Sphingobium sp. TKS

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126RQQ2|A0A126RQQ2_9SPHN Uncharacterized protein OS=Sphingobium sp. TKS OX=1315974 GN=K426_17410 PE=4 SV=1
MM1 pKa = 7.42SVTMSDD7 pKa = 3.17TALKK11 pKa = 10.48IGDD14 pKa = 3.86TSTVTVAFSEE24 pKa = 4.43AVTGFDD30 pKa = 3.36NSDD33 pKa = 3.2VTVEE37 pKa = 4.32HH38 pKa = 6.49GTLSTFTSLDD48 pKa = 3.43GKK50 pKa = 10.57VWTATFTPTANIEE63 pKa = 4.08DD64 pKa = 4.0SSNVVTVGSGYY75 pKa = 9.32TDD77 pKa = 3.56LAGNAGSGGTSANYY91 pKa = 10.57AIDD94 pKa = 3.66TKK96 pKa = 11.33APTLSITDD104 pKa = 3.83DD105 pKa = 3.37EE106 pKa = 4.66AAAVANNAGGAVVFTFTFSEE126 pKa = 4.4NVTGFTASDD135 pKa = 3.23ITVTGGTAGTFTAVNGHH152 pKa = 6.36SYY154 pKa = 9.33TLSVMPNADD163 pKa = 3.73SQSGTIAVSVADD175 pKa = 4.47GAATDD180 pKa = 3.93LAGNAVTGASASQNYY195 pKa = 7.68DD196 pKa = 3.19TLNPTVTVGFAGTALSDD213 pKa = 3.65GTASSQVNFVFSEE226 pKa = 4.51APVGFAQGDD235 pKa = 3.37ISASHH240 pKa = 6.18GAISNLVMDD249 pKa = 5.76DD250 pKa = 3.21ATHH253 pKa = 5.18YY254 pKa = 10.46HH255 pKa = 5.88ATFTADD261 pKa = 4.09DD262 pKa = 4.52GFSGTGSVTVDD273 pKa = 3.03AARR276 pKa = 11.84FTDD279 pKa = 4.48AALNDD284 pKa = 3.68NAAATPATIDD294 pKa = 3.64IDD296 pKa = 4.14TLNPTLVSVTMSDD309 pKa = 3.22TALKK313 pKa = 10.48IGDD316 pKa = 3.86TSTVTVAFSEE326 pKa = 4.43AVTGFDD332 pKa = 3.36NSDD335 pKa = 3.2VTVEE339 pKa = 4.32HH340 pKa = 6.49GTLSTFTSLDD350 pKa = 3.43GKK352 pKa = 10.57VWTATFTPTANIEE365 pKa = 4.08DD366 pKa = 4.0SSNVVTVGPNYY377 pKa = 10.15TDD379 pKa = 3.57LAGNAGSGGTSANYY393 pKa = 10.57AIDD396 pKa = 3.66TKK398 pKa = 11.33APTLSITDD406 pKa = 3.83DD407 pKa = 3.37EE408 pKa = 4.66AAAVANNAGGAVVFTFTFSEE428 pKa = 4.58DD429 pKa = 3.42VTGFTTDD436 pKa = 4.47DD437 pKa = 3.3ITVTGGTAGTFTAIDD452 pKa = 3.49GHH454 pKa = 6.82SYY456 pKa = 9.22TLSVTPDD463 pKa = 3.23ANNQAGTIAVSVADD477 pKa = 4.12GAAVDD482 pKa = 4.17GAGNPIIGSSASQNYY497 pKa = 7.68DD498 pKa = 3.17TLNPTLASVTMSDD511 pKa = 3.29SALKK515 pKa = 10.54IGDD518 pKa = 3.68TSIVTVTFSEE528 pKa = 4.5AVTGFDD534 pKa = 3.36NSDD537 pKa = 3.2VTVEE541 pKa = 4.32HH542 pKa = 6.49GTLSTFTSLDD552 pKa = 3.43GKK554 pKa = 10.57VWTATFTPTANIEE567 pKa = 4.11DD568 pKa = 4.34DD569 pKa = 4.37SNVVTVGSGYY579 pKa = 9.32TDD581 pKa = 3.57LAGNAGTGGTSANYY595 pKa = 10.19EE596 pKa = 4.22IDD598 pKa = 3.4TKK600 pKa = 11.42APTLSITDD608 pKa = 3.83DD609 pKa = 3.37EE610 pKa = 4.66AAAVANNAGGAVVFTFTFSEE630 pKa = 4.4NVTGFTASDD639 pKa = 3.23ITVTGGTAGTFTAVNGHH656 pKa = 6.41SYY658 pKa = 9.21TLSVTPDD665 pKa = 3.23ANNQAGTIGVSVANGAAMDD684 pKa = 4.17GAGNPIIGASASQNYY699 pKa = 7.48DD700 pKa = 3.1TKK702 pKa = 11.3NPTVAVNIIDD712 pKa = 4.62ASLDD716 pKa = 3.6NNDD719 pKa = 4.4PSSQVTFTFSEE730 pKa = 4.79TPVGFGSGDD739 pKa = 3.0ISAVGGTISNFQSTADD755 pKa = 3.82PLVYY759 pKa = 9.64TATYY763 pKa = 8.41TKK765 pKa = 9.89TAGFTGTGSVSVGSTLYY782 pKa = 10.46TDD784 pKa = 4.4AALNPGAPGSDD795 pKa = 2.99TVAVSSGDD803 pKa = 3.47TTSPGITSITGLQGNNNQNSKK824 pKa = 11.12LIITFNEE831 pKa = 3.66AMNTSFFDD839 pKa = 3.17ASKK842 pKa = 10.31IHH844 pKa = 6.28LAAATGTASIVTGTGAWSSNNTVFTIEE871 pKa = 4.01VAKK874 pKa = 10.47TNGANNGTSYY884 pKa = 9.77TAAIDD889 pKa = 3.35AGAFKK894 pKa = 10.69DD895 pKa = 3.9VAGNLSNAAQASGLKK910 pKa = 9.26PAGVAGAPINLGLSSPADD928 pKa = 3.31QAGDD932 pKa = 3.43VLVTVTHH939 pKa = 6.37MPDD942 pKa = 2.8DD943 pKa = 3.53WALNGGTRR951 pKa = 11.84NADD954 pKa = 3.45GSWTVLTADD963 pKa = 3.82PRR965 pKa = 11.84ALSVTTPSTFAGAMVLDD982 pKa = 4.56VAMSWTQADD991 pKa = 3.56GAMATMHH998 pKa = 6.68IADD1001 pKa = 4.21NVEE1004 pKa = 4.57AYY1006 pKa = 10.52APGNPIFAWSGDD1018 pKa = 3.64DD1019 pKa = 3.44TLTGSAQADD1028 pKa = 3.23IFVFAQPIGADD1039 pKa = 3.33QVHH1042 pKa = 6.98DD1043 pKa = 4.0FDD1045 pKa = 5.45IGADD1049 pKa = 3.81RR1050 pKa = 11.84IDD1052 pKa = 4.58LIGYY1056 pKa = 9.63AGIGDD1061 pKa = 4.18FSGVLAHH1068 pKa = 6.97LAEE1071 pKa = 4.89DD1072 pKa = 3.69ASGNALLTLAEE1083 pKa = 4.54GQTITLQGVTAAALTTANFLFDD1105 pKa = 3.71EE1106 pKa = 4.85TPVVSNSGVMTIGNGAMLPLSGTIVNSGVISLEE1139 pKa = 4.11STGDD1143 pKa = 3.35TTLLQLIQYY1152 pKa = 7.63GTTLQGGGHH1161 pKa = 5.59VVLSDD1166 pKa = 3.61DD1167 pKa = 4.1GGNVISGTLPSVTLNNVDD1185 pKa = 4.44NIISGAGEE1193 pKa = 3.88IGAGTLTLINGGSIIADD1210 pKa = 3.63GTHH1213 pKa = 6.48GLVLDD1218 pKa = 4.47TGTNMISNSGIIAATGSGGLTVVSAIDD1245 pKa = 3.66NEE1247 pKa = 4.4GLIQLNSSHH1256 pKa = 6.5AVLVGDD1262 pKa = 3.82VSGSGDD1268 pKa = 3.51VVLSGTATVEE1278 pKa = 4.17FGGHH1282 pKa = 4.05VTSDD1286 pKa = 2.86IVVDD1290 pKa = 4.59AGATGLILFDD1300 pKa = 5.1DD1301 pKa = 5.11SISFSGTIAGLDD1313 pKa = 3.22SDD1315 pKa = 5.3DD1316 pKa = 5.52RR1317 pKa = 11.84VDD1319 pKa = 5.22LSDD1322 pKa = 4.44LAFDD1326 pKa = 4.16AATMLSYY1333 pKa = 10.8AANDD1337 pKa = 3.59TGTGGVLTVTSGADD1351 pKa = 3.43VAHH1354 pKa = 6.48LTLIGSYY1361 pKa = 9.76HH1362 pKa = 7.39AEE1364 pKa = 4.51DD1365 pKa = 3.76FGLWSDD1371 pKa = 3.79GHH1373 pKa = 6.99SGTLISFGG1381 pKa = 3.65

Molecular weight: 137.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126RQT2|A0A126RQT2_9SPHN HTH_17 domain-containing protein OS=Sphingobium sp. TKS OX=1315974 GN=K426_18505 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5795

0

5795

1774848

23

7080

306.3

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.908 ± 0.046

0.848 ± 0.011

5.941 ± 0.026

5.466 ± 0.035

3.494 ± 0.02

8.794 ± 0.103

2.138 ± 0.021

5.182 ± 0.021

3.053 ± 0.025

10.024 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.52 ± 0.018

2.539 ± 0.022

5.189 ± 0.032

3.263 ± 0.019

7.511 ± 0.047

5.491 ± 0.032

5.095 ± 0.031

6.919 ± 0.03

1.415 ± 0.015

2.21 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski