Aspergillus avenaceus
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11290 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6TK27|A0A5N6TK27_9EURO EKC/KEOPS complex subunit CGI121 OS=Aspergillus avenaceus OX=36643 GN=BDV25DRAFT_162447 PE=3 SV=1
MM1 pKa = 7.13 HH2 pKa = 7.6 TIKK5 pKa = 10.91 LLAFSGLMLMSSAIGAEE22 pKa = 4.34 LDD24 pKa = 3.76 RR25 pKa = 11.84 DD26 pKa = 4.05 DD27 pKa = 4.88 VPNRR31 pKa = 11.84 CWDD34 pKa = 3.12 VCGPVVGIAHH44 pKa = 7.05 KK45 pKa = 10.45 CDD47 pKa = 2.96 GMHH50 pKa = 7.39 DD51 pKa = 3.72 SDD53 pKa = 4.11 RR54 pKa = 11.84 AEE56 pKa = 4.27 MNCICDD62 pKa = 3.54 WKK64 pKa = 10.35 QAPSLIPLCEE74 pKa = 3.75 ACIAQYY80 pKa = 10.43 RR81 pKa = 11.84 SEE83 pKa = 4.09 RR84 pKa = 11.84 NQRR87 pKa = 11.84 NRR89 pKa = 11.84 DD90 pKa = 3.71 DD91 pKa = 6.33 DD92 pKa = 6.3 DD93 pKa = 7.57 DD94 pKa = 7.29 DD95 pKa = 7.65 DD96 pKa = 7.58 DD97 pKa = 7.43 DD98 pKa = 6.68 DD99 pKa = 5.92 DD100 pKa = 6.22 HH101 pKa = 7.73 DD102 pKa = 4.12 PHH104 pKa = 8.73 DD105 pKa = 4.17 NDD107 pKa = 5.09 AYY109 pKa = 10.86 DD110 pKa = 3.77 ILTSCSLSTTSYY122 pKa = 10.79 NPTAASSAVSSASATGNASATDD144 pKa = 3.63 ATSTSTATGSVSNSPVSDD162 pKa = 3.56 SASTSNTNSANNAQTTDD179 pKa = 3.27 NAASSFSSPASASLAAVVGLGFLAWLL205 pKa = 4.18
Molecular weight: 21.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.91
IPC_protein 3.935
Toseland 3.694
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.935
Rodwell 3.757
Grimsley 3.605
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.406
Thurlkill 3.77
EMBOSS 3.948
Sillero 4.062
Patrickios 1.952
IPC_peptide 3.935
IPC2_peptide 4.037
IPC2.peptide.svr19 3.946
Protein with the highest isoelectric point:
>tr|A0A5N6U7K7|A0A5N6U7K7_9EURO Chaperonin 10-like protein OS=Aspergillus avenaceus OX=36643 GN=BDV25DRAFT_147490 PE=4 SV=1
MM1 pKa = 6.63 TTRR4 pKa = 11.84 TPRR7 pKa = 11.84 PPTGMPTPIPRR18 pKa = 11.84 PPRR21 pKa = 11.84 PPATEE26 pKa = 3.83 AHH28 pKa = 6.93 PPTPPHH34 pKa = 6.94 LPIQTLRR41 pKa = 11.84 TRR43 pKa = 11.84 LRR45 pKa = 11.84 LPTPAPRR52 pKa = 11.84 PTQAPPTAPPPTPTPRR68 pKa = 11.84 PTVVRR73 pKa = 11.84 LPTALRR79 pKa = 11.84 LTAVTLPAPITAVTLMHH96 pKa = 6.89 PRR98 pKa = 11.84 PPVNVQQ104 pKa = 2.65
Molecular weight: 11.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.945
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11290
0
11290
5386922
49
6023
477.1
52.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.153 ± 0.017
1.342 ± 0.011
5.663 ± 0.014
6.036 ± 0.023
3.838 ± 0.014
6.766 ± 0.024
2.475 ± 0.01
5.057 ± 0.017
4.577 ± 0.021
9.138 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.199 ± 0.008
3.716 ± 0.011
5.952 ± 0.026
4.018 ± 0.016
6.093 ± 0.021
8.29 ± 0.025
5.966 ± 0.014
6.281 ± 0.019
1.502 ± 0.008
2.938 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here