Sulcia muelleri (strain GWSS)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriales incertae sedis; Candidatus Sulcia; Candidatus Sulcia muelleri

Average proteome isoelectric point is 8.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A8Z5Z9|A8Z5Z9_SULMW Threonine synthase OS=Sulcia muelleri (strain GWSS) OX=444179 GN=thrC PE=3 SV=1
MM1 pKa = 7.31SLKK4 pKa = 9.15ITEE7 pKa = 4.06KK8 pKa = 10.7CINCGACEE16 pKa = 4.03PEE18 pKa = 4.51CPNNAIYY25 pKa = 10.43EE26 pKa = 4.45GEE28 pKa = 4.26CKK30 pKa = 10.2KK31 pKa = 10.68NYY33 pKa = 10.25YY34 pKa = 10.0YY35 pKa = 10.75FIVPEE40 pKa = 4.39KK41 pKa = 10.09CTEE44 pKa = 3.88CLGFYY49 pKa = 10.46DD50 pKa = 4.83EE51 pKa = 4.73PQCASVCPVDD61 pKa = 5.06CCILDD66 pKa = 3.66EE67 pKa = 4.59EE68 pKa = 4.8NIEE71 pKa = 4.47SEE73 pKa = 3.86EE74 pKa = 4.45DD75 pKa = 3.19LFKK78 pKa = 10.96KK79 pKa = 10.23INYY82 pKa = 7.89VRR84 pKa = 11.84NLL86 pKa = 3.3

Molecular weight:
9.91 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A8Z667|A8Z667_SULMW 30S ribosomal protein S10 OS=Sulcia muelleri (strain GWSS) OX=444179 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.51PTIQQLVRR9 pKa = 11.84KK10 pKa = 8.92GRR12 pKa = 11.84KK13 pKa = 8.86NIKK16 pKa = 9.85KK17 pKa = 9.59KK18 pKa = 10.16SKK20 pKa = 10.83SMALNCCPQRR30 pKa = 11.84KK31 pKa = 8.68GVCTRR36 pKa = 11.84VYY38 pKa = 7.88TTTPKK43 pKa = 10.54KK44 pKa = 10.11PNSAMRR50 pKa = 11.84KK51 pKa = 5.78VARR54 pKa = 11.84VRR56 pKa = 11.84LTNGKK61 pKa = 8.69EE62 pKa = 3.69VNAYY66 pKa = 9.63IPGEE70 pKa = 3.91GHH72 pKa = 6.47NLQEE76 pKa = 4.15HH77 pKa = 6.65SIVLIRR83 pKa = 11.84GGRR86 pKa = 11.84VKK88 pKa = 10.7DD89 pKa = 3.73LPGVRR94 pKa = 11.84YY95 pKa = 9.54HH96 pKa = 7.67IIRR99 pKa = 11.84GALDD103 pKa = 3.28TAGVKK108 pKa = 9.56DD109 pKa = 3.87RR110 pKa = 11.84KK111 pKa = 9.65KK112 pKa = 10.8ARR114 pKa = 11.84SKK116 pKa = 11.26YY117 pKa = 9.5GAKK120 pKa = 9.98CSKK123 pKa = 10.2KK124 pKa = 9.68INKK127 pKa = 8.98INKK130 pKa = 8.7KK131 pKa = 10.02

Molecular weight:
14.71 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

227

0

227

75114

38

1464

330.9

38.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.481 ± 0.137

1.158 ± 0.048

4.023 ± 0.096

5.287 ± 0.142

5.412 ± 0.171

5.576 ± 0.149

1.401 ± 0.048

13.589 ± 0.18

12.641 ± 0.217

8.94 ± 0.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.06

8.434 ± 0.164

2.761 ± 0.063

1.886 ± 0.053

3.033 ± 0.095

6.703 ± 0.13

4.021 ± 0.078

4.049 ± 0.112

0.644 ± 0.063

5.2 ± 0.112

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski