Roseburia sp. AM59-24XD

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; unclassified Roseburia

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A416HTM2|A0A416HTM2_9FIRM ATP synthase subunit c OS=Roseburia sp. AM59-24XD OX=2293138 GN=atpE PE=3 SV=1
MM1 pKa = 7.48GLFSRR6 pKa = 11.84IFGGKK11 pKa = 9.16SIRR14 pKa = 11.84SAATSASFLGAYY26 pKa = 9.72RR27 pKa = 11.84EE28 pKa = 4.56AGGQSYY34 pKa = 11.0SGGGWGLDD42 pKa = 3.06RR43 pKa = 11.84FNSIIDD49 pKa = 3.46AHH51 pKa = 7.66SSIDD55 pKa = 4.43DD56 pKa = 4.11MIEE59 pKa = 3.61EE60 pKa = 4.38WGLADD65 pKa = 4.17EE66 pKa = 4.85GCRR69 pKa = 11.84YY70 pKa = 9.74QSLDD74 pKa = 3.63GYY76 pKa = 10.48SNPYY80 pKa = 6.68TQAYY84 pKa = 9.07RR85 pKa = 11.84EE86 pKa = 3.94EE87 pKa = 4.28RR88 pKa = 11.84EE89 pKa = 3.9RR90 pKa = 11.84AEE92 pKa = 4.16EE93 pKa = 3.95EE94 pKa = 4.5VEE96 pKa = 4.12VLAMFGEE103 pKa = 4.69EE104 pKa = 4.04IDD106 pKa = 4.21VEE108 pKa = 5.18LLIDD112 pKa = 3.55WDD114 pKa = 4.25TVEE117 pKa = 4.2EE118 pKa = 4.04NAYY121 pKa = 10.15EE122 pKa = 4.14YY123 pKa = 11.31AEE125 pKa = 4.35EE126 pKa = 5.02LAQAWLDD133 pKa = 3.34GDD135 pKa = 3.48EE136 pKa = 4.94WIPEE140 pKa = 4.42EE141 pKa = 4.12IMDD144 pKa = 4.11WAWYY148 pKa = 9.72DD149 pKa = 4.14LSDD152 pKa = 4.46HH153 pKa = 6.48NMM155 pKa = 3.34

Molecular weight: 17.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R6PHV5|A0A3R6PHV5_9FIRM Uncharacterized protein OS=Roseburia sp. AM59-24XD OX=2293138 GN=DXA20_03570 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.51KK9 pKa = 8.23KK10 pKa = 10.11SRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.42GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MRR23 pKa = 11.84TANGRR28 pKa = 11.84KK29 pKa = 9.34VILSRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84HH40 pKa = 5.5KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2844

0

2844

882024

28

3430

310.1

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.329 ± 0.054

1.502 ± 0.02

5.93 ± 0.041

7.271 ± 0.047

3.814 ± 0.032

6.945 ± 0.041

1.763 ± 0.019

7.004 ± 0.046

7.086 ± 0.044

8.534 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.133 ± 0.029

4.265 ± 0.033

3.183 ± 0.028

3.663 ± 0.03

4.704 ± 0.041

6.091 ± 0.038

5.633 ± 0.046

6.955 ± 0.041

0.931 ± 0.017

4.263 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski