Spider monkey simian foamy virus
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8HC81|A8HC81_9RETR Bel2 OS=Spider monkey simian foamy virus OX=2170200 GN=bel2 PE=4 SV=1
MM1 pKa = 8.15 ASQQEE6 pKa = 4.43 KK7 pKa = 10.78 NSIVPEE13 pKa = 4.24 DD14 pKa = 3.95 DD15 pKa = 3.54 FMEE18 pKa = 4.37 EE19 pKa = 3.54 LRR21 pKa = 11.84 AFLAEE26 pKa = 4.37 FPIAEE31 pKa = 4.38 QPLEE35 pKa = 4.41 DD36 pKa = 5.61 DD37 pKa = 3.88 VFQAEE42 pKa = 4.74 GQQPSQFLEE51 pKa = 4.48 DD52 pKa = 4.06 PGEE55 pKa = 4.57 GPSGASAQAGASSGPPKK72 pKa = 10.73 VSGKK76 pKa = 9.0 LWNMMAHH83 pKa = 6.44 HH84 pKa = 7.42 PNRR87 pKa = 11.84 GTTSHH92 pKa = 6.64 GPDD95 pKa = 3.42 EE96 pKa = 4.97 PPTGRR101 pKa = 11.84 EE102 pKa = 3.42 PRR104 pKa = 11.84 LRR106 pKa = 11.84 WLQEE110 pKa = 3.26 ARR112 pKa = 11.84 MEE114 pKa = 3.93 TAYY117 pKa = 9.87 IRR119 pKa = 11.84 FEE121 pKa = 3.97 KK122 pKa = 10.61 DD123 pKa = 2.78 LEE125 pKa = 4.5 KK126 pKa = 10.84 FFPTPEE132 pKa = 3.61 QRR134 pKa = 11.84 EE135 pKa = 4.04 FFIQRR140 pKa = 11.84 AIITAAGYY148 pKa = 10.43 KK149 pKa = 9.45 PDD151 pKa = 3.6 LAIYY155 pKa = 7.3 TAHH158 pKa = 6.51 NGWYY162 pKa = 10.38 ACIQPHH168 pKa = 5.94 TGDD171 pKa = 3.59 LGEE174 pKa = 4.63 KK175 pKa = 9.65 YY176 pKa = 9.56 EE177 pKa = 4.62 VYY179 pKa = 10.5 YY180 pKa = 10.98 KK181 pKa = 10.57 CLKK184 pKa = 10.09 CGNEE188 pKa = 3.63 QWDD191 pKa = 3.91 PLLYY195 pKa = 10.68 DD196 pKa = 3.91 WDD198 pKa = 4.3 PSVLMFLRR206 pKa = 11.84 AWWKK210 pKa = 8.46 TPHH213 pKa = 6.31 TSSPLTAMRR222 pKa = 11.84 RR223 pKa = 11.84 HH224 pKa = 6.44 DD225 pKa = 3.95 AVCAGIYY232 pKa = 9.82 SDD234 pKa = 4.52 SSSSTEE240 pKa = 3.66 PPKK243 pKa = 10.36 RR244 pKa = 11.84 RR245 pKa = 11.84 QRR247 pKa = 11.84 GDD249 pKa = 3.15 PGHH252 pKa = 6.75 RR253 pKa = 11.84 WGAYY257 pKa = 8.88 KK258 pKa = 9.59 RR259 pKa = 11.84 TFDD262 pKa = 4.85 SGPLEE267 pKa = 4.04 QLVLTHH273 pKa = 6.2 TCAYY277 pKa = 5.07 TTEE280 pKa = 4.0 WPEE283 pKa = 3.76 PKK285 pKa = 9.65 RR286 pKa = 11.84 IHH288 pKa = 6.82 LGDD291 pKa = 4.46 LEE293 pKa = 4.87 TVCPALPDD301 pKa = 4.27 PGEE304 pKa = 4.46 GGTNSEE310 pKa = 5.07 MEE312 pKa = 4.01 PQYY315 pKa = 11.09 QQGSWTFDD323 pKa = 3.03 QQ324 pKa = 4.84
Molecular weight: 36.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.908
IPC2_protein 4.965
IPC_protein 4.914
Toseland 4.838
ProMoST 5.041
Dawson 4.889
Bjellqvist 5.016
Wikipedia 4.749
Rodwell 4.813
Grimsley 4.762
Solomon 4.889
Lehninger 4.851
Nozaki 5.003
DTASelect 5.143
Thurlkill 4.838
EMBOSS 4.787
Sillero 5.08
Patrickios 3.808
IPC_peptide 4.902
IPC2_peptide 5.08
IPC2.peptide.svr19 5.024
Protein with the highest isoelectric point:
>tr|A8HC78|A8HC78_9RETR Pr125Pol OS=Spider monkey simian foamy virus OX=2170200 GN=pol PE=4 SV=1
MM1 pKa = 7.33 AQGNQPEE8 pKa = 4.94 HH9 pKa = 7.77 IDD11 pKa = 3.55 IGHH14 pKa = 6.22 LTILLRR20 pKa = 11.84 EE21 pKa = 4.38 NGLPTNPPHH30 pKa = 5.13 GTEE33 pKa = 3.86 YY34 pKa = 10.71 AVRR37 pKa = 11.84 MTEE40 pKa = 4.31 GWWGDD45 pKa = 3.46 YY46 pKa = 11.19 ARR48 pKa = 11.84 FTVIRR53 pKa = 11.84 VAFQDD58 pKa = 3.6 NQGNPLPRR66 pKa = 11.84 PEE68 pKa = 3.87 WEE70 pKa = 4.2 YY71 pKa = 10.47 INRR74 pKa = 11.84 DD75 pKa = 3.33 VRR77 pKa = 11.84 PLNDD81 pKa = 4.26 SIIGCTFRR89 pKa = 11.84 QAQQAFNNIDD99 pKa = 3.77 LARR102 pKa = 11.84 TPSRR106 pKa = 11.84 YY107 pKa = 10.02 GPLSNGLFLITDD119 pKa = 4.29 PEE121 pKa = 4.28 WLTFNPLSAVEE132 pKa = 4.63 IADD135 pKa = 5.39 LDD137 pKa = 3.87 DD138 pKa = 3.94 QEE140 pKa = 5.27 IRR142 pKa = 11.84 DD143 pKa = 4.3 FLAHH147 pKa = 5.87 ATIVLDD153 pKa = 3.61 NVMADD158 pKa = 3.19 VVIQQRR164 pKa = 11.84 EE165 pKa = 4.15 IEE167 pKa = 4.94 DD168 pKa = 3.47 LTKK171 pKa = 10.83 TNLRR175 pKa = 11.84 LRR177 pKa = 11.84 NALASSGTYY186 pKa = 9.48 QPIGMPIAQSSPAGPSASVAGPHH209 pKa = 5.64 MPAMPSVPYY218 pKa = 10.48 ISAIPSAPPAPIPVAPAAAPPLGPLPSAPAPPVLPVIQPNTFGTDD263 pKa = 3.24 GNGYY267 pKa = 7.18 TAVGPVPVLPIAQIKK282 pKa = 9.55 AVIGEE287 pKa = 4.34 APTDD291 pKa = 3.44 ARR293 pKa = 11.84 QIPLWVAKK301 pKa = 9.76 HH302 pKa = 5.27 AAAIEE307 pKa = 4.52 GTFPTGSADD316 pKa = 3.29 VRR318 pKa = 11.84 CRR320 pKa = 11.84 VLNALLTSHH329 pKa = 6.74 GGMTLSPNEE338 pKa = 4.21 CGTWSLAAAALYY350 pKa = 9.59 QRR352 pKa = 11.84 IYY354 pKa = 11.23 GVIPLHH360 pKa = 6.64 DD361 pKa = 5.1 LPHH364 pKa = 6.12 TMAEE368 pKa = 4.07 VARR371 pKa = 11.84 RR372 pKa = 11.84 EE373 pKa = 4.41 GILVAFNMGMTFTNNSFDD391 pKa = 4.06 VVWGIIRR398 pKa = 11.84 PLLPGQASVAMLQGYY413 pKa = 9.51 LDD415 pKa = 3.76 QYY417 pKa = 11.18 QGQQQKK423 pKa = 10.29 AQAFPTLLRR432 pKa = 11.84 RR433 pKa = 11.84 TFEE436 pKa = 4.37 TLGLNYY442 pKa = 9.94 LGQSIRR448 pKa = 11.84 SNQPARR454 pKa = 11.84 TSGSVAGSITQGRR467 pKa = 11.84 GRR469 pKa = 11.84 GQPRR473 pKa = 11.84 SRR475 pKa = 11.84 PPNRR479 pKa = 11.84 ASNRR483 pKa = 11.84 SNNPPRR489 pKa = 11.84 PPPPRR494 pKa = 11.84 SQEE497 pKa = 4.07 QQPSPAGRR505 pKa = 11.84 GSSSSSAPPQRR516 pKa = 11.84 SGYY519 pKa = 8.17 NLRR522 pKa = 11.84 QQINRR527 pKa = 11.84 PQRR530 pKa = 11.84 LGGGPNPYY538 pKa = 10.18 RR539 pKa = 11.84 NLEE542 pKa = 3.82 NRR544 pKa = 11.84 NIPRR548 pKa = 11.84 SEE550 pKa = 4.08 TVPSSRR556 pKa = 11.84 RR557 pKa = 11.84 AEE559 pKa = 3.93 TSEE562 pKa = 4.02 TNRR565 pKa = 11.84 PNQARR570 pKa = 11.84 GGQQGSSNRR579 pKa = 11.84 NQTRR583 pKa = 11.84 SINTVRR589 pKa = 11.84 ASDD592 pKa = 3.75 SQVIPSAPPAEE603 pKa = 4.35 TNPAATAVTPNGPNQHH619 pKa = 6.39 SGRR622 pKa = 11.84 NN623 pKa = 3.57
Molecular weight: 67.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.9
IPC2_protein 9.107
IPC_protein 9.838
Toseland 9.326
ProMoST 9.428
Dawson 9.736
Bjellqvist 9.633
Wikipedia 10.043
Rodwell 9.721
Grimsley 9.882
Solomon 9.809
Lehninger 9.736
Nozaki 9.472
DTASelect 9.589
Thurlkill 9.545
EMBOSS 9.809
Sillero 9.692
Patrickios 4.863
IPC_peptide 9.78
IPC2_peptide 8.799
IPC2.peptide.svr19 8.054
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3349
268
1148
669.8
75.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.271 ± 0.943
1.583 ± 0.699
4.598 ± 0.405
5.524 ± 0.777
2.717 ± 0.262
5.673 ± 0.698
2.448 ± 0.242
6.659 ± 0.645
5.464 ± 1.248
9.645 ± 0.81
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.732 ± 0.256
5.046 ± 0.668
7.943 ± 1.037
5.345 ± 0.32
4.897 ± 0.727
6.42 ± 0.445
7.077 ± 0.344
5.255 ± 0.537
2.21 ± 0.346
3.494 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here