Salmonella phage celemicas

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8RQU3|A0A6G8RQU3_9CAUD Uncharacterized protein OS=Salmonella phage celemicas OX=2713289 GN=celemicas_49 PE=4 SV=1
MM1 pKa = 6.69YY2 pKa = 10.24QYY4 pKa = 10.47LTVEE8 pKa = 3.67EE9 pKa = 4.96AYY11 pKa = 10.59RR12 pKa = 11.84ITNDD16 pKa = 3.72LGICPVCDD24 pKa = 3.72GDD26 pKa = 3.88NQCVYY31 pKa = 10.95RR32 pKa = 11.84EE33 pKa = 4.56LEE35 pKa = 4.29DD36 pKa = 3.51EE37 pKa = 4.26

Molecular weight: 4.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8RQY7|A0A6G8RQY7_9CAUD Uncharacterized protein OS=Salmonella phage celemicas OX=2713289 GN=celemicas_21 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.34PNDD5 pKa = 3.45LVTWTGRR12 pKa = 11.84NGEE15 pKa = 3.98IRR17 pKa = 11.84HH18 pKa = 5.75GKK20 pKa = 9.57VISLHH25 pKa = 6.12GIYY28 pKa = 10.49ARR30 pKa = 11.84VEE32 pKa = 3.27WWRR35 pKa = 11.84AHH37 pKa = 4.87SKK39 pKa = 9.66KK40 pKa = 9.88PRR42 pKa = 11.84YY43 pKa = 8.35FTMRR47 pKa = 11.84LDD49 pKa = 3.62KK50 pKa = 11.17LIVKK54 pKa = 9.65

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13202

30

852

197.0

21.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.544 ± 0.543

1.083 ± 0.132

6.09 ± 0.226

6.893 ± 0.429

3.734 ± 0.154

7.65 ± 0.282

1.659 ± 0.162

4.969 ± 0.192

5.984 ± 0.342

7.794 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.56 ± 0.164

4.348 ± 0.242

3.886 ± 0.218

3.666 ± 0.235

5.476 ± 0.251

5.984 ± 0.281

6.348 ± 0.323

7.431 ± 0.32

1.469 ± 0.143

3.431 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski