Microbacterium phage Leaf

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rogerhendrixvirus; unclassified Rogerhendrixvirus

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9JDG2|A0A6B9JDG2_9CAUD Uncharacterized protein OS=Microbacterium phage Leaf OX=2686087 GN=75 PE=4 SV=1
MM1 pKa = 8.03PINDD5 pKa = 4.11ALLNAGATAMGTAAAYY21 pKa = 10.26ASIHH25 pKa = 5.04TAQPNAAGNNEE36 pKa = 3.98STAARR41 pKa = 11.84QLIDD45 pKa = 3.33WSTASAGDD53 pKa = 3.82LALDD57 pKa = 3.99APLAFTGGAAGGPATHH73 pKa = 7.06LGLWSAPTAGTFYY86 pKa = 10.85GYY88 pKa = 10.76FPLTGDD94 pKa = 3.45QAFNAAGEE102 pKa = 4.34FTVTALALTGSATGAA117 pKa = 3.43

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9JAS0|A0A6B9JAS0_9CAUD Tape measure protein OS=Microbacterium phage Leaf OX=2686087 GN=76 PE=4 SV=1
MM1 pKa = 7.8GIFGINWSRR10 pKa = 11.84QIRR13 pKa = 11.84RR14 pKa = 11.84ATTKK18 pKa = 10.05AVKK21 pKa = 10.38GGTRR25 pKa = 11.84WASKK29 pKa = 9.93PYY31 pKa = 10.24RR32 pKa = 11.84EE33 pKa = 4.18QKK35 pKa = 10.47RR36 pKa = 11.84NNKK39 pKa = 8.79AAARR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 6.76TWRR49 pKa = 11.84ARR51 pKa = 3.27

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

156

0

156

30325

40

1332

194.4

21.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.417 ± 0.339

0.805 ± 0.092

6.315 ± 0.232

7.73 ± 0.384

2.961 ± 0.133

8.185 ± 0.302

1.926 ± 0.126

4.452 ± 0.181

3.631 ± 0.207

7.832 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.109

2.988 ± 0.149

5.56 ± 0.246

3.766 ± 0.133

6.76 ± 0.286

5.454 ± 0.231

6.473 ± 0.271

7.268 ± 0.205

2.325 ± 0.137

2.542 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski