 pKa = 11.84 HH58 pKa = 3.85 YY59 pKa = 10.34 RR60 pKa = 11.84 KK61 pKa = 9.85 KK62 pKa = 10.47 RR63 pKa = 11.84 ALSLCAVRR71 pKa = 11.84 TTKK74 pKa = 10.42 KK75 pKa = 10.25 ACKK78 pKa = 9.62 LLIVMWTPPRR88 pKa = 11.84 NDD90 pKa = 2.85 QHH92 pKa = 6.34 YY93 pKa = 10.83 LNWQWYY99 pKa = 8.46 SSILSSHH106 pKa = 6.8 AAMCGCPDD114 pKa = 3.41 AVAHH118 pKa = 6.17 FNHH121 pKa = 6.91 LASVLRR127 pKa = 11.84 APQNPPPPGPQRR139 pKa = 11.84 NLPLRR144 pKa = 11.84 RR145 pKa = 11.84 LPALPAAPEE154 pKa = 4.25 APGDD158 pKa = 3.71 RR159 pKa = 11.84 APWPMAGGAEE169 pKa = 4.36 GEE171 pKa = 4.4 DD172 pKa = 3.77 GGAGGDD178 pKa = 3.77 ADD180 pKa = 3.99 HH181 pKa = 7.08 GGAAGGPEE189 pKa = 4.48 DD190 pKa = 5.9 ADD192 pKa = 4.23 LLDD195 pKa = 4.11 AVAAAEE201 pKa = 4.19 TT202 pKa = 3.88
Molecular weight: 21.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.6
IPC2_protein 6.504
IPC_protein 6.751
Toseland 6.795
ProMoST 7.029
Dawson 7.22
Bjellqvist 7.059
Wikipedia 7.19
Rodwell 7.205
Grimsley 6.898
Solomon 7.278
Lehninger 7.293
Nozaki 7.41
DTASelect 7.512
Thurlkill 7.541
EMBOSS 7.585
Sillero 7.629
Patrickios 4.469
IPC_peptide 7.293
IPC2_peptide 7.263
IPC2.peptide.svr19 7.086
Protein with the highest isoelectric point:
>sp|O70739|CAPSD_TTVA1 Capsid protein OS=Torque teno virus 1 (isolate TA278) OX=766182 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.9 AYY3 pKa = 9.87 GWWRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 WRR14 pKa = 11.84 RR15 pKa = 11.84 WRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 PWRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 WRR26 pKa = 11.84 TRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 PARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 GRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 NVRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 GGRR50 pKa = 11.84 WRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 9.81 RR56 pKa = 11.84 RR57 pKa = 11.84 WKK59 pKa = 9.23 RR60 pKa = 11.84 KK61 pKa = 6.93 GRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 KK66 pKa = 8.81 KK67 pKa = 9.54 AKK69 pKa = 9.99 IIIRR73 pKa = 11.84 QWQPNYY79 pKa = 10.06 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 CNIVGYY88 pKa = 10.09 IPVLICGEE96 pKa = 4.13 NTVSRR101 pKa = 11.84 NYY103 pKa = 8.79 ATHH106 pKa = 6.96 SDD108 pKa = 3.57 DD109 pKa = 3.58 TNYY112 pKa = 10.32 PGPFGGGMTTDD123 pKa = 3.27 KK124 pKa = 10.02 FTLRR128 pKa = 11.84 ILYY131 pKa = 10.02 DD132 pKa = 3.34 EE133 pKa = 5.02 YY134 pKa = 11.51 KK135 pKa = 10.75 RR136 pKa = 11.84 FMNYY140 pKa = 6.42 WTASNEE146 pKa = 3.86 DD147 pKa = 3.84 LDD149 pKa = 3.9 LCRR152 pKa = 11.84 YY153 pKa = 10.15 LGVNLYY159 pKa = 9.22 FFRR162 pKa = 11.84 HH163 pKa = 6.39 PDD165 pKa = 2.59 VDD167 pKa = 5.04 FIIKK171 pKa = 9.79 INTMPPFLDD180 pKa = 3.76 TEE182 pKa = 4.24 LTAPSIHH189 pKa = 7.03 PGMLALDD196 pKa = 3.83 KK197 pKa = 10.25 RR198 pKa = 11.84 ARR200 pKa = 11.84 WIPSLKK206 pKa = 10.18 SRR208 pKa = 11.84 PGKK211 pKa = 9.5 KK212 pKa = 9.19 HH213 pKa = 5.44 YY214 pKa = 10.48 IKK216 pKa = 10.37 IRR218 pKa = 11.84 VGAPRR223 pKa = 11.84 MFTDD227 pKa = 2.77 KK228 pKa = 10.28 WYY230 pKa = 9.23 PQTDD234 pKa = 3.67 LCDD237 pKa = 3.56 MVLLTVYY244 pKa = 9.17 ATAADD249 pKa = 3.61 MQYY252 pKa = 11.06 PFGSPLTDD260 pKa = 2.83 SVVVNFQVLQSMYY273 pKa = 10.78 DD274 pKa = 3.34 KK275 pKa = 10.45 TISILPDD282 pKa = 2.98 EE283 pKa = 4.67 KK284 pKa = 10.83 SQRR287 pKa = 11.84 EE288 pKa = 3.75 ILLNKK293 pKa = 8.31 IASYY297 pKa = 10.79 IPFYY301 pKa = 10.72 NTTQTIAQLKK311 pKa = 8.87 PFIDD315 pKa = 4.21 AGNVTSGATATTWASYY331 pKa = 11.26 INTTKK336 pKa = 9.74 FTTATTTTYY345 pKa = 10.67 AYY347 pKa = 9.16 PGTNRR352 pKa = 11.84 PPVTMLTCNDD362 pKa = 2.26 SWYY365 pKa = 10.77 RR366 pKa = 11.84 GTVYY370 pKa = 9.28 NTQIQQLPIKK380 pKa = 9.89 AAKK383 pKa = 10.05 LYY385 pKa = 11.17 LEE387 pKa = 4.27 ATKK390 pKa = 9.99 TLLGNTFTNEE400 pKa = 3.96 DD401 pKa = 3.65 YY402 pKa = 10.61 TLEE405 pKa = 4.12 YY406 pKa = 10.63 HH407 pKa = 6.55 GGLYY411 pKa = 10.39 SSIWLSPGRR420 pKa = 11.84 SYY422 pKa = 11.65 FEE424 pKa = 3.86 TTGAYY429 pKa = 9.18 TDD431 pKa = 3.12 IKK433 pKa = 10.83 YY434 pKa = 10.91 NPFTDD439 pKa = 3.29 RR440 pKa = 11.84 GEE442 pKa = 4.91 GNMLWIDD449 pKa = 3.58 WLSKK453 pKa = 11.24 KK454 pKa = 10.29 NMNYY458 pKa = 10.8 DD459 pKa = 3.49 KK460 pKa = 11.15 VQSKK464 pKa = 10.38 CLISDD469 pKa = 4.25 LPLWAAAYY477 pKa = 9.96 GYY479 pKa = 10.96 VEE481 pKa = 5.33 FCAKK485 pKa = 9.33 STGDD489 pKa = 3.65 QNIHH493 pKa = 5.17 MNARR497 pKa = 11.84 LLIRR501 pKa = 11.84 SPFTDD506 pKa = 3.55 PQLLVHH512 pKa = 6.69 TDD514 pKa = 3.3 PTKK517 pKa = 11.11 GFVPYY522 pKa = 10.54 SLNFGNGKK530 pKa = 8.58 MPGGSSNVPIRR541 pKa = 11.84 MRR543 pKa = 11.84 AKK545 pKa = 8.79 WYY547 pKa = 7.13 PTLFHH552 pKa = 5.65 QQEE555 pKa = 4.27 VLEE558 pKa = 4.87 ALAQSGPFAYY568 pKa = 10.25 HH569 pKa = 6.47 SDD571 pKa = 3.2 IKK573 pKa = 10.84 KK574 pKa = 10.15 VSLGMKK580 pKa = 9.93 YY581 pKa = 9.58 RR582 pKa = 11.84 FKK584 pKa = 10.37 WIWGGNPVRR593 pKa = 11.84 QQVVRR598 pKa = 11.84 NPCKK602 pKa = 9.17 EE603 pKa = 4.1 THH605 pKa = 6.01 SSGNRR610 pKa = 11.84 VPRR613 pKa = 11.84 SLQIVDD619 pKa = 3.88 PKK621 pKa = 11.07 YY622 pKa = 10.83 NSPEE626 pKa = 4.2 LTFHH630 pKa = 5.72 TWDD633 pKa = 3.33 FRR635 pKa = 11.84 RR636 pKa = 11.84 GLFGPKK642 pKa = 9.81 AIQRR646 pKa = 11.84 MQQQPTTTDD655 pKa = 2.42 IFSAGRR661 pKa = 11.84 KK662 pKa = 8.47 RR663 pKa = 11.84 PRR665 pKa = 11.84 RR666 pKa = 11.84 DD667 pKa = 2.94 TEE669 pKa = 4.53 VYY671 pKa = 10.05 HH672 pKa = 6.82 SSQEE676 pKa = 4.27 GEE678 pKa = 4.15 QKK680 pKa = 10.66 EE681 pKa = 4.53 SLLFPPVKK689 pKa = 10.12 LLRR692 pKa = 11.84 RR693 pKa = 11.84 VPPWEE698 pKa = 4.86 DD699 pKa = 3.18 SQQEE703 pKa = 4.11 EE704 pKa = 4.7 SGSQSSEE711 pKa = 3.98 EE712 pKa = 3.99 EE713 pKa = 4.26 TQTVSQQLKK722 pKa = 9.41 QQLQQQRR729 pKa = 11.84 ILGVKK734 pKa = 9.95 LRR736 pKa = 11.84 LLFNQVQKK744 pKa = 10.59 IQQNQDD750 pKa = 2.75 INPTLLPRR758 pKa = 11.84 GGDD761 pKa = 3.42 LASLFQIAPP770 pKa = 3.53
Molecular weight: 90.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.721
IPC_protein 10.321
Toseland 10.321
ProMoST 10.058
Dawson 10.511
Bjellqvist 10.233
Wikipedia 10.716
Rodwell 10.789
Grimsley 10.599
Solomon 10.555
Lehninger 10.511
Nozaki 10.306
DTASelect 10.218
Thurlkill 10.365
EMBOSS 10.73
Sillero 10.423
Patrickios 10.335
IPC_peptide 10.555
IPC2_peptide 9.121
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
972
202
770
486.0
55.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.996 ± 3.844
1.235 ± 0.343
4.835 ± 0.281
3.807 ± 0.754
3.498 ± 0.699
7.202 ± 1.926
1.955 ± 0.695
4.527 ± 1.172
5.144 ± 1.001
7.819 ± 0.181
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.263 ± 0.098
4.218 ± 0.802
7.202 ± 1.242
5.144 ± 1.001
9.877 ± 0.9
5.761 ± 0.145
6.996 ± 1.625
4.218 ± 0.346
2.881 ± 0.187
4.424 ± 1.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here