Amino acid dipepetide frequency for Lactobacillus phage PL-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.174AlaAla: 8.174 ± 2.304
0.458AlaCys: 0.458 ± 0.176
7.104AlaAsp: 7.104 ± 1.032
4.889AlaGlu: 4.889 ± 0.515
3.437AlaPhe: 3.437 ± 0.509
5.653AlaGly: 5.653 ± 0.855
1.222AlaHis: 1.222 ± 0.355
4.583AlaIle: 4.583 ± 0.719
7.104AlaLys: 7.104 ± 1.062
6.799AlaLeu: 6.799 ± 0.723
3.208AlaMet: 3.208 ± 0.635
5.194AlaAsn: 5.194 ± 0.65
1.833AlaPro: 1.833 ± 0.317
3.437AlaGln: 3.437 ± 0.509
2.674AlaArg: 2.674 ± 0.343
5.5AlaSer: 5.5 ± 1.025
4.965AlaThr: 4.965 ± 0.546
5.729AlaVal: 5.729 ± 0.726
0.687AlaTrp: 0.687 ± 0.319
3.972AlaTyr: 3.972 ± 0.61
0.0AlaXaa: 0.0 ± 0.0
Cys
0.382CysAla: 0.382 ± 0.162
0.076CysCys: 0.076 ± 0.077
0.229CysAsp: 0.229 ± 0.133
0.229CysGlu: 0.229 ± 0.139
0.153CysPhe: 0.153 ± 0.092
0.764CysGly: 0.764 ± 0.255
0.153CysHis: 0.153 ± 0.123
0.153CysIle: 0.153 ± 0.153
0.229CysLys: 0.229 ± 0.126
0.306CysLeu: 0.306 ± 0.181
0.076CysMet: 0.076 ± 0.073
0.0CysAsn: 0.0 ± 0.0
0.076CysPro: 0.076 ± 0.077
0.229CysGln: 0.229 ± 0.132
0.382CysArg: 0.382 ± 0.184
0.229CysSer: 0.229 ± 0.138
0.229CysThr: 0.229 ± 0.127
0.382CysVal: 0.382 ± 0.18
0.153CysTrp: 0.153 ± 0.094
0.153CysTyr: 0.153 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
5.5AspAla: 5.5 ± 0.609
0.153AspCys: 0.153 ± 0.112
5.424AspAsp: 5.424 ± 0.815
3.896AspGlu: 3.896 ± 0.418
2.75AspPhe: 2.75 ± 0.442
7.715AspGly: 7.715 ± 1.728
1.833AspHis: 1.833 ± 0.51
3.514AspIle: 3.514 ± 0.531
4.431AspLys: 4.431 ± 0.545
5.194AspLeu: 5.194 ± 0.67
1.986AspMet: 1.986 ± 0.353
2.826AspAsn: 2.826 ± 0.507
3.819AspPro: 3.819 ± 0.508
2.674AspGln: 2.674 ± 0.407
3.667AspArg: 3.667 ± 0.599
4.66AspSer: 4.66 ± 0.562
5.194AspThr: 5.194 ± 0.722
3.667AspVal: 3.667 ± 0.44
1.451AspTrp: 1.451 ± 0.332
3.514AspTyr: 3.514 ± 0.553
0.0AspXaa: 0.0 ± 0.0
Glu
4.583GluAla: 4.583 ± 0.743
0.076GluCys: 0.076 ± 0.082
2.903GluAsp: 2.903 ± 0.503
3.59GluGlu: 3.59 ± 0.77
1.757GluPhe: 1.757 ± 0.325
3.437GluGly: 3.437 ± 0.438
1.146GluHis: 1.146 ± 0.28
3.437GluIle: 3.437 ± 0.459
4.201GluLys: 4.201 ± 0.801
5.882GluLeu: 5.882 ± 0.607
1.681GluMet: 1.681 ± 0.394
2.139GluAsn: 2.139 ± 0.35
1.528GluPro: 1.528 ± 0.337
3.132GluGln: 3.132 ± 0.572
2.597GluArg: 2.597 ± 0.469
2.292GluSer: 2.292 ± 0.351
3.285GluThr: 3.285 ± 0.457
3.743GluVal: 3.743 ± 0.573
0.993GluTrp: 0.993 ± 0.289
2.368GluTyr: 2.368 ± 0.508
0.0GluXaa: 0.0 ± 0.0
Phe
2.597PheAla: 2.597 ± 0.459
0.229PheCys: 0.229 ± 0.178
2.292PheAsp: 2.292 ± 0.403
1.833PheGlu: 1.833 ± 0.414
0.687PhePhe: 0.687 ± 0.233
2.903PheGly: 2.903 ± 0.434
0.687PheHis: 0.687 ± 0.347
1.986PheIle: 1.986 ± 0.581
2.75PheLys: 2.75 ± 0.352
3.132PheLeu: 3.132 ± 0.668
1.222PheMet: 1.222 ± 0.256
1.91PheAsn: 1.91 ± 0.472
1.069PhePro: 1.069 ± 0.261
1.528PheGln: 1.528 ± 0.397
1.299PheArg: 1.299 ± 0.414
1.833PheSer: 1.833 ± 0.408
3.132PheThr: 3.132 ± 0.656
1.833PheVal: 1.833 ± 0.367
0.458PheTrp: 0.458 ± 0.173
0.917PheTyr: 0.917 ± 0.283
0.0PheXaa: 0.0 ± 0.0
Gly
5.424GlyAla: 5.424 ± 0.998
0.382GlyCys: 0.382 ± 0.21
4.66GlyAsp: 4.66 ± 0.731
3.819GlyGlu: 3.819 ± 0.508
2.75GlyPhe: 2.75 ± 0.421
3.743GlyGly: 3.743 ± 0.627
1.069GlyHis: 1.069 ± 0.248
5.653GlyIle: 5.653 ± 0.665
5.882GlyLys: 5.882 ± 0.618
4.201GlyLeu: 4.201 ± 0.584
2.521GlyMet: 2.521 ± 0.366
2.903GlyAsn: 2.903 ± 0.492
1.757GlyPro: 1.757 ± 0.412
1.757GlyGln: 1.757 ± 0.37
3.132GlyArg: 3.132 ± 0.523
4.583GlySer: 4.583 ± 0.613
5.5GlyThr: 5.5 ± 0.816
4.66GlyVal: 4.66 ± 0.697
1.451GlyTrp: 1.451 ± 0.294
2.979GlyTyr: 2.979 ± 0.47
0.0GlyXaa: 0.0 ± 0.0
His
1.91HisAla: 1.91 ± 0.415
0.0HisCys: 0.0 ± 0.0
1.528HisAsp: 1.528 ± 0.299
0.687HisGlu: 0.687 ± 0.225
0.458HisPhe: 0.458 ± 0.186
1.146HisGly: 1.146 ± 0.248
0.382HisHis: 0.382 ± 0.186
1.299HisIle: 1.299 ± 0.361
0.84HisLys: 0.84 ± 0.264
1.528HisLeu: 1.528 ± 0.292
0.153HisMet: 0.153 ± 0.113
0.306HisAsn: 0.306 ± 0.168
0.993HisPro: 0.993 ± 0.266
0.687HisGln: 0.687 ± 0.201
0.764HisArg: 0.764 ± 0.329
1.222HisSer: 1.222 ± 0.419
1.146HisThr: 1.146 ± 0.333
1.069HisVal: 1.069 ± 0.335
0.382HisTrp: 0.382 ± 0.186
0.917HisTyr: 0.917 ± 0.293
0.0HisXaa: 0.0 ± 0.0
Ile
4.889IleAla: 4.889 ± 0.491
0.382IleCys: 0.382 ± 0.158
5.118IleAsp: 5.118 ± 0.597
4.583IleGlu: 4.583 ± 0.697
1.222IlePhe: 1.222 ± 0.259
4.125IleGly: 4.125 ± 0.526
1.146IleHis: 1.146 ± 0.276
3.437IleIle: 3.437 ± 0.492
5.424IleLys: 5.424 ± 0.683
3.59IleLeu: 3.59 ± 0.554
1.375IleMet: 1.375 ± 0.353
3.59IleAsn: 3.59 ± 0.439
2.292IlePro: 2.292 ± 0.305
1.681IleGln: 1.681 ± 0.389
2.444IleArg: 2.444 ± 0.396
4.965IleSer: 4.965 ± 0.644
4.812IleThr: 4.812 ± 0.512
3.208IleVal: 3.208 ± 0.384
0.764IleTrp: 0.764 ± 0.265
1.528IleTyr: 1.528 ± 0.3
0.0IleXaa: 0.0 ± 0.0
Lys
6.569LysAla: 6.569 ± 0.894
0.306LysCys: 0.306 ± 0.208
5.271LysAsp: 5.271 ± 0.575
4.049LysGlu: 4.049 ± 0.658
2.75LysPhe: 2.75 ± 0.46
4.201LysGly: 4.201 ± 0.956
1.222LysHis: 1.222 ± 0.293
3.972LysIle: 3.972 ± 0.588
5.042LysLys: 5.042 ± 0.698
6.493LysLeu: 6.493 ± 0.788
1.986LysMet: 1.986 ± 0.397
3.896LysAsn: 3.896 ± 0.498
3.514LysPro: 3.514 ± 0.546
4.354LysGln: 4.354 ± 0.643
4.354LysArg: 4.354 ± 0.572
4.812LysSer: 4.812 ± 0.397
4.66LysThr: 4.66 ± 0.549
3.361LysVal: 3.361 ± 0.456
1.069LysTrp: 1.069 ± 0.227
2.139LysTyr: 2.139 ± 0.409
0.0LysXaa: 0.0 ± 0.0
Leu
7.333LeuAla: 7.333 ± 0.777
0.84LeuCys: 0.84 ± 0.307
5.958LeuAsp: 5.958 ± 0.686
3.667LeuGlu: 3.667 ± 0.501
2.674LeuPhe: 2.674 ± 0.509
4.507LeuGly: 4.507 ± 0.679
1.451LeuHis: 1.451 ± 0.356
4.812LeuIle: 4.812 ± 0.485
6.799LeuLys: 6.799 ± 0.921
5.653LeuLeu: 5.653 ± 0.562
1.604LeuMet: 1.604 ± 0.386
3.285LeuAsn: 3.285 ± 0.439
3.743LeuPro: 3.743 ± 0.478
3.361LeuGln: 3.361 ± 0.441
4.354LeuArg: 4.354 ± 0.523
6.264LeuSer: 6.264 ± 0.909
5.806LeuThr: 5.806 ± 0.596
4.049LeuVal: 4.049 ± 0.56
0.993LeuTrp: 0.993 ± 0.282
2.215LeuTyr: 2.215 ± 0.503
0.0LeuXaa: 0.0 ± 0.0
Met
2.597MetAla: 2.597 ± 0.402
0.0MetCys: 0.0 ± 0.0
1.299MetAsp: 1.299 ± 0.335
0.84MetGlu: 0.84 ± 0.257
0.764MetPhe: 0.764 ± 0.23
1.451MetGly: 1.451 ± 0.367
0.306MetHis: 0.306 ± 0.117
1.833MetIle: 1.833 ± 0.413
2.368MetLys: 2.368 ± 0.368
1.604MetLeu: 1.604 ± 0.291
0.687MetMet: 0.687 ± 0.256
1.299MetAsn: 1.299 ± 0.321
1.146MetPro: 1.146 ± 0.308
1.528MetGln: 1.528 ± 0.32
1.451MetArg: 1.451 ± 0.316
2.139MetSer: 2.139 ± 0.458
2.444MetThr: 2.444 ± 0.388
1.451MetVal: 1.451 ± 0.341
0.153MetTrp: 0.153 ± 0.119
0.687MetTyr: 0.687 ± 0.251
0.0MetXaa: 0.0 ± 0.0
Asn
4.812AsnAla: 4.812 ± 0.738
0.076AsnCys: 0.076 ± 0.083
3.896AsnAsp: 3.896 ± 0.525
3.056AsnGlu: 3.056 ± 0.476
1.757AsnPhe: 1.757 ± 0.419
4.66AsnGly: 4.66 ± 0.526
0.993AsnHis: 0.993 ± 0.257
2.597AsnIle: 2.597 ± 0.394
3.437AsnLys: 3.437 ± 0.423
3.437AsnLeu: 3.437 ± 0.4
1.604AsnMet: 1.604 ± 0.303
2.368AsnAsn: 2.368 ± 0.304
2.139AsnPro: 2.139 ± 0.362
1.833AsnGln: 1.833 ± 0.352
2.75AsnArg: 2.75 ± 0.343
3.056AsnSer: 3.056 ± 0.655
2.292AsnThr: 2.292 ± 0.392
2.597AsnVal: 2.597 ± 0.376
0.535AsnTrp: 0.535 ± 0.236
1.528AsnTyr: 1.528 ± 0.381
0.0AsnXaa: 0.0 ± 0.0
Pro
2.826ProAla: 2.826 ± 0.401
0.076ProCys: 0.076 ± 0.071
2.597ProAsp: 2.597 ± 0.595
3.285ProGlu: 3.285 ± 0.536
1.146ProPhe: 1.146 ± 0.242
1.681ProGly: 1.681 ± 0.393
0.764ProHis: 0.764 ± 0.283
2.215ProIle: 2.215 ± 0.386
3.667ProLys: 3.667 ± 0.587
3.056ProLeu: 3.056 ± 0.533
0.993ProMet: 0.993 ± 0.435
1.451ProAsn: 1.451 ± 0.378
0.764ProPro: 0.764 ± 0.371
1.681ProGln: 1.681 ± 0.343
0.917ProArg: 0.917 ± 0.234
3.208ProSer: 3.208 ± 0.33
2.139ProThr: 2.139 ± 0.381
2.903ProVal: 2.903 ± 0.486
0.535ProTrp: 0.535 ± 0.217
1.146ProTyr: 1.146 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
5.271GlnAla: 5.271 ± 0.78
0.229GlnCys: 0.229 ± 0.158
1.757GlnAsp: 1.757 ± 0.432
2.062GlnGlu: 2.062 ± 0.334
1.91GlnPhe: 1.91 ± 0.335
2.444GlnGly: 2.444 ± 0.408
1.222GlnHis: 1.222 ± 0.283
3.437GlnIle: 3.437 ± 0.459
2.75GlnLys: 2.75 ± 0.468
4.125GlnLeu: 4.125 ± 0.534
1.146GlnMet: 1.146 ± 0.368
1.681GlnAsn: 1.681 ± 0.318
1.375GlnPro: 1.375 ± 0.302
2.674GlnGln: 2.674 ± 0.513
1.833GlnArg: 1.833 ± 0.359
1.986GlnSer: 1.986 ± 0.385
2.368GlnThr: 2.368 ± 0.355
2.215GlnVal: 2.215 ± 0.379
0.84GlnTrp: 0.84 ± 0.227
2.215GlnTyr: 2.215 ± 0.418
0.0GlnXaa: 0.0 ± 0.0
Arg
3.59ArgAla: 3.59 ± 0.566
0.229ArgCys: 0.229 ± 0.176
3.437ArgAsp: 3.437 ± 0.624
3.056ArgGlu: 3.056 ± 0.472
1.757ArgPhe: 1.757 ± 0.323
2.597ArgGly: 2.597 ± 0.447
0.84ArgHis: 0.84 ± 0.317
2.674ArgIle: 2.674 ± 0.468
2.826ArgLys: 2.826 ± 0.444
4.049ArgLeu: 4.049 ± 0.423
1.375ArgMet: 1.375 ± 0.286
1.757ArgAsn: 1.757 ± 0.353
1.146ArgPro: 1.146 ± 0.273
3.056ArgGln: 3.056 ± 0.526
1.757ArgArg: 1.757 ± 0.378
2.444ArgSer: 2.444 ± 0.375
2.903ArgThr: 2.903 ± 0.332
2.979ArgVal: 2.979 ± 0.503
0.535ArgTrp: 0.535 ± 0.18
1.681ArgTyr: 1.681 ± 0.409
0.0ArgXaa: 0.0 ± 0.0
Ser
4.201SerAla: 4.201 ± 0.603
0.229SerCys: 0.229 ± 0.128
5.576SerAsp: 5.576 ± 0.856
2.521SerGlu: 2.521 ± 0.459
2.139SerPhe: 2.139 ± 0.37
6.493SerGly: 6.493 ± 0.828
0.764SerHis: 0.764 ± 0.34
3.59SerIle: 3.59 ± 0.39
3.896SerLys: 3.896 ± 0.747
5.5SerLeu: 5.5 ± 0.571
1.451SerMet: 1.451 ± 0.32
4.049SerAsn: 4.049 ± 0.544
2.215SerPro: 2.215 ± 0.532
3.437SerGln: 3.437 ± 0.586
2.826SerArg: 2.826 ± 0.387
4.431SerSer: 4.431 ± 0.629
4.278SerThr: 4.278 ± 0.565
4.66SerVal: 4.66 ± 0.684
1.222SerTrp: 1.222 ± 0.326
2.75SerTyr: 2.75 ± 0.56
0.0SerXaa: 0.0 ± 0.0
Thr
7.41ThrAla: 7.41 ± 1.269
0.229ThrCys: 0.229 ± 0.131
5.347ThrAsp: 5.347 ± 0.681
3.132ThrGlu: 3.132 ± 0.409
2.062ThrPhe: 2.062 ± 0.419
4.507ThrGly: 4.507 ± 0.56
0.611ThrHis: 0.611 ± 0.386
4.736ThrIle: 4.736 ± 0.607
4.201ThrLys: 4.201 ± 0.429
5.958ThrLeu: 5.958 ± 0.638
0.917ThrMet: 0.917 ± 0.241
3.743ThrAsn: 3.743 ± 0.59
3.514ThrPro: 3.514 ± 0.512
1.986ThrGln: 1.986 ± 0.337
2.75ThrArg: 2.75 ± 0.504
4.201ThrSer: 4.201 ± 0.517
4.278ThrThr: 4.278 ± 0.557
4.583ThrVal: 4.583 ± 0.736
0.535ThrTrp: 0.535 ± 0.203
2.75ThrTyr: 2.75 ± 0.439
0.0ThrXaa: 0.0 ± 0.0
Val
4.583ValAla: 4.583 ± 0.598
0.306ValCys: 0.306 ± 0.162
5.576ValAsp: 5.576 ± 0.737
3.056ValGlu: 3.056 ± 0.619
1.528ValPhe: 1.528 ± 0.352
4.049ValGly: 4.049 ± 0.419
0.535ValHis: 0.535 ± 0.19
3.59ValIle: 3.59 ± 0.381
4.965ValLys: 4.965 ± 0.678
4.507ValLeu: 4.507 ± 0.469
1.451ValMet: 1.451 ± 0.273
4.278ValAsn: 4.278 ± 0.599
2.215ValPro: 2.215 ± 0.492
1.757ValGln: 1.757 ± 0.331
2.215ValArg: 2.215 ± 0.344
4.507ValSer: 4.507 ± 0.589
4.507ValThr: 4.507 ± 0.55
3.743ValVal: 3.743 ± 0.506
0.84ValTrp: 0.84 ± 0.261
1.528ValTyr: 1.528 ± 0.374
0.0ValXaa: 0.0 ± 0.0
Trp
0.917TrpAla: 0.917 ± 0.353
0.153TrpCys: 0.153 ± 0.116
0.764TrpAsp: 0.764 ± 0.266
0.687TrpGlu: 0.687 ± 0.219
0.458TrpPhe: 0.458 ± 0.205
0.611TrpGly: 0.611 ± 0.263
0.458TrpHis: 0.458 ± 0.203
0.993TrpIle: 0.993 ± 0.298
0.84TrpLys: 0.84 ± 0.26
1.451TrpLeu: 1.451 ± 0.364
0.076TrpMet: 0.076 ± 0.077
1.222TrpAsn: 1.222 ± 0.454
0.229TrpPro: 0.229 ± 0.135
0.687TrpGln: 0.687 ± 0.218
0.84TrpArg: 0.84 ± 0.344
0.993TrpSer: 0.993 ± 0.369
0.993TrpThr: 0.993 ± 0.269
1.069TrpVal: 1.069 ± 0.348
0.382TrpTrp: 0.382 ± 0.214
0.687TrpTyr: 0.687 ± 0.269
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.056TyrAla: 3.056 ± 0.599
0.153TyrCys: 0.153 ± 0.11
3.132TyrAsp: 3.132 ± 0.448
1.833TyrGlu: 1.833 ± 0.513
2.139TyrPhe: 2.139 ± 0.369
2.139TyrGly: 2.139 ± 0.376
0.611TyrHis: 0.611 ± 0.211
2.062TyrIle: 2.062 ± 0.602
2.368TyrLys: 2.368 ± 0.487
2.903TyrLeu: 2.903 ± 0.656
0.458TyrMet: 0.458 ± 0.223
1.604TyrAsn: 1.604 ± 0.391
1.681TyrPro: 1.681 ± 0.361
2.062TyrGln: 2.062 ± 0.338
1.681TyrArg: 1.681 ± 0.33
2.903TyrSer: 2.903 ± 0.5
2.521TyrThr: 2.521 ± 0.484
1.833TyrVal: 1.833 ± 0.421
0.535TyrTrp: 0.535 ± 0.221
1.299TyrTyr: 1.299 ± 0.436
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13092 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski