Amino acid dipepetide frequency for Rhizobium phage B1VFA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.494AlaAla: 15.494 ± 1.154
0.817AlaCys: 0.817 ± 0.142
7.379AlaAsp: 7.379 ± 0.519
7.216AlaGlu: 7.216 ± 0.564
3.676AlaPhe: 3.676 ± 0.289
7.869AlaGly: 7.869 ± 0.447
1.525AlaHis: 1.525 ± 0.248
5.664AlaIle: 5.664 ± 0.322
6.263AlaLys: 6.263 ± 0.482
8.958AlaLeu: 8.958 ± 0.535
3.594AlaMet: 3.594 ± 0.301
3.839AlaAsn: 3.839 ± 0.416
3.921AlaPro: 3.921 ± 0.357
3.975AlaGln: 3.975 ± 0.328
5.283AlaArg: 5.283 ± 0.401
5.473AlaSer: 5.473 ± 0.357
5.909AlaThr: 5.909 ± 0.635
6.481AlaVal: 6.481 ± 0.422
1.089AlaTrp: 1.089 ± 0.188
3.186AlaTyr: 3.186 ± 0.282
0.0AlaXaa: 0.0 ± 0.0
Cys
0.654CysAla: 0.654 ± 0.143
0.136CysCys: 0.136 ± 0.062
0.517CysAsp: 0.517 ± 0.114
0.436CysGlu: 0.436 ± 0.11
0.245CysPhe: 0.245 ± 0.078
0.681CysGly: 0.681 ± 0.166
0.082CysHis: 0.082 ± 0.04
0.3CysIle: 0.3 ± 0.075
0.463CysLys: 0.463 ± 0.117
0.599CysLeu: 0.599 ± 0.135
0.218CysMet: 0.218 ± 0.096
0.245CysAsn: 0.245 ± 0.077
0.463CysPro: 0.463 ± 0.126
0.436CysGln: 0.436 ± 0.115
0.599CysArg: 0.599 ± 0.126
0.327CysSer: 0.327 ± 0.095
0.408CysThr: 0.408 ± 0.111
0.654CysVal: 0.654 ± 0.159
0.109CysTrp: 0.109 ± 0.047
0.381CysTyr: 0.381 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
6.78AspAla: 6.78 ± 0.427
0.545AspCys: 0.545 ± 0.115
4.629AspAsp: 4.629 ± 0.613
4.575AspGlu: 4.575 ± 0.415
2.696AspPhe: 2.696 ± 0.267
5.283AspGly: 5.283 ± 0.379
1.498AspHis: 1.498 ± 0.179
3.567AspIle: 3.567 ± 0.299
3.268AspLys: 3.268 ± 0.317
5.963AspLeu: 5.963 ± 0.4
1.443AspMet: 1.443 ± 0.208
2.614AspAsn: 2.614 ± 0.24
3.73AspPro: 3.73 ± 0.364
1.852AspGln: 1.852 ± 0.207
3.839AspArg: 3.839 ± 0.347
2.886AspSer: 2.886 ± 0.253
3.131AspThr: 3.131 ± 0.346
2.968AspVal: 2.968 ± 0.259
1.144AspTrp: 1.144 ± 0.156
1.906AspTyr: 1.906 ± 0.238
0.0AspXaa: 0.0 ± 0.0
Glu
8.033GluAla: 8.033 ± 0.605
0.49GluCys: 0.49 ± 0.145
3.812GluAsp: 3.812 ± 0.507
5.609GluGlu: 5.609 ± 0.565
2.423GluPhe: 2.423 ± 0.253
4.112GluGly: 4.112 ± 0.285
1.225GluHis: 1.225 ± 0.194
4.139GluIle: 4.139 ± 0.366
4.547GluLys: 4.547 ± 0.418
6.835GluLeu: 6.835 ± 0.426
2.069GluMet: 2.069 ± 0.26
2.314GluAsn: 2.314 ± 0.221
2.015GluPro: 2.015 ± 0.264
2.342GluGln: 2.342 ± 0.253
4.602GluArg: 4.602 ± 0.469
2.505GluSer: 2.505 ± 0.283
3.948GluThr: 3.948 ± 0.302
4.629GluVal: 4.629 ± 0.386
0.926GluTrp: 0.926 ± 0.163
1.879GluTyr: 1.879 ± 0.255
0.0GluXaa: 0.0 ± 0.0
Phe
3.594PheAla: 3.594 ± 0.272
0.436PheCys: 0.436 ± 0.104
2.668PheAsp: 2.668 ± 0.267
2.641PheGlu: 2.641 ± 0.309
1.307PhePhe: 1.307 ± 0.164
3.458PheGly: 3.458 ± 0.348
0.762PheHis: 0.762 ± 0.157
1.579PheIle: 1.579 ± 0.214
2.478PheLys: 2.478 ± 0.255
3.077PheLeu: 3.077 ± 0.3
1.361PheMet: 1.361 ± 0.199
1.906PheAsn: 1.906 ± 0.217
1.307PhePro: 1.307 ± 0.19
1.089PheGln: 1.089 ± 0.171
2.069PheArg: 2.069 ± 0.259
2.233PheSer: 2.233 ± 0.246
2.178PheThr: 2.178 ± 0.224
2.532PheVal: 2.532 ± 0.238
0.408PheTrp: 0.408 ± 0.101
1.361PheTyr: 1.361 ± 0.22
0.0PheXaa: 0.0 ± 0.0
Gly
6.644GlyAla: 6.644 ± 0.473
0.817GlyCys: 0.817 ± 0.138
4.411GlyAsp: 4.411 ± 0.409
5.037GlyGlu: 5.037 ± 0.383
3.186GlyPhe: 3.186 ± 0.294
5.718GlyGly: 5.718 ± 0.522
1.389GlyHis: 1.389 ± 0.178
4.03GlyIle: 4.03 ± 0.299
4.956GlyLys: 4.956 ± 0.395
5.854GlyLeu: 5.854 ± 0.471
1.988GlyMet: 1.988 ± 0.243
3.05GlyAsn: 3.05 ± 0.314
2.342GlyPro: 2.342 ± 0.275
2.805GlyGln: 2.805 ± 0.281
3.594GlyArg: 3.594 ± 0.351
4.656GlySer: 4.656 ± 0.417
4.329GlyThr: 4.329 ± 0.457
5.174GlyVal: 5.174 ± 0.375
1.334GlyTrp: 1.334 ± 0.181
2.505GlyTyr: 2.505 ± 0.285
0.0GlyXaa: 0.0 ± 0.0
His
1.797HisAla: 1.797 ± 0.2
0.082HisCys: 0.082 ± 0.049
1.144HisAsp: 1.144 ± 0.158
1.089HisGlu: 1.089 ± 0.166
0.899HisPhe: 0.899 ± 0.175
1.661HisGly: 1.661 ± 0.226
0.436HisHis: 0.436 ± 0.119
1.116HisIle: 1.116 ± 0.18
1.062HisLys: 1.062 ± 0.155
1.661HisLeu: 1.661 ± 0.241
0.463HisMet: 0.463 ± 0.13
0.654HisAsn: 0.654 ± 0.128
1.089HisPro: 1.089 ± 0.167
0.517HisGln: 0.517 ± 0.121
1.28HisArg: 1.28 ± 0.172
0.953HisSer: 0.953 ± 0.167
1.035HisThr: 1.035 ± 0.16
1.116HisVal: 1.116 ± 0.175
0.327HisTrp: 0.327 ± 0.082
0.517HisTyr: 0.517 ± 0.111
0.0HisXaa: 0.0 ± 0.0
Ile
6.78IleAla: 6.78 ± 0.392
0.463IleCys: 0.463 ± 0.11
3.921IleAsp: 3.921 ± 0.278
4.602IleGlu: 4.602 ± 0.388
1.416IlePhe: 1.416 ± 0.217
3.867IleGly: 3.867 ± 0.309
1.225IleHis: 1.225 ± 0.204
3.186IleIle: 3.186 ± 0.256
3.022IleLys: 3.022 ± 0.336
4.302IleLeu: 4.302 ± 0.341
1.416IleMet: 1.416 ± 0.201
2.233IleAsn: 2.233 ± 0.228
2.342IlePro: 2.342 ± 0.26
1.77IleGln: 1.77 ± 0.216
3.622IleArg: 3.622 ± 0.285
3.295IleSer: 3.295 ± 0.275
3.485IleThr: 3.485 ± 0.32
4.057IleVal: 4.057 ± 0.413
0.79IleTrp: 0.79 ± 0.148
1.607IleTyr: 1.607 ± 0.198
0.0IleXaa: 0.0 ± 0.0
Lys
6.508LysAla: 6.508 ± 0.429
0.191LysCys: 0.191 ± 0.074
3.812LysAsp: 3.812 ± 0.388
4.166LysGlu: 4.166 ± 0.396
2.233LysPhe: 2.233 ± 0.29
3.921LysGly: 3.921 ± 0.351
1.443LysHis: 1.443 ± 0.236
3.676LysIle: 3.676 ± 0.327
4.52LysLys: 4.52 ± 0.459
4.683LysLeu: 4.683 ± 0.39
1.661LysMet: 1.661 ± 0.223
1.879LysAsn: 1.879 ± 0.209
2.696LysPro: 2.696 ± 0.334
2.042LysGln: 2.042 ± 0.24
3.73LysArg: 3.73 ± 0.436
2.614LysSer: 2.614 ± 0.255
3.867LysThr: 3.867 ± 0.306
4.329LysVal: 4.329 ± 0.343
0.654LysTrp: 0.654 ± 0.147
1.198LysTyr: 1.198 ± 0.174
0.0LysXaa: 0.0 ± 0.0
Leu
8.795LeuAla: 8.795 ± 0.503
0.49LeuCys: 0.49 ± 0.136
5.446LeuAsp: 5.446 ± 0.29
5.473LeuGlu: 5.473 ± 0.327
2.532LeuPhe: 2.532 ± 0.263
5.092LeuGly: 5.092 ± 0.404
1.852LeuHis: 1.852 ± 0.229
4.302LeuIle: 4.302 ± 0.361
5.364LeuLys: 5.364 ± 0.399
5.582LeuLeu: 5.582 ± 0.403
1.688LeuMet: 1.688 ± 0.214
3.404LeuAsn: 3.404 ± 0.311
4.411LeuPro: 4.411 ± 0.306
2.696LeuGln: 2.696 ± 0.406
4.765LeuArg: 4.765 ± 0.46
4.52LeuSer: 4.52 ± 0.33
5.909LeuThr: 5.909 ± 0.423
5.664LeuVal: 5.664 ± 0.376
0.926LeuTrp: 0.926 ± 0.163
2.178LeuTyr: 2.178 ± 0.224
0.0LeuXaa: 0.0 ± 0.0
Met
2.587MetAla: 2.587 ± 0.294
0.136MetCys: 0.136 ± 0.057
1.089MetAsp: 1.089 ± 0.159
1.47MetGlu: 1.47 ± 0.227
0.926MetPhe: 0.926 ± 0.188
1.906MetGly: 1.906 ± 0.221
0.463MetHis: 0.463 ± 0.109
1.416MetIle: 1.416 ± 0.169
2.042MetLys: 2.042 ± 0.223
1.443MetLeu: 1.443 ± 0.187
0.735MetMet: 0.735 ± 0.154
1.171MetAsn: 1.171 ± 0.19
1.715MetPro: 1.715 ± 0.208
1.062MetGln: 1.062 ± 0.171
2.042MetArg: 2.042 ± 0.252
2.478MetSer: 2.478 ± 0.279
2.151MetThr: 2.151 ± 0.242
1.47MetVal: 1.47 ± 0.205
0.136MetTrp: 0.136 ± 0.057
0.354MetTyr: 0.354 ± 0.091
0.0MetXaa: 0.0 ± 0.0
Asn
3.839AsnAla: 3.839 ± 0.322
0.408AsnCys: 0.408 ± 0.115
2.478AsnAsp: 2.478 ± 0.237
2.505AsnGlu: 2.505 ± 0.203
1.661AsnPhe: 1.661 ± 0.196
3.376AsnGly: 3.376 ± 0.405
0.735AsnHis: 0.735 ± 0.129
2.26AsnIle: 2.26 ± 0.241
1.797AsnLys: 1.797 ± 0.231
2.805AsnLeu: 2.805 ± 0.266
1.089AsnMet: 1.089 ± 0.196
1.743AsnAsn: 1.743 ± 0.256
1.852AsnPro: 1.852 ± 0.186
1.198AsnGln: 1.198 ± 0.227
2.151AsnArg: 2.151 ± 0.241
1.579AsnSer: 1.579 ± 0.22
2.505AsnThr: 2.505 ± 0.391
2.396AsnVal: 2.396 ± 0.278
0.599AsnTrp: 0.599 ± 0.115
1.198AsnTyr: 1.198 ± 0.161
0.0AsnXaa: 0.0 ± 0.0
Pro
5.174ProAla: 5.174 ± 0.436
0.245ProCys: 0.245 ± 0.081
3.376ProAsp: 3.376 ± 0.251
3.594ProGlu: 3.594 ± 0.347
1.797ProPhe: 1.797 ± 0.237
3.131ProGly: 3.131 ± 0.297
0.817ProHis: 0.817 ± 0.134
2.75ProIle: 2.75 ± 0.283
2.042ProLys: 2.042 ± 0.243
3.131ProLeu: 3.131 ± 0.304
1.035ProMet: 1.035 ± 0.18
1.171ProAsn: 1.171 ± 0.161
1.198ProPro: 1.198 ± 0.224
1.334ProGln: 1.334 ± 0.179
1.879ProArg: 1.879 ± 0.241
2.587ProSer: 2.587 ± 0.301
2.423ProThr: 2.423 ± 0.277
3.022ProVal: 3.022 ± 0.323
0.517ProTrp: 0.517 ± 0.115
0.98ProTyr: 0.98 ± 0.137
0.0ProXaa: 0.0 ± 0.0
Gln
3.921GlnAla: 3.921 ± 0.376
0.163GlnCys: 0.163 ± 0.06
1.661GlnAsp: 1.661 ± 0.229
2.696GlnGlu: 2.696 ± 0.296
1.661GlnPhe: 1.661 ± 0.209
2.369GlnGly: 2.369 ± 0.35
0.436GlnHis: 0.436 ± 0.112
2.505GlnIle: 2.505 ± 0.2
2.369GlnLys: 2.369 ± 0.273
3.186GlnLeu: 3.186 ± 0.41
1.035GlnMet: 1.035 ± 0.162
1.389GlnAsn: 1.389 ± 0.209
1.552GlnPro: 1.552 ± 0.208
1.416GlnGln: 1.416 ± 0.291
1.906GlnArg: 1.906 ± 0.272
1.525GlnSer: 1.525 ± 0.244
1.797GlnThr: 1.797 ± 0.283
2.696GlnVal: 2.696 ± 0.285
0.49GlnTrp: 0.49 ± 0.159
0.654GlnTyr: 0.654 ± 0.119
0.0GlnXaa: 0.0 ± 0.0
Arg
5.609ArgAla: 5.609 ± 0.34
0.463ArgCys: 0.463 ± 0.125
3.458ArgAsp: 3.458 ± 0.316
3.948ArgGlu: 3.948 ± 0.45
2.641ArgPhe: 2.641 ± 0.239
4.112ArgGly: 4.112 ± 0.33
1.198ArgHis: 1.198 ± 0.143
3.676ArgIle: 3.676 ± 0.306
3.24ArgLys: 3.24 ± 0.334
5.31ArgLeu: 5.31 ± 0.401
1.416ArgMet: 1.416 ± 0.221
2.124ArgAsn: 2.124 ± 0.226
2.015ArgPro: 2.015 ± 0.255
2.369ArgGln: 2.369 ± 0.25
3.785ArgArg: 3.785 ± 0.423
2.75ArgSer: 2.75 ± 0.262
3.376ArgThr: 3.376 ± 0.311
4.112ArgVal: 4.112 ± 0.291
0.871ArgTrp: 0.871 ± 0.185
1.743ArgTyr: 1.743 ± 0.218
0.0ArgXaa: 0.0 ± 0.0
Ser
5.255SerAla: 5.255 ± 0.504
0.49SerCys: 0.49 ± 0.113
3.24SerAsp: 3.24 ± 0.312
2.75SerGlu: 2.75 ± 0.279
2.314SerPhe: 2.314 ± 0.238
4.629SerGly: 4.629 ± 0.417
0.953SerHis: 0.953 ± 0.188
3.104SerIle: 3.104 ± 0.271
2.941SerLys: 2.941 ± 0.267
4.057SerLeu: 4.057 ± 0.409
1.607SerMet: 1.607 ± 0.229
2.287SerAsn: 2.287 ± 0.362
2.396SerPro: 2.396 ± 0.264
2.287SerGln: 2.287 ± 0.257
2.641SerArg: 2.641 ± 0.28
3.295SerSer: 3.295 ± 0.324
2.614SerThr: 2.614 ± 0.33
3.567SerVal: 3.567 ± 0.297
0.762SerTrp: 0.762 ± 0.139
1.77SerTyr: 1.77 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
5.963ThrAla: 5.963 ± 0.575
0.381ThrCys: 0.381 ± 0.101
3.131ThrAsp: 3.131 ± 0.33
3.458ThrGlu: 3.458 ± 0.353
2.178ThrPhe: 2.178 ± 0.21
5.5ThrGly: 5.5 ± 0.485
0.79ThrHis: 0.79 ± 0.123
4.084ThrIle: 4.084 ± 0.391
3.159ThrLys: 3.159 ± 0.269
5.419ThrLeu: 5.419 ± 0.413
1.144ThrMet: 1.144 ± 0.162
2.423ThrAsn: 2.423 ± 0.301
3.131ThrPro: 3.131 ± 0.289
1.852ThrGln: 1.852 ± 0.25
3.349ThrArg: 3.349 ± 0.327
3.186ThrSer: 3.186 ± 0.534
4.384ThrThr: 4.384 ± 0.511
4.139ThrVal: 4.139 ± 0.461
0.953ThrTrp: 0.953 ± 0.164
1.688ThrTyr: 1.688 ± 0.231
0.0ThrXaa: 0.0 ± 0.0
Val
6.943ValAla: 6.943 ± 0.397
0.654ValCys: 0.654 ± 0.143
4.493ValAsp: 4.493 ± 0.447
4.711ValGlu: 4.711 ± 0.402
2.777ValPhe: 2.777 ± 0.27
4.329ValGly: 4.329 ± 0.348
1.007ValHis: 1.007 ± 0.162
3.785ValIle: 3.785 ± 0.281
4.112ValLys: 4.112 ± 0.332
4.792ValLeu: 4.792 ± 0.334
1.879ValMet: 1.879 ± 0.178
2.26ValAsn: 2.26 ± 0.269
2.696ValPro: 2.696 ± 0.249
2.641ValGln: 2.641 ± 0.268
3.867ValArg: 3.867 ± 0.354
3.948ValSer: 3.948 ± 0.325
4.384ValThr: 4.384 ± 0.406
5.119ValVal: 5.119 ± 0.385
0.517ValTrp: 0.517 ± 0.138
1.852ValTyr: 1.852 ± 0.213
0.0ValXaa: 0.0 ± 0.0
Trp
0.844TrpAla: 0.844 ± 0.145
0.191TrpCys: 0.191 ± 0.068
1.144TrpAsp: 1.144 ± 0.168
0.926TrpGlu: 0.926 ± 0.155
0.572TrpPhe: 0.572 ± 0.11
0.681TrpGly: 0.681 ± 0.133
0.49TrpHis: 0.49 ± 0.12
0.79TrpIle: 0.79 ± 0.148
0.98TrpLys: 0.98 ± 0.152
0.953TrpLeu: 0.953 ± 0.149
0.327TrpMet: 0.327 ± 0.093
0.599TrpAsn: 0.599 ± 0.143
0.245TrpPro: 0.245 ± 0.084
0.354TrpGln: 0.354 ± 0.097
1.062TrpArg: 1.062 ± 0.17
0.599TrpSer: 0.599 ± 0.143
0.953TrpThr: 0.953 ± 0.19
0.926TrpVal: 0.926 ± 0.145
0.163TrpTrp: 0.163 ± 0.066
0.354TrpTyr: 0.354 ± 0.079
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.396TyrAla: 2.396 ± 0.259
0.354TyrCys: 0.354 ± 0.091
2.505TyrAsp: 2.505 ± 0.284
1.552TyrGlu: 1.552 ± 0.242
1.334TyrPhe: 1.334 ± 0.206
2.124TyrGly: 2.124 ± 0.244
0.572TyrHis: 0.572 ± 0.121
1.47TyrIle: 1.47 ± 0.223
1.198TyrLys: 1.198 ± 0.202
2.478TyrLeu: 2.478 ± 0.257
0.599TyrMet: 0.599 ± 0.146
0.871TyrAsn: 0.871 ± 0.148
1.089TyrPro: 1.089 ± 0.16
1.389TyrGln: 1.389 ± 0.188
2.151TyrArg: 2.151 ± 0.234
1.552TyrSer: 1.552 ± 0.213
1.525TyrThr: 1.525 ± 0.282
1.715TyrVal: 1.715 ± 0.229
0.436TyrTrp: 0.436 ± 0.123
0.871TyrTyr: 0.871 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 160 proteins (36726 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski