Bornean orang-utan polyomavirus
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C9X3W4|C9X3W4_9POLY Minor capsid protein VP2 OS=Bornean orang-utan polyomavirus OX=1604874 GN=VP3 PE=3 SV=1
MM1 pKa = 7.47 GGVISLFFDD10 pKa = 3.03 IVEE13 pKa = 4.39 ISAEE17 pKa = 4.01 LSTATGFAVDD27 pKa = 3.86 AVLTGEE33 pKa = 3.94 AAAAVEE39 pKa = 4.36 TEE41 pKa = 4.29 VMGLMTIEE49 pKa = 4.43 GLSASEE55 pKa = 4.18 ALGAMGLTMEE65 pKa = 5.37 DD66 pKa = 3.84 FSLMHH71 pKa = 7.26 ALPGMLSEE79 pKa = 4.54 AVGIGTLFQAISGASGLVAAGIRR102 pKa = 11.84 YY103 pKa = 9.05 GYY105 pKa = 10.05 ARR107 pKa = 11.84 EE108 pKa = 3.77 VSIVNRR114 pKa = 11.84 NKK116 pKa = 10.49 RR117 pKa = 11.84 NPSEE121 pKa = 3.93 MALQVWRR128 pKa = 11.84 PWDD131 pKa = 3.54 YY132 pKa = 11.83 YY133 pKa = 11.28 DD134 pKa = 4.6 ILFPGVNTFAHH145 pKa = 6.17 YY146 pKa = 11.16 LNILDD151 pKa = 4.79 DD152 pKa = 3.9 WASSLIHH159 pKa = 5.36 TVSRR163 pKa = 11.84 YY164 pKa = 7.19 VWDD167 pKa = 5.59 AILHH171 pKa = 5.7 EE172 pKa = 4.69 SRR174 pKa = 11.84 HH175 pKa = 5.42 QIGHH179 pKa = 7.09 ASRR182 pKa = 11.84 EE183 pKa = 4.1 LVARR187 pKa = 11.84 GSGHH191 pKa = 6.03 FQDD194 pKa = 5.13 LMARR198 pKa = 11.84 LIEE201 pKa = 3.94 NSRR204 pKa = 11.84 WVLTTGPSNVYY215 pKa = 10.12 SHH217 pKa = 7.1 LEE219 pKa = 3.67 SYY221 pKa = 10.31 YY222 pKa = 10.22 RR223 pKa = 11.84 QLPGISPPQARR234 pKa = 11.84 DD235 pKa = 3.15 LYY237 pKa = 11.02 RR238 pKa = 11.84 RR239 pKa = 11.84 LQEE242 pKa = 4.67 KK243 pKa = 10.35 IPDD246 pKa = 3.54 RR247 pKa = 11.84 HH248 pKa = 5.44 QLAVATDD255 pKa = 3.81 EE256 pKa = 4.14 SAEE259 pKa = 4.18 VIEE262 pKa = 5.07 TYY264 pKa = 9.25 PAPGGAHH271 pKa = 5.75 QRR273 pKa = 11.84 VCPDD277 pKa = 2.28 WMLPLVLGLYY287 pKa = 10.89 GDD289 pKa = 3.78 ITPTFGEE296 pKa = 4.38 YY297 pKa = 9.83 LRR299 pKa = 11.84 KK300 pKa = 9.72 EE301 pKa = 4.37 DD302 pKa = 3.8 GPQKK306 pKa = 10.2 KK307 pKa = 9.18 RR308 pKa = 11.84 RR309 pKa = 11.84 RR310 pKa = 11.84 LL311 pKa = 3.33
Molecular weight: 34.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.306
IPC2_protein 5.296
IPC_protein 5.283
Toseland 5.512
ProMoST 5.525
Dawson 5.397
Bjellqvist 5.474
Wikipedia 5.334
Rodwell 5.359
Grimsley 5.588
Solomon 5.397
Lehninger 5.372
Nozaki 5.588
DTASelect 5.753
Thurlkill 5.614
EMBOSS 5.563
Sillero 5.677
Patrickios 3.935
IPC_peptide 5.41
IPC2_peptide 5.69
IPC2.peptide.svr19 5.623
Protein with the highest isoelectric point:
>tr|C9X3W7|C9X3W7_9POLY Small T antigen OS=Bornean orang-utan polyomavirus OX=1604874 GN=small T PE=4 SV=1
MM1 pKa = 7.9 DD2 pKa = 5.14 RR3 pKa = 11.84 FLSRR7 pKa = 11.84 EE8 pKa = 3.77 EE9 pKa = 4.32 SNEE12 pKa = 3.71 LMEE15 pKa = 5.31 LLNIPKK21 pKa = 9.59 HH22 pKa = 6.19 CYY24 pKa = 10.64 GNFALMKK31 pKa = 9.1 INHH34 pKa = 6.7 RR35 pKa = 11.84 KK36 pKa = 8.83 MSLKK40 pKa = 9.85 YY41 pKa = 10.36 HH42 pKa = 6.84 PDD44 pKa = 3.2 KK45 pKa = 11.6 GGDD48 pKa = 3.64 PEE50 pKa = 5.33 KK51 pKa = 10.19 MARR54 pKa = 11.84 LNALWQKK61 pKa = 10.07 LQEE64 pKa = 4.64 GIYY67 pKa = 9.98 NARR70 pKa = 11.84 QEE72 pKa = 4.4 YY73 pKa = 6.51 PTSFGSKK80 pKa = 7.83 VGSWFWEE87 pKa = 4.03 ANLITLKK94 pKa = 10.47 EE95 pKa = 4.17 YY96 pKa = 9.7 YY97 pKa = 10.08 GKK99 pKa = 10.64 RR100 pKa = 11.84 KK101 pKa = 9.54 FDD103 pKa = 3.69 EE104 pKa = 4.2 NLIKK108 pKa = 10.33 HH109 pKa = 6.16 WPQCAEE115 pKa = 4.47 KK116 pKa = 10.54 ALQEE120 pKa = 4.59 CKK122 pKa = 10.67 CLTCKK127 pKa = 10.55 LGLQHH132 pKa = 6.62 QVYY135 pKa = 9.86 KK136 pKa = 10.87 QMHH139 pKa = 4.88 QKK141 pKa = 10.21 KK142 pKa = 9.29 CIVWGEE148 pKa = 4.14 CFCFKK153 pKa = 10.49 CYY155 pKa = 10.45 CIWFGRR161 pKa = 11.84 GPLLSGFLVGMEE173 pKa = 4.51 CNCGRR178 pKa = 11.84 SGLSSGEE185 pKa = 4.23 HH186 pKa = 5.44 ISEE189 pKa = 4.05 NKK191 pKa = 9.16 PRR193 pKa = 11.84 PKK195 pKa = 10.49 LGG197 pKa = 3.3
Molecular weight: 22.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.053
IPC2_protein 8.112
IPC_protein 7.936
Toseland 7.614
ProMoST 8.521
Dawson 8.712
Bjellqvist 9.033
Wikipedia 8.639
Rodwell 8.756
Grimsley 7.556
Solomon 8.799
Lehninger 8.799
Nozaki 9.311
DTASelect 8.741
Thurlkill 8.829
EMBOSS 8.902
Sillero 9.151
Patrickios 4.24
IPC_peptide 8.785
IPC2_peptide 8.009
IPC2.peptide.svr19 8.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1757
190
693
351.4
39.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.464 ± 1.141
2.447 ± 0.692
5.236 ± 0.543
7.057 ± 0.706
4.269 ± 0.713
6.659 ± 1.195
2.675 ± 0.437
4.326 ± 0.412
6.261 ± 1.375
10.074 ± 0.764
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.903 ± 0.468
4.496 ± 0.74
6.033 ± 0.686
3.813 ± 0.226
5.236 ± 0.652
6.261 ± 0.353
5.065 ± 0.697
5.976 ± 0.741
1.651 ± 0.362
4.098 ± 0.436
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here