Streptomyces sp. NEAU-C40

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9780 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U0S6E0|A0A4U0S6E0_9ACTN ABC transporter ATP-binding protein OS=Streptomyces sp. NEAU-C40 OX=2571141 GN=FCI23_34920 PE=4 SV=1
MM1 pKa = 7.29SAVNEE6 pKa = 4.67DD7 pKa = 4.74PNPLPPDD14 pKa = 3.56SGGGGGQSDD23 pKa = 4.1PDD25 pKa = 3.27QGKK28 pKa = 9.62DD29 pKa = 4.04LRR31 pKa = 11.84YY32 pKa = 9.79LDD34 pKa = 3.82PTVGVVWTEE43 pKa = 3.61DD44 pKa = 3.66TYY46 pKa = 11.98NSDD49 pKa = 4.28PFWATQSGSGGAPPPMADD67 pKa = 2.88SGTIKK72 pKa = 10.76VNLGSVRR79 pKa = 11.84SVEE82 pKa = 4.84LNLLSNTQQAADD94 pKa = 3.28QYY96 pKa = 9.49MGLRR100 pKa = 11.84QAVMNSTVTPYY111 pKa = 10.79YY112 pKa = 9.66WGPEE116 pKa = 4.09PPTNAQTDD124 pKa = 4.2SNPVDD129 pKa = 3.25AHH131 pKa = 6.85AGLGDD136 pKa = 4.05GGNADD141 pKa = 4.78PYY143 pKa = 11.14AGSQQQLSDD152 pKa = 3.31MGQQFGSMIDD162 pKa = 3.54PVMEE166 pKa = 4.14KK167 pKa = 10.64ALYY170 pKa = 9.08QVAGALEE177 pKa = 4.0ALGTFITTVNLTGQMYY193 pKa = 9.71GQIDD197 pKa = 3.98RR198 pKa = 11.84ACKK201 pKa = 10.18FPEE204 pKa = 4.34TPP206 pKa = 3.66

Molecular weight: 21.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U0SU38|A0A4U0SU38_9ACTN Dihydrodipicolinate synthase family protein OS=Streptomyces sp. NEAU-C40 OX=2571141 GN=FCI23_01325 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.91GRR40 pKa = 11.84AKK42 pKa = 10.73LSAA45 pKa = 3.92

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9780

0

9780

2970835

25

4657

303.8

32.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.499 ± 0.038

0.842 ± 0.008

5.747 ± 0.02

5.262 ± 0.027

2.714 ± 0.013

9.24 ± 0.025

2.381 ± 0.012

3.528 ± 0.019

2.155 ± 0.02

10.2 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.74 ± 0.01

1.986 ± 0.014

6.059 ± 0.025

3.043 ± 0.017

7.886 ± 0.029

5.497 ± 0.021

6.333 ± 0.028

8.181 ± 0.024

1.558 ± 0.011

2.15 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski