Saimiri sciureus papillomavirus 3
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5QK84|W5QK84_9PAPI Minor capsid protein L2 OS=Saimiri sciureus papillomavirus 3 OX=990306 GN=L2 PE=3 SV=1
MM1 pKa = 7.38 IGAAPTLKK9 pKa = 10.6 DD10 pKa = 3.67 IILSEE15 pKa = 4.26 LPEE18 pKa = 4.32 PVDD21 pKa = 4.04 LRR23 pKa = 11.84 CHH25 pKa = 6.25 EE26 pKa = 5.34 DD27 pKa = 3.0 INYY30 pKa = 10.76 DD31 pKa = 3.64 EE32 pKa = 4.76 VDD34 pKa = 3.69 SNEE37 pKa = 4.28 DD38 pKa = 3.09 VTEE41 pKa = 4.04 GHH43 pKa = 6.21 TGLYY47 pKa = 9.7 QVVCQCNTCSQSLRR61 pKa = 11.84 LLVKK65 pKa = 9.82 CTEE68 pKa = 4.55 DD69 pKa = 3.63 DD70 pKa = 4.12 VEE72 pKa = 4.37 VLHH75 pKa = 7.45 DD76 pKa = 4.85 LLTTTLEE83 pKa = 4.36 LVCPLCARR91 pKa = 11.84 EE92 pKa = 4.04 MNN94 pKa = 3.85
Molecular weight: 10.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.913
IPC2_protein 4.088
IPC_protein 3.999
Toseland 3.821
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.884
Rodwell 3.846
Grimsley 3.732
Solomon 3.961
Lehninger 3.923
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.126
Patrickios 0.477
IPC_peptide 3.961
IPC2_peptide 4.101
IPC2.peptide.svr19 4.008
Protein with the highest isoelectric point:
>tr|W5QK96|W5QK96_9PAPI Major capsid protein L1 OS=Saimiri sciureus papillomavirus 3 OX=990306 GN=L1 PE=3 SV=1
MM1 pKa = 8.02 EE2 pKa = 5.19 YY3 pKa = 8.09 PTSIPDD9 pKa = 3.29 LCSEE13 pKa = 3.89 VDD15 pKa = 3.27 IVPWHH20 pKa = 6.35 LQLTCIFCKK29 pKa = 10.4 CHH31 pKa = 7.26 LDD33 pKa = 4.21 VGDD36 pKa = 4.16 LCAFIHH42 pKa = 7.16 NDD44 pKa = 5.17 LKK46 pKa = 11.33 LKK48 pKa = 9.36 WIKK51 pKa = 10.35 SYY53 pKa = 10.48 PYY55 pKa = 9.7 AACHH59 pKa = 6.18 RR60 pKa = 11.84 CILLKK65 pKa = 10.81 ARR67 pKa = 11.84 VLAWRR72 pKa = 11.84 YY73 pKa = 5.45 QTEE76 pKa = 3.63 AAYY79 pKa = 10.59 ARR81 pKa = 11.84 TVEE84 pKa = 4.31 HH85 pKa = 7.68 DD86 pKa = 3.54 CGQPLGNIRR95 pKa = 11.84 MRR97 pKa = 11.84 CIVCTALLTSEE108 pKa = 4.58 DD109 pKa = 3.85 KK110 pKa = 10.53 IRR112 pKa = 11.84 HH113 pKa = 5.55 IEE115 pKa = 3.58 HH116 pKa = 6.41 HH117 pKa = 6.22 RR118 pKa = 11.84 RR119 pKa = 11.84 FVKK122 pKa = 10.5 AAGYY126 pKa = 8.33 WRR128 pKa = 11.84 GRR130 pKa = 11.84 CYY132 pKa = 10.74 HH133 pKa = 6.73 CWNTALL139 pKa = 4.6
Molecular weight: 16.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.148
IPC2_protein 7.585
IPC_protein 7.41
Toseland 6.971
ProMoST 7.995
Dawson 8.083
Bjellqvist 8.609
Wikipedia 7.98
Rodwell 8.097
Grimsley 7.0
Solomon 8.141
Lehninger 8.17
Nozaki 8.931
DTASelect 8.244
Thurlkill 8.287
EMBOSS 8.302
Sillero 8.668
Patrickios 2.257
IPC_peptide 8.141
IPC2_peptide 8.141
IPC2.peptide.svr19 8.005
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2278
94
647
379.7
42.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.263 ± 0.744
2.809 ± 0.756
5.926 ± 0.208
5.224 ± 0.789
3.6 ± 0.612
6.19 ± 0.347
2.502 ± 0.623
3.731 ± 0.409
4.478 ± 0.676
7.946 ± 0.596
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.8 ± 0.304
3.205 ± 0.701
6.936 ± 1.054
4.302 ± 0.532
5.751 ± 0.35
7.463 ± 0.876
6.892 ± 0.506
6.673 ± 0.753
1.8 ± 0.457
3.512 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here