Dragonfly larvae associated circular virus-5
Average proteome isoelectric point is 7.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5U1V9|W5U1V9_9VIRU Coat protein OS=Dragonfly larvae associated circular virus-5 OX=1454026 PE=4 SV=1
MM1 pKa = 7.89 ADD3 pKa = 4.95 LLPAQQKK10 pKa = 10.34 KK11 pKa = 9.45 IAPTQKK17 pKa = 9.73 WKK19 pKa = 10.38 DD20 pKa = 3.29 QRR22 pKa = 11.84 GRR24 pKa = 11.84 CWCFTAFVDD33 pKa = 5.97 RR34 pKa = 11.84 PWKK37 pKa = 10.62 DD38 pKa = 3.06 IACKK42 pKa = 10.27 YY43 pKa = 9.7 IVVGHH48 pKa = 6.61 EE49 pKa = 3.83 ICPKK53 pKa = 8.81 TGKK56 pKa = 8.33 EE57 pKa = 3.43 HH58 pKa = 5.07 WQGYY62 pKa = 7.35 VEE64 pKa = 5.64 FGTQRR69 pKa = 11.84 RR70 pKa = 11.84 PTGVQKK76 pKa = 9.64 TLGIPDD82 pKa = 3.43 LHH84 pKa = 7.57 VEE86 pKa = 4.06 KK87 pKa = 10.65 RR88 pKa = 11.84 LDD90 pKa = 3.44 TSTPDD95 pKa = 3.38 HH96 pKa = 6.67 AANYY100 pKa = 7.99 CKK102 pKa = 10.32 KK103 pKa = 10.52 DD104 pKa = 3.5 MKK106 pKa = 10.45 FVEE109 pKa = 4.2 YY110 pKa = 10.78 GKK112 pKa = 10.11 ISKK115 pKa = 10.06 QGEE118 pKa = 4.28 RR119 pKa = 11.84 MDD121 pKa = 5.5 LDD123 pKa = 5.23 LICHH127 pKa = 6.93 DD128 pKa = 4.3 LQTGKK133 pKa = 8.87 RR134 pKa = 11.84 TWRR137 pKa = 11.84 EE138 pKa = 3.26 IMGSDD143 pKa = 4.92 DD144 pKa = 3.43 VQTVCKK150 pKa = 10.46 YY151 pKa = 10.99 RR152 pKa = 11.84 MGLQMVAAEE161 pKa = 4.37 SAAEE165 pKa = 3.93 RR166 pKa = 11.84 AKK168 pKa = 10.65 KK169 pKa = 9.35 PRR171 pKa = 11.84 EE172 pKa = 4.07 VTWEE176 pKa = 4.19 RR177 pKa = 11.84 KK178 pKa = 9.42 PSMAAAWLDD187 pKa = 3.7 YY188 pKa = 10.82 PDD190 pKa = 4.77 ALMVNLNNGACWEE203 pKa = 4.25 AYY205 pKa = 10.03 GGEE208 pKa = 4.0 EE209 pKa = 4.34 EE210 pKa = 5.38 IIVRR214 pKa = 11.84 HH215 pKa = 5.43 AKK217 pKa = 9.86 SYY219 pKa = 10.48 HH220 pKa = 6.47 RR221 pKa = 11.84 EE222 pKa = 3.85 LLLDD226 pKa = 3.78 GWITPLNVKK235 pKa = 8.74 CTTKK239 pKa = 10.1 YY240 pKa = 10.48 AAWTKK245 pKa = 8.69 VICVEE250 pKa = 4.27 CDD252 pKa = 3.14 PEE254 pKa = 4.21 FFPPVEE260 pKa = 4.14 PVSEE264 pKa = 4.53 VVSEE268 pKa = 4.22 VPPPLGNTIPGVEE281 pKa = 4.3 VPAVKK286 pKa = 10.56 DD287 pKa = 3.77 EE288 pKa = 4.25 EE289 pKa = 5.13 ASALPP294 pKa = 4.0
Molecular weight: 33.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.981
IPC2_protein 6.046
IPC_protein 6.097
Toseland 6.249
ProMoST 6.504
Dawson 6.415
Bjellqvist 6.415
Wikipedia 6.427
Rodwell 6.415
Grimsley 6.326
Solomon 6.427
Lehninger 6.415
Nozaki 6.693
DTASelect 6.839
Thurlkill 6.868
EMBOSS 6.839
Sillero 6.81
Patrickios 4.177
IPC_peptide 6.44
IPC2_peptide 6.664
IPC2.peptide.svr19 6.664
Protein with the highest isoelectric point:
>tr|W5U1V9|W5U1V9_9VIRU Coat protein OS=Dragonfly larvae associated circular virus-5 OX=1454026 PE=4 SV=1
MM1 pKa = 6.89 VFRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 VSRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 ASRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 VRR19 pKa = 11.84 RR20 pKa = 11.84 TSRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 PRR27 pKa = 11.84 ARR29 pKa = 11.84 RR30 pKa = 11.84 GRR32 pKa = 11.84 RR33 pKa = 11.84 NGGNSIMSSSIRR45 pKa = 11.84 MPHH48 pKa = 6.7 KK49 pKa = 9.42 YY50 pKa = 10.35 SEE52 pKa = 4.26 IFGLTFTGGSGVAGIYY68 pKa = 9.75 QFYY71 pKa = 10.73 LNYY74 pKa = 10.15 LEE76 pKa = 4.96 HH77 pKa = 7.11 PGYY80 pKa = 9.3 TGAGHH85 pKa = 6.04 QPMGFDD91 pKa = 4.06 QISALFNRR99 pKa = 11.84 YY100 pKa = 8.15 RR101 pKa = 11.84 VIGMSYY107 pKa = 10.38 HH108 pKa = 6.49 IALTNISGVYY118 pKa = 7.26 VAEE121 pKa = 3.9 YY122 pKa = 10.27 AVYY125 pKa = 10.0 YY126 pKa = 9.17 RR127 pKa = 11.84 PNMTVTNDD135 pKa = 2.9 FTAIMEE141 pKa = 4.47 GTGVLAKK148 pKa = 10.07 GHH150 pKa = 6.63 LGITSSGSDD159 pKa = 2.98 TRR161 pKa = 11.84 THH163 pKa = 6.59 RR164 pKa = 11.84 GYY166 pKa = 11.35 VSVARR171 pKa = 11.84 ARR173 pKa = 11.84 GVPRR177 pKa = 11.84 RR178 pKa = 11.84 NVMTEE183 pKa = 3.24 SDD185 pKa = 3.82 YY186 pKa = 11.38 QAILGVAPNFLPSFNIAVVNQDD208 pKa = 2.71 ASASVTLRR216 pKa = 11.84 VRR218 pKa = 11.84 VDD220 pKa = 3.12 LKK222 pKa = 11.1 FYY224 pKa = 9.06 TIWSDD229 pKa = 3.36 RR230 pKa = 11.84 KK231 pKa = 10.96 VMGTSS236 pKa = 2.98
Molecular weight: 26.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.176
IPC2_protein 10.365
IPC_protein 11.418
Toseland 11.403
ProMoST 11.857
Dawson 11.433
Bjellqvist 11.403
Wikipedia 11.886
Rodwell 11.14
Grimsley 11.491
Solomon 11.886
Lehninger 11.784
Nozaki 11.403
DTASelect 11.403
Thurlkill 11.403
EMBOSS 11.886
Sillero 11.418
Patrickios 10.804
IPC_peptide 11.886
IPC2_peptide 10.862
IPC2.peptide.svr19 8.979
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
530
236
294
265.0
29.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.736 ± 0.351
2.075 ± 1.367
4.717 ± 1.153
5.472 ± 2.208
3.019 ± 0.802
7.736 ± 1.324
2.453 ± 0.059
4.906 ± 0.397
5.472 ± 2.487
5.472 ± 0.534
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.208 ± 0.399
3.208 ± 0.957
5.472 ± 1.371
3.019 ± 0.593
9.057 ± 3.245
5.849 ± 2.287
6.226 ± 0.364
8.491 ± 0.269
2.075 ± 1.088
4.34 ± 1.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here