Sulcia muelleri (strain GWSS)
Average proteome isoelectric point is 8.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 227 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8Z5Z9|A8Z5Z9_SULMW Threonine synthase OS=Sulcia muelleri (strain GWSS) OX=444179 GN=thrC PE=3 SV=1
MM1 pKa = 7.31 SLKK4 pKa = 9.15 ITEE7 pKa = 4.06 KK8 pKa = 10.7 CINCGACEE16 pKa = 4.03 PEE18 pKa = 4.51 CPNNAIYY25 pKa = 10.43 EE26 pKa = 4.45 GEE28 pKa = 4.26 CKK30 pKa = 10.2 KK31 pKa = 10.68 NYY33 pKa = 10.25 YY34 pKa = 10.0 YY35 pKa = 10.75 FIVPEE40 pKa = 4.39 KK41 pKa = 10.09 CTEE44 pKa = 3.88 CLGFYY49 pKa = 10.46 DD50 pKa = 4.83 EE51 pKa = 4.73 PQCASVCPVDD61 pKa = 5.06 CCILDD66 pKa = 3.66 EE67 pKa = 4.59 EE68 pKa = 4.8 NIEE71 pKa = 4.47 SEE73 pKa = 3.86 EE74 pKa = 4.45 DD75 pKa = 3.19 LFKK78 pKa = 10.96 KK79 pKa = 10.23 INYY82 pKa = 7.89 VRR84 pKa = 11.84 NLL86 pKa = 3.3
Molecular weight: 9.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.296
IPC2_protein 4.393
IPC_protein 4.24
Toseland 4.101
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.012
Rodwell 4.075
Grimsley 4.012
Solomon 4.151
Lehninger 4.113
Nozaki 4.279
DTASelect 4.355
Thurlkill 4.101
EMBOSS 4.024
Sillero 4.342
Patrickios 0.057
IPC_peptide 4.164
IPC2_peptide 4.329
IPC2.peptide.svr19 4.278
Protein with the highest isoelectric point:
>tr|A8Z667|A8Z667_SULMW 30S ribosomal protein S10 OS=Sulcia muelleri (strain GWSS) OX=444179 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.51 PTIQQLVRR9 pKa = 11.84 KK10 pKa = 8.92 GRR12 pKa = 11.84 KK13 pKa = 8.86 NIKK16 pKa = 9.85 KK17 pKa = 9.59 KK18 pKa = 10.16 SKK20 pKa = 10.83 SMALNCCPQRR30 pKa = 11.84 KK31 pKa = 8.68 GVCTRR36 pKa = 11.84 VYY38 pKa = 7.88 TTTPKK43 pKa = 10.54 KK44 pKa = 10.11 PNSAMRR50 pKa = 11.84 KK51 pKa = 5.78 VARR54 pKa = 11.84 VRR56 pKa = 11.84 LTNGKK61 pKa = 8.69 EE62 pKa = 3.69 VNAYY66 pKa = 9.63 IPGEE70 pKa = 3.91 GHH72 pKa = 6.47 NLQEE76 pKa = 4.15 HH77 pKa = 6.65 SIVLIRR83 pKa = 11.84 GGRR86 pKa = 11.84 VKK88 pKa = 10.7 DD89 pKa = 3.73 LPGVRR94 pKa = 11.84 YY95 pKa = 9.54 HH96 pKa = 7.67 IIRR99 pKa = 11.84 GALDD103 pKa = 3.28 TAGVKK108 pKa = 9.56 DD109 pKa = 3.87 RR110 pKa = 11.84 KK111 pKa = 9.65 KK112 pKa = 10.8 ARR114 pKa = 11.84 SKK116 pKa = 11.26 YY117 pKa = 9.5 GAKK120 pKa = 9.98 CSKK123 pKa = 10.2 KK124 pKa = 9.68 INKK127 pKa = 8.98 INKK130 pKa = 8.7 KK131 pKa = 10.02
Molecular weight: 14.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.284
IPC2_protein 10.028
IPC_protein 10.701
Toseland 11.169
ProMoST 10.847
Dawson 11.213
Bjellqvist 10.906
Wikipedia 11.403
Rodwell 11.637
Grimsley 11.242
Solomon 11.359
Lehninger 11.33
Nozaki 11.14
DTASelect 10.891
Thurlkill 11.14
EMBOSS 11.564
Sillero 11.155
Patrickios 11.345
IPC_peptide 11.374
IPC2_peptide 9.853
IPC2.peptide.svr19 8.387
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
227
0
227
75114
38
1464
330.9
38.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.481 ± 0.137
1.158 ± 0.048
4.023 ± 0.096
5.287 ± 0.142
5.412 ± 0.171
5.576 ± 0.149
1.401 ± 0.048
13.589 ± 0.18
12.641 ± 0.217
8.94 ± 0.108
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.763 ± 0.06
8.434 ± 0.164
2.761 ± 0.063
1.886 ± 0.053
3.033 ± 0.095
6.703 ± 0.13
4.021 ± 0.078
4.049 ± 0.112
0.644 ± 0.063
5.2 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here