Amino acid dipepetide frequency for Flavobacterium phage Fpv1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.038AlaAla: 3.038 ± 0.357
0.371AlaCys: 0.371 ± 0.139
2.594AlaAsp: 2.594 ± 0.319
3.816AlaGlu: 3.816 ± 0.489
1.63AlaPhe: 1.63 ± 0.226
3.816AlaGly: 3.816 ± 0.486
0.704AlaHis: 0.704 ± 0.18
3.631AlaIle: 3.631 ± 0.372
4.78AlaLys: 4.78 ± 0.71
4.039AlaLeu: 4.039 ± 0.471
1.519AlaMet: 1.519 ± 0.217
3.223AlaAsn: 3.223 ± 0.538
1.334AlaPro: 1.334 ± 0.248
1.927AlaGln: 1.927 ± 0.387
1.556AlaArg: 1.556 ± 0.292
2.816AlaSer: 2.816 ± 0.295
2.816AlaThr: 2.816 ± 0.429
2.668AlaVal: 2.668 ± 0.508
0.408AlaTrp: 0.408 ± 0.153
2.038AlaTyr: 2.038 ± 0.311
0.0AlaXaa: 0.0 ± 0.0
Cys
0.259CysAla: 0.259 ± 0.105
0.185CysCys: 0.185 ± 0.087
0.556CysAsp: 0.556 ± 0.198
0.704CysGlu: 0.704 ± 0.228
0.259CysPhe: 0.259 ± 0.12
0.741CysGly: 0.741 ± 0.233
0.148CysHis: 0.148 ± 0.078
0.667CysIle: 0.667 ± 0.237
1.149CysLys: 1.149 ± 0.346
0.815CysLeu: 0.815 ± 0.219
0.074CysMet: 0.074 ± 0.055
0.667CysAsn: 0.667 ± 0.185
0.408CysPro: 0.408 ± 0.124
0.185CysGln: 0.185 ± 0.087
0.111CysArg: 0.111 ± 0.062
0.333CysSer: 0.333 ± 0.127
0.63CysThr: 0.63 ± 0.197
0.593CysVal: 0.593 ± 0.189
0.074CysTrp: 0.074 ± 0.068
0.556CysTyr: 0.556 ± 0.204
0.0CysXaa: 0.0 ± 0.0
Asp
2.853AspAla: 2.853 ± 0.506
0.926AspCys: 0.926 ± 0.304
3.594AspAsp: 3.594 ± 0.351
5.15AspGlu: 5.15 ± 0.557
3.742AspPhe: 3.742 ± 0.282
3.372AspGly: 3.372 ± 0.415
0.556AspHis: 0.556 ± 0.171
5.002AspIle: 5.002 ± 0.426
5.669AspLys: 5.669 ± 0.403
5.002AspLeu: 5.002 ± 0.441
1.704AspMet: 1.704 ± 0.264
4.15AspAsn: 4.15 ± 0.367
0.815AspPro: 0.815 ± 0.192
0.741AspGln: 0.741 ± 0.159
1.778AspArg: 1.778 ± 0.264
4.113AspSer: 4.113 ± 0.384
3.186AspThr: 3.186 ± 0.402
3.335AspVal: 3.335 ± 0.398
0.741AspTrp: 0.741 ± 0.195
3.26AspTyr: 3.26 ± 0.292
0.0AspXaa: 0.0 ± 0.0
Glu
4.446GluAla: 4.446 ± 0.453
0.408GluCys: 0.408 ± 0.144
3.816GluAsp: 3.816 ± 0.364
7.04GluGlu: 7.04 ± 0.593
3.409GluPhe: 3.409 ± 0.388
3.779GluGly: 3.779 ± 0.495
1.149GluHis: 1.149 ± 0.18
5.854GluIle: 5.854 ± 0.484
7.484GluLys: 7.484 ± 0.847
7.114GluLeu: 7.114 ± 0.595
1.853GluMet: 1.853 ± 0.283
6.225GluAsn: 6.225 ± 0.73
1.519GluPro: 1.519 ± 0.246
3.853GluGln: 3.853 ± 0.366
2.26GluArg: 2.26 ± 0.385
3.705GluSer: 3.705 ± 0.457
4.483GluThr: 4.483 ± 0.508
4.78GluVal: 4.78 ± 0.393
1.037GluTrp: 1.037 ± 0.256
3.26GluTyr: 3.26 ± 0.315
0.0GluXaa: 0.0 ± 0.0
Phe
2.149PheAla: 2.149 ± 0.307
0.333PheCys: 0.333 ± 0.141
3.372PheAsp: 3.372 ± 0.35
3.779PheGlu: 3.779 ± 0.419
1.593PhePhe: 1.593 ± 0.265
2.557PheGly: 2.557 ± 0.322
0.519PheHis: 0.519 ± 0.1
4.113PheIle: 4.113 ± 0.391
4.039PheLys: 4.039 ± 0.426
2.742PheLeu: 2.742 ± 0.348
0.815PheMet: 0.815 ± 0.183
4.52PheAsn: 4.52 ± 0.544
1.371PhePro: 1.371 ± 0.282
1.741PheGln: 1.741 ± 0.266
1.741PheArg: 1.741 ± 0.315
2.334PheSer: 2.334 ± 0.295
2.705PheThr: 2.705 ± 0.376
2.816PheVal: 2.816 ± 0.312
0.371PheTrp: 0.371 ± 0.104
2.297PheTyr: 2.297 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
2.223GlyAla: 2.223 ± 0.317
0.741GlyCys: 0.741 ± 0.246
2.779GlyAsp: 2.779 ± 0.231
3.446GlyGlu: 3.446 ± 0.369
3.298GlyPhe: 3.298 ± 0.286
2.705GlyGly: 2.705 ± 0.421
0.519GlyHis: 0.519 ± 0.141
5.224GlyIle: 5.224 ± 0.453
6.113GlyLys: 6.113 ± 0.731
4.298GlyLeu: 4.298 ± 0.364
1.334GlyMet: 1.334 ± 0.246
3.446GlyAsn: 3.446 ± 0.544
0.815GlyPro: 0.815 ± 0.133
2.112GlyGln: 2.112 ± 0.295
1.667GlyArg: 1.667 ± 0.29
3.668GlySer: 3.668 ± 0.599
3.668GlyThr: 3.668 ± 0.403
3.409GlyVal: 3.409 ± 0.437
0.741GlyTrp: 0.741 ± 0.19
2.705GlyTyr: 2.705 ± 0.308
0.0GlyXaa: 0.0 ± 0.0
His
0.704HisAla: 0.704 ± 0.175
0.111HisCys: 0.111 ± 0.062
0.593HisAsp: 0.593 ± 0.139
0.519HisGlu: 0.519 ± 0.136
0.778HisPhe: 0.778 ± 0.196
0.704HisGly: 0.704 ± 0.199
0.259HisHis: 0.259 ± 0.122
1.519HisIle: 1.519 ± 0.281
1.964HisLys: 1.964 ± 0.236
1.556HisLeu: 1.556 ± 0.2
0.296HisMet: 0.296 ± 0.127
1.074HisAsn: 1.074 ± 0.215
0.556HisPro: 0.556 ± 0.122
0.815HisGln: 0.815 ± 0.151
0.704HisArg: 0.704 ± 0.169
0.667HisSer: 0.667 ± 0.145
1.445HisThr: 1.445 ± 0.29
0.445HisVal: 0.445 ± 0.137
0.259HisTrp: 0.259 ± 0.107
0.704HisTyr: 0.704 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
3.89IleAla: 3.89 ± 0.429
0.593IleCys: 0.593 ± 0.212
5.78IleAsp: 5.78 ± 0.503
7.114IleGlu: 7.114 ± 0.573
3.038IlePhe: 3.038 ± 0.444
4.076IleGly: 4.076 ± 0.475
1.63IleHis: 1.63 ± 0.369
5.15IleIle: 5.15 ± 0.632
8.373IleLys: 8.373 ± 0.489
5.595IleLeu: 5.595 ± 0.649
1.63IleMet: 1.63 ± 0.239
6.41IleAsn: 6.41 ± 0.377
2.557IlePro: 2.557 ± 0.279
3.298IleGln: 3.298 ± 0.379
1.89IleArg: 1.89 ± 0.294
5.817IleSer: 5.817 ± 0.711
5.224IleThr: 5.224 ± 0.461
3.52IleVal: 3.52 ± 0.363
0.704IleTrp: 0.704 ± 0.193
3.779IleTyr: 3.779 ± 0.413
0.0IleXaa: 0.0 ± 0.0
Lys
4.15LysAla: 4.15 ± 0.553
0.63LysCys: 0.63 ± 0.196
6.743LysAsp: 6.743 ± 0.662
8.522LysGlu: 8.522 ± 0.941
3.964LysPhe: 3.964 ± 0.445
5.484LysGly: 5.484 ± 0.539
1.741LysHis: 1.741 ± 0.293
7.892LysIle: 7.892 ± 0.546
7.558LysLys: 7.558 ± 0.543
8.003LysLeu: 8.003 ± 0.673
2.408LysMet: 2.408 ± 0.304
6.817LysAsn: 6.817 ± 0.707
2.408LysPro: 2.408 ± 0.295
3.89LysGln: 3.89 ± 0.434
2.927LysArg: 2.927 ± 0.375
5.632LysSer: 5.632 ± 0.58
5.187LysThr: 5.187 ± 0.54
5.076LysVal: 5.076 ± 0.508
0.963LysTrp: 0.963 ± 0.17
3.89LysTyr: 3.89 ± 0.345
0.0LysXaa: 0.0 ± 0.0
Leu
5.224LeuAla: 5.224 ± 0.591
0.815LeuCys: 0.815 ± 0.245
4.668LeuAsp: 4.668 ± 0.389
6.187LeuGlu: 6.187 ± 0.383
3.298LeuPhe: 3.298 ± 0.347
4.187LeuGly: 4.187 ± 0.539
1.445LeuHis: 1.445 ± 0.219
6.076LeuIle: 6.076 ± 0.533
7.632LeuLys: 7.632 ± 0.56
7.299LeuLeu: 7.299 ± 0.648
1.556LeuMet: 1.556 ± 0.309
6.447LeuAsn: 6.447 ± 0.507
3.557LeuPro: 3.557 ± 0.354
3.112LeuGln: 3.112 ± 0.275
2.149LeuArg: 2.149 ± 0.332
5.743LeuSer: 5.743 ± 0.543
5.521LeuThr: 5.521 ± 0.401
4.15LeuVal: 4.15 ± 0.437
0.556LeuTrp: 0.556 ± 0.128
3.112LeuTyr: 3.112 ± 0.32
0.037LeuXaa: 0.037 ± 0.04
Met
1.223MetAla: 1.223 ± 0.25
0.037MetCys: 0.037 ± 0.034
0.889MetAsp: 0.889 ± 0.244
2.038MetGlu: 2.038 ± 0.299
1.037MetPhe: 1.037 ± 0.176
1.074MetGly: 1.074 ± 0.179
0.333MetHis: 0.333 ± 0.111
1.964MetIle: 1.964 ± 0.253
2.594MetLys: 2.594 ± 0.366
1.741MetLeu: 1.741 ± 0.23
0.482MetMet: 0.482 ± 0.13
1.556MetAsn: 1.556 ± 0.227
0.63MetPro: 0.63 ± 0.131
0.963MetGln: 0.963 ± 0.241
0.704MetArg: 0.704 ± 0.156
1.89MetSer: 1.89 ± 0.328
1.667MetThr: 1.667 ± 0.263
1.0MetVal: 1.0 ± 0.208
0.296MetTrp: 0.296 ± 0.099
0.889MetTyr: 0.889 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
3.223AsnAla: 3.223 ± 0.424
0.815AsnCys: 0.815 ± 0.224
4.298AsnAsp: 4.298 ± 0.521
4.224AsnGlu: 4.224 ± 0.51
3.335AsnPhe: 3.335 ± 0.315
4.335AsnGly: 4.335 ± 0.466
1.334AsnHis: 1.334 ± 0.207
6.632AsnIle: 6.632 ± 0.545
7.781AsnLys: 7.781 ± 0.64
5.669AsnLeu: 5.669 ± 0.433
1.853AsnMet: 1.853 ± 0.237
6.632AsnAsn: 6.632 ± 0.701
3.372AsnPro: 3.372 ± 0.341
3.001AsnGln: 3.001 ± 0.322
2.779AsnArg: 2.779 ± 0.316
4.891AsnSer: 4.891 ± 0.481
4.668AsnThr: 4.668 ± 0.459
3.964AsnVal: 3.964 ± 0.398
0.815AsnTrp: 0.815 ± 0.179
3.705AsnTyr: 3.705 ± 0.435
0.0AsnXaa: 0.0 ± 0.0
Pro
1.704ProAla: 1.704 ± 0.307
0.371ProCys: 0.371 ± 0.157
1.63ProAsp: 1.63 ± 0.339
2.631ProGlu: 2.631 ± 0.316
1.927ProPhe: 1.927 ± 0.378
1.89ProGly: 1.89 ± 0.29
0.519ProHis: 0.519 ± 0.15
2.816ProIle: 2.816 ± 0.338
2.297ProLys: 2.297 ± 0.284
2.557ProLeu: 2.557 ± 0.265
0.741ProMet: 0.741 ± 0.122
1.667ProAsn: 1.667 ± 0.297
0.667ProPro: 0.667 ± 0.147
1.037ProGln: 1.037 ± 0.157
0.704ProArg: 0.704 ± 0.17
1.815ProSer: 1.815 ± 0.194
1.853ProThr: 1.853 ± 0.319
2.297ProVal: 2.297 ± 0.3
0.111ProTrp: 0.111 ± 0.073
1.223ProTyr: 1.223 ± 0.202
0.0ProXaa: 0.0 ± 0.0
Gln
1.853GlnAla: 1.853 ± 0.252
0.111GlnCys: 0.111 ± 0.084
2.594GlnAsp: 2.594 ± 0.273
3.631GlnGlu: 3.631 ± 0.461
1.63GlnPhe: 1.63 ± 0.249
1.964GlnGly: 1.964 ± 0.263
0.704GlnHis: 0.704 ± 0.146
2.964GlnIle: 2.964 ± 0.341
3.186GlnLys: 3.186 ± 0.406
3.223GlnLeu: 3.223 ± 0.317
0.815GlnMet: 0.815 ± 0.157
3.298GlnAsn: 3.298 ± 0.333
1.26GlnPro: 1.26 ± 0.197
1.667GlnGln: 1.667 ± 0.235
1.186GlnArg: 1.186 ± 0.183
1.667GlnSer: 1.667 ± 0.262
2.408GlnThr: 2.408 ± 0.329
2.186GlnVal: 2.186 ± 0.264
0.259GlnTrp: 0.259 ± 0.125
1.927GlnTyr: 1.927 ± 0.246
0.0GlnXaa: 0.0 ± 0.0
Arg
1.667ArgAla: 1.667 ± 0.309
0.371ArgCys: 0.371 ± 0.159
2.001ArgAsp: 2.001 ± 0.266
2.594ArgGlu: 2.594 ± 0.327
1.408ArgPhe: 1.408 ± 0.255
1.334ArgGly: 1.334 ± 0.178
0.445ArgHis: 0.445 ± 0.088
2.816ArgIle: 2.816 ± 0.347
3.186ArgLys: 3.186 ± 0.323
2.631ArgLeu: 2.631 ± 0.338
1.074ArgMet: 1.074 ± 0.237
2.408ArgAsn: 2.408 ± 0.232
0.963ArgPro: 0.963 ± 0.215
1.074ArgGln: 1.074 ± 0.173
1.704ArgArg: 1.704 ± 0.379
1.0ArgSer: 1.0 ± 0.178
1.964ArgThr: 1.964 ± 0.242
1.556ArgVal: 1.556 ± 0.27
0.185ArgTrp: 0.185 ± 0.085
0.963ArgTyr: 0.963 ± 0.193
0.0ArgXaa: 0.0 ± 0.0
Ser
2.742SerAla: 2.742 ± 0.305
0.704SerCys: 0.704 ± 0.188
3.335SerAsp: 3.335 ± 0.32
4.001SerGlu: 4.001 ± 0.387
3.112SerPhe: 3.112 ± 0.355
3.483SerGly: 3.483 ± 0.548
1.037SerHis: 1.037 ± 0.2
5.113SerIle: 5.113 ± 0.571
5.669SerLys: 5.669 ± 0.376
6.002SerLeu: 6.002 ± 0.521
1.371SerMet: 1.371 ± 0.221
4.483SerAsn: 4.483 ± 0.593
2.149SerPro: 2.149 ± 0.385
2.223SerGln: 2.223 ± 0.373
1.63SerArg: 1.63 ± 0.248
3.816SerSer: 3.816 ± 0.457
3.372SerThr: 3.372 ± 0.296
3.409SerVal: 3.409 ± 0.369
0.482SerTrp: 0.482 ± 0.136
2.927SerTyr: 2.927 ± 0.555
0.0SerXaa: 0.0 ± 0.0
Thr
2.26ThrAla: 2.26 ± 0.4
0.482ThrCys: 0.482 ± 0.16
3.483ThrAsp: 3.483 ± 0.453
4.076ThrGlu: 4.076 ± 0.392
3.223ThrPhe: 3.223 ± 0.408
4.076ThrGly: 4.076 ± 0.411
1.0ThrHis: 1.0 ± 0.191
5.076ThrIle: 5.076 ± 0.497
4.854ThrLys: 4.854 ± 0.402
5.558ThrLeu: 5.558 ± 0.598
1.408ThrMet: 1.408 ± 0.212
4.817ThrAsn: 4.817 ± 0.45
2.223ThrPro: 2.223 ± 0.25
2.001ThrGln: 2.001 ± 0.297
1.964ThrArg: 1.964 ± 0.253
3.816ThrSer: 3.816 ± 0.324
3.372ThrThr: 3.372 ± 0.518
3.631ThrVal: 3.631 ± 0.367
0.593ThrTrp: 0.593 ± 0.137
2.334ThrTyr: 2.334 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
3.223ValAla: 3.223 ± 0.453
0.445ValCys: 0.445 ± 0.153
3.335ValAsp: 3.335 ± 0.416
4.483ValGlu: 4.483 ± 0.535
2.557ValPhe: 2.557 ± 0.372
2.853ValGly: 2.853 ± 0.351
0.741ValHis: 0.741 ± 0.167
3.927ValIle: 3.927 ± 0.401
4.854ValLys: 4.854 ± 0.371
4.187ValLeu: 4.187 ± 0.415
1.0ValMet: 1.0 ± 0.153
4.483ValAsn: 4.483 ± 0.452
2.112ValPro: 2.112 ± 0.306
2.445ValGln: 2.445 ± 0.263
1.556ValArg: 1.556 ± 0.225
3.335ValSer: 3.335 ± 0.408
3.483ValThr: 3.483 ± 0.449
3.298ValVal: 3.298 ± 0.471
0.371ValTrp: 0.371 ± 0.129
2.482ValTyr: 2.482 ± 0.365
0.0ValXaa: 0.0 ± 0.0
Trp
0.185TrpAla: 0.185 ± 0.093
0.148TrpCys: 0.148 ± 0.077
0.593TrpAsp: 0.593 ± 0.186
0.926TrpGlu: 0.926 ± 0.217
0.371TrpPhe: 0.371 ± 0.171
0.519TrpGly: 0.519 ± 0.141
0.333TrpHis: 0.333 ± 0.173
0.593TrpIle: 0.593 ± 0.194
0.741TrpLys: 0.741 ± 0.161
1.334TrpLeu: 1.334 ± 0.23
0.259TrpMet: 0.259 ± 0.123
0.704TrpAsn: 0.704 ± 0.183
0.0TrpPro: 0.0 ± 0.0
0.519TrpGln: 0.519 ± 0.155
0.445TrpArg: 0.445 ± 0.133
0.445TrpSer: 0.445 ± 0.133
0.482TrpThr: 0.482 ± 0.156
0.408TrpVal: 0.408 ± 0.126
0.037TrpTrp: 0.037 ± 0.039
0.667TrpTyr: 0.667 ± 0.199
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.704TyrAla: 1.704 ± 0.226
0.519TyrCys: 0.519 ± 0.155
3.038TyrAsp: 3.038 ± 0.331
2.594TyrGlu: 2.594 ± 0.325
2.297TyrPhe: 2.297 ± 0.358
2.001TyrGly: 2.001 ± 0.359
0.556TyrHis: 0.556 ± 0.124
3.038TyrIle: 3.038 ± 0.492
3.964TyrLys: 3.964 ± 0.431
3.409TyrLeu: 3.409 ± 0.355
0.778TyrMet: 0.778 ± 0.129
4.298TyrAsn: 4.298 ± 0.536
1.741TyrPro: 1.741 ± 0.377
1.927TyrGln: 1.927 ± 0.294
1.89TyrArg: 1.89 ± 0.213
3.557TyrSer: 3.557 ± 0.383
2.112TyrThr: 2.112 ± 0.224
2.631TyrVal: 2.631 ± 0.327
0.667TyrTrp: 0.667 ± 0.197
2.149TyrTyr: 2.149 ± 0.427
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.037XaaTrp: 0.037 ± 0.04
0.0XaaTyr: 0.0 ± 0.0
0.037XaaXaa: 0.037 ± 0.04
Statistics based on 80 proteins (26991 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski