Torque teno mini virus 1 (isolate TLMV-CBD279)
Average proteome isoelectric point is 7.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9QU31|ORF2_TTVB1 Uncharacterized ORF2 protein OS=Torque teno mini virus 1 (isolate TLMV-CBD279) OX=766183 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.81 SSFLTPAVYY10 pKa = 10.46 SKK12 pKa = 11.37 NGLEE16 pKa = 4.1 NQWINTIWNTHH27 pKa = 6.1 DD28 pKa = 5.49 LMCGCNNAIKK38 pKa = 10.54 HH39 pKa = 5.99 LFDD42 pKa = 3.31 ILKK45 pKa = 10.55 KK46 pKa = 10.55 KK47 pKa = 10.77 GEE49 pKa = 4.07 QLCLPSTTEE58 pKa = 3.78 DD59 pKa = 4.06 AGTQTHH65 pKa = 6.31 GEE67 pKa = 4.12 EE68 pKa = 3.97 KK69 pKa = 10.5 DD70 pKa = 3.54 YY71 pKa = 11.44 DD72 pKa = 4.13 LEE74 pKa = 5.06 EE75 pKa = 5.85 GDD77 pKa = 5.55 LDD79 pKa = 4.7 ALFANDD85 pKa = 4.41 FEE87 pKa = 5.32 EE88 pKa = 6.12 DD89 pKa = 3.61 DD90 pKa = 4.0 GG91 pKa = 5.43
Molecular weight: 10.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.153
IPC2_protein 4.151
IPC_protein 4.075
Toseland 3.884
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.961
Rodwell 3.91
Grimsley 3.795
Solomon 4.037
Lehninger 3.999
Nozaki 4.164
DTASelect 4.355
Thurlkill 3.923
EMBOSS 3.973
Sillero 4.19
Patrickios 3.249
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.103
Protein with the highest isoelectric point:
>sp|Q9QU30|CAPSD_TTVB1 Capsid protein OS=Torque teno mini virus 1 (isolate TLMV-CBD279) OX=766183 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.34 TFYY4 pKa = 11.15 SSGEE8 pKa = 4.06 EE9 pKa = 4.25 AQHH12 pKa = 5.69 QWKK15 pKa = 9.2 QLQTQQNKK23 pKa = 9.37 KK24 pKa = 9.66 NSPRR28 pKa = 11.84 PVTSSRR34 pKa = 11.84 DD35 pKa = 3.38 FRR37 pKa = 11.84 SRR39 pKa = 11.84 VQGNQSNTIFTHH51 pKa = 5.09 LTKK54 pKa = 10.59 SAANSQCQLQKK65 pKa = 10.92 DD66 pKa = 4.11 LKK68 pKa = 10.2 KK69 pKa = 9.2 TLQLQHH75 pKa = 6.81 FLQTLEE81 pKa = 4.42 QKK83 pKa = 9.37 TSPSKK88 pKa = 10.62 HH89 pKa = 4.61 KK90 pKa = 10.47 RR91 pKa = 11.84 KK92 pKa = 7.63 RR93 pKa = 11.84 TKK95 pKa = 9.98 YY96 pKa = 10.17 RR97 pKa = 11.84 RR98 pKa = 11.84 TKK100 pKa = 10.13 KK101 pKa = 10.29 SKK103 pKa = 8.4 HH104 pKa = 5.41 HH105 pKa = 5.84 SRR107 pKa = 11.84 KK108 pKa = 8.51 KK109 pKa = 6.73 TTSSSSSSEE118 pKa = 3.76 RR119 pKa = 11.84 DD120 pKa = 3.09 STTGRR125 pKa = 11.84 EE126 pKa = 4.37 SEE128 pKa = 4.39 SSS130 pKa = 3.22
Molecular weight: 15.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.266
IPC2_protein 9.853
IPC_protein 10.365
Toseland 11.096
ProMoST 10.701
Dawson 11.14
Bjellqvist 10.789
Wikipedia 11.316
Rodwell 11.564
Grimsley 11.155
Solomon 11.272
Lehninger 11.242
Nozaki 11.067
DTASelect 10.789
Thurlkill 11.067
EMBOSS 11.491
Sillero 11.067
Patrickios 11.286
IPC_peptide 11.286
IPC2_peptide 9.443
IPC2.peptide.svr19 8.682
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
883
91
662
294.3
34.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.831 ± 0.554
1.359 ± 0.383
4.643 ± 1.232
6.002 ± 0.757
4.077 ± 0.276
4.304 ± 0.67
2.718 ± 0.685
5.21 ± 1.513
8.947 ± 1.066
9.173 ± 0.775
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.246 ± 0.184
4.757 ± 0.579
5.889 ± 1.786
6.002 ± 1.453
6.455 ± 1.274
7.361 ± 2.879
9.173 ± 0.605
2.605 ± 0.617
2.378 ± 0.53
4.87 ± 1.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here