Tetterwort vein chlorosis virus
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M4MYI6|A0A0M4MYI6_9CLOS Leader protease OS=Tetterwort vein chlorosis virus OX=1712389 PE=4 SV=1
MM1 pKa = 7.32 GLIIVVYY8 pKa = 10.35 KK9 pKa = 8.3 FYY11 pKa = 11.1 CGVNIYY17 pKa = 10.27 YY18 pKa = 10.49 CSNNRR23 pKa = 11.84 DD24 pKa = 3.28 FVGDD28 pKa = 3.72 FEE30 pKa = 5.42 VVTTTEE36 pKa = 4.01 LEE38 pKa = 4.0 KK39 pKa = 10.76 LVEE42 pKa = 4.18 IFEE45 pKa = 4.53 SFPFVRR51 pKa = 11.84 TTWW54 pKa = 3.01
Molecular weight: 6.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.397
IPC2_protein 4.698
IPC_protein 4.406
Toseland 4.266
ProMoST 4.507
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.202
Rodwell 4.253
Grimsley 4.19
Solomon 4.317
Lehninger 4.279
Nozaki 4.469
DTASelect 4.533
Thurlkill 4.279
EMBOSS 4.215
Sillero 4.507
Patrickios 0.693
IPC_peptide 4.329
IPC2_peptide 4.495
IPC2.peptide.svr19 4.477
Protein with the highest isoelectric point:
>tr|A0A0M4LY14|A0A0M4LY14_9CLOS 59.7-kDa protein OS=Tetterwort vein chlorosis virus OX=1712389 PE=4 SV=1
MM1 pKa = 6.83 LTMYY5 pKa = 7.66 RR6 pKa = 11.84 THH8 pKa = 6.96 VIHH11 pKa = 6.98 LFPHH15 pKa = 5.26 GRR17 pKa = 11.84 IWFHH21 pKa = 6.51 EE22 pKa = 4.03 KK23 pKa = 10.45 SIPKK27 pKa = 9.68 QKK29 pKa = 10.24 QNKK32 pKa = 7.47 KK33 pKa = 9.96 QKK35 pKa = 9.28 TKK37 pKa = 9.47 TKK39 pKa = 9.88 QNISRR44 pKa = 11.84 RR45 pKa = 11.84 LYY47 pKa = 10.65 LKK49 pKa = 10.42 LNKK52 pKa = 8.84 FTVVFAHH59 pKa = 6.27 TFRR62 pKa = 11.84 ARR64 pKa = 11.84 CLCAMFRR71 pKa = 11.84 HH72 pKa = 6.12 VSRR75 pKa = 11.84 EE76 pKa = 3.64 RR77 pKa = 3.27
Molecular weight: 9.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.189
IPC2_protein 10.277
IPC_protein 11.418
Toseland 11.623
ProMoST 12.032
Dawson 11.637
Bjellqvist 11.55
Wikipedia 12.032
Rodwell 11.74
Grimsley 11.681
Solomon 12.032
Lehninger 11.959
Nozaki 11.608
DTASelect 11.55
Thurlkill 11.608
EMBOSS 12.091
Sillero 11.608
Patrickios 11.462
IPC_peptide 12.047
IPC2_peptide 10.979
IPC2.peptide.svr19 8.976
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
4987
44
1992
383.6
44.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.91 ± 0.459
2.206 ± 0.285
6.517 ± 0.351
5.153 ± 0.272
5.875 ± 0.53
4.833 ± 0.246
2.065 ± 0.245
6.818 ± 0.372
7.7 ± 0.292
9.505 ± 0.642
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.847 ± 0.256
6.357 ± 0.683
3.128 ± 0.271
2.727 ± 0.247
5.354 ± 0.579
7.419 ± 0.396
5.474 ± 0.442
6.918 ± 0.384
0.862 ± 0.153
4.331 ± 0.421
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here