Maize streak virus genotype A (isolate Nigeria) (MSV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Maize streak virus

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P14980|REPA_MSVN Replication-associated protein A OS=Maize streak virus genotype A (isolate Nigeria) OX=10823 GN=C1 PE=1 SV=1
MM1 pKa = 7.64ASSSSNRR8 pKa = 11.84QFSHH12 pKa = 7.0RR13 pKa = 11.84NANTFLTYY21 pKa = 9.4PKK23 pKa = 10.28CPEE26 pKa = 3.99NPEE29 pKa = 4.19IACQMIWEE37 pKa = 4.44LVVRR41 pKa = 11.84WIPKK45 pKa = 9.48YY46 pKa = 9.99ILCARR51 pKa = 11.84EE52 pKa = 3.78AHH54 pKa = 6.78KK55 pKa = 11.03DD56 pKa = 3.56GSLHH60 pKa = 6.61LHH62 pKa = 6.79ALLQTEE68 pKa = 4.27KK69 pKa = 10.2PVRR72 pKa = 11.84ISDD75 pKa = 3.96SRR77 pKa = 11.84FFDD80 pKa = 3.32INGFHH85 pKa = 7.46PNIQSAKK92 pKa = 8.27SVNRR96 pKa = 11.84VRR98 pKa = 11.84DD99 pKa = 3.85YY100 pKa = 11.14ILKK103 pKa = 10.0EE104 pKa = 3.56PLAVFEE110 pKa = 5.15RR111 pKa = 11.84GTFIPRR117 pKa = 11.84KK118 pKa = 9.9SPFLGKK124 pKa = 9.77SDD126 pKa = 4.69SEE128 pKa = 4.48VKK130 pKa = 9.84EE131 pKa = 4.11KK132 pKa = 10.86KK133 pKa = 9.48PSKK136 pKa = 10.42DD137 pKa = 2.99EE138 pKa = 3.71IMRR141 pKa = 11.84DD142 pKa = 3.68IISHH146 pKa = 5.57ATSKK150 pKa = 11.1EE151 pKa = 4.12EE152 pKa = 3.92YY153 pKa = 10.3LSMIQKK159 pKa = 8.82EE160 pKa = 4.59LPFDD164 pKa = 3.51WSTKK168 pKa = 8.61LQYY171 pKa = 10.45FEE173 pKa = 4.94YY174 pKa = 10.26SANKK178 pKa = 9.59LFPEE182 pKa = 4.01IQEE185 pKa = 4.39EE186 pKa = 4.56FTNPHH191 pKa = 6.82PPSSPDD197 pKa = 3.42LLCNEE202 pKa = 5.57SINDD206 pKa = 3.68WLQPNIFQVSPEE218 pKa = 4.27AYY220 pKa = 9.18MLLQPTCYY228 pKa = 9.44TLEE231 pKa = 4.25DD232 pKa = 5.23AISDD236 pKa = 4.27LQWMDD241 pKa = 3.46SVSSHH246 pKa = 5.65QMKK249 pKa = 10.2DD250 pKa = 3.22QEE252 pKa = 4.62SRR254 pKa = 11.84ASTSSAQQEE263 pKa = 4.43PEE265 pKa = 3.87NLLGPEE271 pKa = 3.97AA272 pKa = 4.79

Molecular weight: 31.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C649|MP_MSVN Movement protein OS=Maize streak virus genotype A (isolate Nigeria) OX=10823 GN=V2 PE=1 SV=1
MM1 pKa = 6.95STSKK5 pKa = 10.57RR6 pKa = 11.84KK7 pKa = 10.24RR8 pKa = 11.84GDD10 pKa = 3.09DD11 pKa = 3.93SNWSKK16 pKa = 11.06RR17 pKa = 11.84VTKK20 pKa = 10.25KK21 pKa = 10.55KK22 pKa = 10.09PSSAGLKK29 pKa = 9.76RR30 pKa = 11.84AGSKK34 pKa = 10.46ADD36 pKa = 3.86RR37 pKa = 11.84PSLQIQTLQHH47 pKa = 6.77AGTTMITVPSGGVCDD62 pKa = 6.17LINTYY67 pKa = 11.02ARR69 pKa = 11.84GSDD72 pKa = 3.04EE73 pKa = 4.39GNRR76 pKa = 11.84HH77 pKa = 4.54TSEE80 pKa = 3.76TLTYY84 pKa = 10.12KK85 pKa = 10.39IAIDD89 pKa = 3.49YY90 pKa = 10.75HH91 pKa = 6.41FVADD95 pKa = 4.59AAACRR100 pKa = 11.84YY101 pKa = 9.77SNTGTGVMWLVYY113 pKa = 9.18DD114 pKa = 4.16TTPGGQAPTPQTIFAYY130 pKa = 9.88PDD132 pKa = 3.36TLKK135 pKa = 10.77AWPATWKK142 pKa = 10.4VSRR145 pKa = 11.84EE146 pKa = 3.81LCHH149 pKa = 6.49RR150 pKa = 11.84FVVKK154 pKa = 10.43RR155 pKa = 11.84RR156 pKa = 11.84WLFNMEE162 pKa = 3.66TDD164 pKa = 3.45GRR166 pKa = 11.84IGSDD170 pKa = 3.03IPPSNASWKK179 pKa = 7.91PCKK182 pKa = 10.05RR183 pKa = 11.84NIYY186 pKa = 7.92FHH188 pKa = 7.28KK189 pKa = 9.03FTSGLGVRR197 pKa = 11.84TQWKK201 pKa = 8.76NVTDD205 pKa = 4.02GGVGAIQRR213 pKa = 11.84GALYY217 pKa = 9.56MVIAPGNGLTFTAHH231 pKa = 5.61GQTRR235 pKa = 11.84LYY237 pKa = 9.73FKK239 pKa = 10.92SVGNQQ244 pKa = 2.8

Molecular weight: 26.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

977

101

360

244.3

27.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.834 ± 0.79

1.842 ± 0.172

4.913 ± 0.276

5.937 ± 1.475

4.299 ± 0.323

5.118 ± 1.729

2.354 ± 0.344

6.141 ± 0.556

6.653 ± 0.909

7.165 ± 0.8

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.047 ± 0.206

4.811 ± 0.326

7.574 ± 0.966

4.504 ± 0.376

5.322 ± 0.527

9.314 ± 0.886

5.322 ± 1.459

4.811 ± 0.725

2.456 ± 0.241

3.582 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski