Rhizoctonia solani dsRNA virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus; unclassified Alphapartitivirus

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143BRM9|A0A143BRM9_9VIRU Coat protein OS=Rhizoctonia solani dsRNA virus 3 OX=1825688 PE=4 SV=1
MM1 pKa = 7.81FYY3 pKa = 8.23TTISTIKK10 pKa = 10.51VFLKK14 pKa = 10.71NLLFSDD20 pKa = 3.25PHH22 pKa = 7.74KK23 pKa = 10.56FVNNFQFIGYY33 pKa = 9.11ASDD36 pKa = 4.38RR37 pKa = 11.84IRR39 pKa = 11.84VSIPYY44 pKa = 9.31RR45 pKa = 11.84DD46 pKa = 3.27EE47 pKa = 4.07FQYY50 pKa = 11.08EE51 pKa = 4.34RR52 pKa = 11.84YY53 pKa = 9.44QRR55 pKa = 11.84TVRR58 pKa = 11.84HH59 pKa = 5.55ALRR62 pKa = 11.84RR63 pKa = 11.84NLIGYY68 pKa = 8.77DD69 pKa = 2.96AEE71 pKa = 4.39YY72 pKa = 10.06IIKK75 pKa = 9.88EE76 pKa = 3.92FHH78 pKa = 6.91HH79 pKa = 6.65PVANLDD85 pKa = 3.73FMVDD89 pKa = 3.45ALRR92 pKa = 11.84KK93 pKa = 9.86GDD95 pKa = 4.42LPDD98 pKa = 3.53HH99 pKa = 6.66VIPKK103 pKa = 9.82DD104 pKa = 3.45EE105 pKa = 4.91HH106 pKa = 6.25YY107 pKa = 11.44SKK109 pKa = 11.38AFAQAAEE116 pKa = 4.41MFRR119 pKa = 11.84PPQLVRR125 pKa = 11.84PVHH128 pKa = 5.75FADD131 pKa = 3.7LRR133 pKa = 11.84MYY135 pKa = 9.65KK136 pKa = 9.8WNWHH140 pKa = 6.25PNVEE144 pKa = 4.19EE145 pKa = 4.41PFYY148 pKa = 11.41SDD150 pKa = 3.76ADD152 pKa = 4.45LIRR155 pKa = 11.84AVSMAAEE162 pKa = 4.65AGLLPDD168 pKa = 4.08ARR170 pKa = 11.84MSFGNLRR177 pKa = 11.84NVVFIKK183 pKa = 10.72ARR185 pKa = 11.84LFLHH189 pKa = 5.67QIKK192 pKa = 10.19RR193 pKa = 11.84KK194 pKa = 9.95QITNPATLWPMMKK207 pKa = 9.27IHH209 pKa = 6.67VKK211 pKa = 9.79PALTKK216 pKa = 9.81VDD218 pKa = 3.63EE219 pKa = 4.5TKK221 pKa = 10.66VRR223 pKa = 11.84IIYY226 pKa = 9.35GVSKK230 pKa = 9.92LHH232 pKa = 6.64VMAQAMFLWPLFNYY246 pKa = 9.38YY247 pKa = 9.84INSDD251 pKa = 4.49DD252 pKa = 6.37DD253 pKa = 3.99PLLWGFEE260 pKa = 4.31TILGGMQKK268 pKa = 9.88LHH270 pKa = 7.13NIMSIPRR277 pKa = 11.84LYY279 pKa = 10.17FQTFVTVDD287 pKa = 2.64WSGFDD292 pKa = 3.49LRR294 pKa = 11.84SVFSLQRR301 pKa = 11.84EE302 pKa = 4.36VFDD305 pKa = 3.01VWRR308 pKa = 11.84TYY310 pKa = 11.16FDD312 pKa = 4.11FNNGYY317 pKa = 9.67IPTKK321 pKa = 10.36FYY323 pKa = 9.14RR324 pKa = 11.84TSVADD329 pKa = 3.97PDD331 pKa = 3.83HH332 pKa = 7.41LEE334 pKa = 5.5ALWEE338 pKa = 4.14WQRR341 pKa = 11.84EE342 pKa = 4.43ACFKK346 pKa = 10.54MPFVMPDD353 pKa = 2.46RR354 pKa = 11.84TMYY357 pKa = 10.72NRR359 pKa = 11.84LFRR362 pKa = 11.84CIPSGLFSTQFLDD375 pKa = 3.51SHH377 pKa = 6.93VNLVMILTILDD388 pKa = 3.56AMHH391 pKa = 6.96FDD393 pKa = 3.03ISKK396 pKa = 10.2IKK398 pKa = 10.3IYY400 pKa = 10.73VQGDD404 pKa = 3.46DD405 pKa = 4.77SIVMLIFHH413 pKa = 7.06IPADD417 pKa = 3.41QHH419 pKa = 7.02IKK421 pKa = 10.34FKK423 pKa = 11.31SDD425 pKa = 3.36FEE427 pKa = 4.47VLAKK431 pKa = 10.76YY432 pKa = 10.69YY433 pKa = 9.45FDD435 pKa = 3.64HH436 pKa = 6.55VARR439 pKa = 11.84PEE441 pKa = 3.97KK442 pKa = 9.78TDD444 pKa = 3.36VYY446 pKa = 9.02EE447 pKa = 4.34TPQGVEE453 pKa = 3.51VLGYY457 pKa = 10.28RR458 pKa = 11.84NYY460 pKa = 10.93NGYY463 pKa = 8.78PEE465 pKa = 4.74RR466 pKa = 11.84DD467 pKa = 3.11WRR469 pKa = 11.84KK470 pKa = 9.62LLAQLLHH477 pKa = 6.34PRR479 pKa = 11.84GALSLEE485 pKa = 4.03TLAARR490 pKa = 11.84CCGIAYY496 pKa = 10.26ASMYY500 pKa = 10.46RR501 pKa = 11.84NPEE504 pKa = 4.06VINVCKK510 pKa = 10.51DD511 pKa = 2.59IYY513 pKa = 11.35NYY515 pKa = 9.24LTTKK519 pKa = 10.44RR520 pKa = 11.84NVVPGEE526 pKa = 3.78LRR528 pKa = 11.84AQRR531 pKa = 11.84DD532 pKa = 3.42IILFGEE538 pKa = 4.58HH539 pKa = 6.49EE540 pKa = 4.38FSIPTDD546 pKa = 3.42HH547 pKa = 7.1FPEE550 pKa = 5.88RR551 pKa = 11.84DD552 pKa = 3.13EE553 pKa = 4.26VTRR556 pKa = 11.84HH557 pKa = 4.99LRR559 pKa = 11.84IPYY562 pKa = 9.45VRR564 pKa = 11.84TDD566 pKa = 3.04SDD568 pKa = 4.24KK569 pKa = 11.34NDD571 pKa = 3.49YY572 pKa = 9.13WPSGHH577 pKa = 6.64FLSLYY582 pKa = 10.48

Molecular weight: 68.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143BRM9|A0A143BRM9_9VIRU Coat protein OS=Rhizoctonia solani dsRNA virus 3 OX=1825688 PE=4 SV=1
MM1 pKa = 7.89SDD3 pKa = 3.57PAIKK7 pKa = 10.61SIDD10 pKa = 3.52DD11 pKa = 3.29VMSYY15 pKa = 8.75VRR17 pKa = 11.84KK18 pKa = 9.71QFEE21 pKa = 4.3EE22 pKa = 3.95MRR24 pKa = 11.84SFQQAATPPVPEE36 pKa = 4.39KK37 pKa = 11.01KK38 pKa = 9.58EE39 pKa = 3.81ARR41 pKa = 11.84DD42 pKa = 3.41ALGQRR47 pKa = 11.84DD48 pKa = 4.11VVAQAQKK55 pKa = 9.97ATTASASKK63 pKa = 10.54PPGPSSAPDD72 pKa = 3.26VPVPFVHH79 pKa = 7.07TSDD82 pKa = 3.68NVTNSLRR89 pKa = 11.84LALGLNFRR97 pKa = 11.84ATPVPQFGSNWYY109 pKa = 9.3LPSYY113 pKa = 9.95FLSHH117 pKa = 7.03QILDD121 pKa = 3.35ILNRR125 pKa = 11.84KK126 pKa = 7.98VFSVRR131 pKa = 11.84KK132 pKa = 9.01FYY134 pKa = 10.52EE135 pKa = 3.88ASEE138 pKa = 4.08FLDD141 pKa = 3.67PLLVHH146 pKa = 6.58VYY148 pKa = 10.01IQILEE153 pKa = 4.55IIHH156 pKa = 5.96TLRR159 pKa = 11.84AQQQGSTISTEE170 pKa = 4.04NEE172 pKa = 3.8LFLSWFDD179 pKa = 3.94NNFPSDD185 pKa = 3.87TLPVPGTHH193 pKa = 5.26VTLIANLAASSPAIGSYY210 pKa = 11.21SNIFPRR216 pKa = 11.84LPDD219 pKa = 3.9DD220 pKa = 4.75SRR222 pKa = 11.84ATTNTNGVPTTNAATGTMHH241 pKa = 6.92QLTGRR246 pKa = 11.84LVPIPGILDD255 pKa = 3.59VYY257 pKa = 9.52RR258 pKa = 11.84TRR260 pKa = 11.84LVLAINQINAQSPSTMTVGNFNLAHH285 pKa = 5.46RR286 pKa = 11.84TVLNLQCLTGAEE298 pKa = 4.09NSFTGPYY305 pKa = 8.51YY306 pKa = 9.62QAPVAAGLPATVAYY320 pKa = 9.59FSSPGVITAPWMSATAASSFMNNSLYY346 pKa = 10.1AQAMFGEE353 pKa = 4.98PSTANRR359 pKa = 11.84SEE361 pKa = 5.7DD362 pKa = 3.6FLSWQSFCGLDD373 pKa = 5.24GDD375 pKa = 4.63TQWFTEE381 pKa = 4.47TARR384 pKa = 11.84LMTVYY389 pKa = 10.51CKK391 pKa = 10.04FVKK394 pKa = 10.73GSAPLSAIPFSGHH407 pKa = 5.08MANHH411 pKa = 5.45VTWSPSSAPYY421 pKa = 10.5GPATHH426 pKa = 7.73RR427 pKa = 11.84YY428 pKa = 8.51RR429 pKa = 11.84EE430 pKa = 4.36RR431 pKa = 11.84ISMAGNGSVQPRR443 pKa = 11.84YY444 pKa = 9.3PRR446 pKa = 11.84SRR448 pKa = 11.84HH449 pKa = 4.66CC450 pKa = 4.62

Molecular weight: 49.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1032

450

582

516.0

58.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.752 ± 1.427

0.872 ± 0.011

5.62 ± 1.17

3.973 ± 0.547

6.202 ± 0.692

4.36 ± 0.618

3.295 ± 0.54

5.523 ± 0.826

3.779 ± 0.988

8.43 ± 0.414

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.907 ± 0.294

4.651 ± 0.433

6.783 ± 0.914

3.779 ± 0.563

6.202 ± 0.692

7.074 ± 1.999

5.911 ± 1.326

6.686 ± 0.153

1.647 ± 0.199

4.554 ± 0.775
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski