Mycobacterium phage Ejimix

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Omegavirus; unclassified Omegavirus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385D355|A0A385D355_9CAUD Uncharacterized protein OS=Mycobacterium phage Ejimix OX=2301550 GN=106 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.09IIVEE6 pKa = 3.96IDD8 pKa = 3.17YY9 pKa = 11.05DD10 pKa = 4.32PNLSYY15 pKa = 11.33YY16 pKa = 9.52PDD18 pKa = 3.61EE19 pKa = 4.32VTTKK23 pKa = 10.94LEE25 pKa = 4.02ALQFEE30 pKa = 4.79VAALEE35 pKa = 4.48EE36 pKa = 4.52GTTDD40 pKa = 2.33IGMFIPHH47 pKa = 6.83MGDD50 pKa = 2.67IYY52 pKa = 11.51VVDD55 pKa = 4.4DD56 pKa = 4.34DD57 pKa = 4.88GVRR60 pKa = 11.84VDD62 pKa = 3.76

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385D3A7|A0A385D3A7_9CAUD Uncharacterized protein OS=Mycobacterium phage Ejimix OX=2301550 GN=157 PE=4 SV=1
MM1 pKa = 6.49THH3 pKa = 7.07RR4 pKa = 11.84GLEE7 pKa = 3.97RR8 pKa = 11.84AEE10 pKa = 3.92EE11 pKa = 3.95RR12 pKa = 11.84RR13 pKa = 11.84VQIAKK18 pKa = 10.48LIRR21 pKa = 11.84QGLTNKK27 pKa = 9.43EE28 pKa = 3.79VAQIVGVTDD37 pKa = 3.29RR38 pKa = 11.84TVNRR42 pKa = 11.84ARR44 pKa = 11.84EE45 pKa = 4.02RR46 pKa = 11.84KK47 pKa = 9.19GLKK50 pKa = 9.77KK51 pKa = 9.73PQPPRR56 pKa = 11.84ISEE59 pKa = 4.07EE60 pKa = 4.17TLSAGYY66 pKa = 10.86QMLLDD71 pKa = 4.0GCSYY75 pKa = 11.69AEE77 pKa = 3.99VARR80 pKa = 11.84TLGHH84 pKa = 6.73SRR86 pKa = 11.84GAWRR90 pKa = 11.84KK91 pKa = 9.21KK92 pKa = 10.09FPGFSWSHH100 pKa = 4.68VEE102 pKa = 3.73CGRR105 pKa = 11.84FRR107 pKa = 11.84KK108 pKa = 9.42LQEE111 pKa = 4.09RR112 pKa = 11.84YY113 pKa = 9.93EE114 pKa = 4.26GLLL117 pKa = 3.55

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

234

0

234

34728

30

1640

148.4

16.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.764 ± 0.238

1.457 ± 0.135

6.761 ± 0.149

6.934 ± 0.237

3.124 ± 0.088

8.425 ± 0.347

2.292 ± 0.127

4.826 ± 0.145

3.951 ± 0.195

8.06 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.084

3.355 ± 0.12

5.327 ± 0.173

3.142 ± 0.159

6.675 ± 0.225

5.37 ± 0.168

5.609 ± 0.226

6.968 ± 0.229

2.378 ± 0.12

3.095 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski