Torque teno felis virus (isolate Fc-TTV4)
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q8QVL5|ORF3_TTVF1 Uncharacterized ORF3 protein OS=Torque teno felis virus (isolate Fc-TTV4) OX=766188 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.27 SASHH5 pKa = 6.67 SLSQSPNLHH14 pKa = 6.89 PDD16 pKa = 3.81 FTYY19 pKa = 10.66 KK20 pKa = 10.34 RR21 pKa = 11.84 QEE23 pKa = 4.18 ALWKK27 pKa = 9.26 QLVSAEE33 pKa = 3.95 HH34 pKa = 7.03 RR35 pKa = 11.84 KK36 pKa = 9.28 FCSCGDD42 pKa = 3.63 YY43 pKa = 9.33 TQHH46 pKa = 6.32 FRR48 pKa = 11.84 FPSPVKK54 pKa = 9.57 EE55 pKa = 4.22 DD56 pKa = 2.98 EE57 pKa = 4.7 CIRR60 pKa = 11.84 VVGEE64 pKa = 3.82 EE65 pKa = 4.25 GGDD68 pKa = 3.72 GVAVSYY74 pKa = 10.81 HH75 pKa = 4.19 VTKK78 pKa = 10.74 EE79 pKa = 4.17 SGDD82 pKa = 3.69 EE83 pKa = 4.02 DD84 pKa = 4.32 PEE86 pKa = 3.99 EE87 pKa = 4.42 VMASIAVGDD96 pKa = 4.93 DD97 pKa = 3.43 GDD99 pKa = 6.14 DD100 pKa = 4.61 DD101 pKa = 6.47 LEE103 pKa = 4.36 LCKK106 pKa = 10.3 WNLKK110 pKa = 9.96 PEE112 pKa = 4.06 PLILKK117 pKa = 8.02 QTDD120 pKa = 3.76 PQPLLTSSPGISTVMGSSKK139 pKa = 9.17 TRR141 pKa = 11.84 LMNEE145 pKa = 3.71 LLEE148 pKa = 4.96 IIHH151 pKa = 6.57 ALSGPDD157 pKa = 3.14 PWEE160 pKa = 4.19 SDD162 pKa = 2.93 GGMGPQGEE170 pKa = 4.25 PSKK173 pKa = 11.33 SNSRR177 pKa = 11.84 LLYY180 pKa = 10.22 SDD182 pKa = 5.25 PNPEE186 pKa = 4.77 DD187 pKa = 3.66 SSSDD191 pKa = 3.64 DD192 pKa = 3.58 SEE194 pKa = 3.9 MSYY197 pKa = 10.96 FSSSDD202 pKa = 3.3 STDD205 pKa = 3.37 ADD207 pKa = 2.88 ITYY210 pKa = 10.2 PNMEE214 pKa = 4.72 GGRR217 pKa = 11.84 TPPRR221 pKa = 11.84 YY222 pKa = 8.86 PPPNMGIYY230 pKa = 9.96 FF231 pKa = 4.25
Molecular weight: 25.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.35
IPC2_protein 4.355
IPC_protein 4.304
Toseland 4.139
ProMoST 4.431
Dawson 4.279
Bjellqvist 4.418
Wikipedia 4.164
Rodwell 4.151
Grimsley 4.037
Solomon 4.266
Lehninger 4.228
Nozaki 4.38
DTASelect 4.571
Thurlkill 4.151
EMBOSS 4.177
Sillero 4.431
Patrickios 3.516
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.357
Protein with the highest isoelectric point:
>sp|Q8QVL4|ORF2_TTVF1 Uncharacterized ORF2 protein OS=Torque teno felis virus (isolate Fc-TTV4) OX=766188 GN=ORF1 PE=4 SV=1
MM1 pKa = 6.26 YY2 pKa = 9.92 QSRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 WGRR14 pKa = 11.84 RR15 pKa = 11.84 ILSRR19 pKa = 11.84 YY20 pKa = 6.84 KK21 pKa = 10.41 RR22 pKa = 11.84 KK23 pKa = 8.48 WGRR26 pKa = 11.84 RR27 pKa = 11.84 SRR29 pKa = 11.84 RR30 pKa = 11.84 GHH32 pKa = 6.16 GIYY35 pKa = 10.12 RR36 pKa = 11.84 RR37 pKa = 11.84 WRR39 pKa = 11.84 RR40 pKa = 11.84 WRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 PRR46 pKa = 11.84 TVVTEE51 pKa = 3.7 QHH53 pKa = 5.15 SRR55 pKa = 11.84 RR56 pKa = 11.84 VKK58 pKa = 9.41 TIIVRR63 pKa = 11.84 GWEE66 pKa = 3.94 PLGNICPTDD75 pKa = 3.53 SARR78 pKa = 11.84 AKK80 pKa = 7.68 ATPYY84 pKa = 10.97 ASYY87 pKa = 11.07 DD88 pKa = 3.47 SDD90 pKa = 3.65 SGQGQWHH97 pKa = 5.87 GTWGHH102 pKa = 5.63 HH103 pKa = 4.66 WFTFQSLVDD112 pKa = 3.63 RR113 pKa = 11.84 AEE115 pKa = 3.8 ARR117 pKa = 11.84 LNSFSGNWEE126 pKa = 3.92 SYY128 pKa = 10.83 DD129 pKa = 3.5 YY130 pKa = 11.52 LRR132 pKa = 11.84 FLGGTMYY139 pKa = 10.77 FMQPRR144 pKa = 11.84 EE145 pKa = 3.87 MCFMFGNDD153 pKa = 4.22 PYY155 pKa = 11.86 LMTSDD160 pKa = 4.11 LDD162 pKa = 3.64 KK163 pKa = 10.85 TASQKK168 pKa = 10.16 NRR170 pKa = 11.84 AEE172 pKa = 4.35 EE173 pKa = 3.9 TWITPGYY180 pKa = 10.17 LMHH183 pKa = 7.46 RR184 pKa = 11.84 PGTHH188 pKa = 7.51 LILSRR193 pKa = 11.84 QKK195 pKa = 9.31 VEE197 pKa = 3.63 RR198 pKa = 11.84 RR199 pKa = 11.84 SMYY202 pKa = 10.04 KK203 pKa = 9.58 IRR205 pKa = 11.84 VPVPTSWRR213 pKa = 11.84 GWFPIPDD220 pKa = 3.76 CFSYY224 pKa = 10.51 VLCHH228 pKa = 6.97 WYY230 pKa = 6.88 WTWWDD235 pKa = 3.35 PDD237 pKa = 3.25 ACFFDD242 pKa = 4.19 PCATGSSCEE251 pKa = 3.99 AEE253 pKa = 4.53 PWWSTAQTKK262 pKa = 7.47 QAWVDD267 pKa = 3.47 RR268 pKa = 11.84 TKK270 pKa = 11.23 LDD272 pKa = 3.98 DD273 pKa = 4.44 PPVGGTGPNQKK284 pKa = 7.53 TWAPFLPSRR293 pKa = 11.84 PCTNYY298 pKa = 8.33 YY299 pKa = 8.02 THH301 pKa = 7.08 SASFWFKK308 pKa = 11.17 YY309 pKa = 9.82 KK310 pKa = 10.85 LKK312 pKa = 10.6 FQVTGEE318 pKa = 4.46 NIWAPVPRR326 pKa = 11.84 DD327 pKa = 3.19 YY328 pKa = 11.12 SQRR331 pKa = 11.84 GTVPTAPSRR340 pKa = 11.84 QQVEE344 pKa = 4.39 SEE346 pKa = 3.81 ARR348 pKa = 11.84 APYY351 pKa = 9.89 PKK353 pKa = 9.83 TNRR356 pKa = 11.84 PPTTADD362 pKa = 3.45 ILPGDD367 pKa = 4.5 LDD369 pKa = 3.8 SDD371 pKa = 4.78 GILEE375 pKa = 4.3 DD376 pKa = 3.44 EE377 pKa = 4.57 AYY379 pKa = 10.42 EE380 pKa = 5.11 RR381 pKa = 11.84 ITRR384 pKa = 11.84 DD385 pKa = 3.18 NPCPKK390 pKa = 9.95 RR391 pKa = 11.84 PRR393 pKa = 11.84 PLGIRR398 pKa = 11.84 WWDD401 pKa = 3.46 GTPGRR406 pKa = 11.84 TLQEE410 pKa = 3.87 QQQAAVLRR418 pKa = 11.84 PKK420 pKa = 10.19 PRR422 pKa = 11.84 RR423 pKa = 11.84 QLLRR427 pKa = 11.84 RR428 pKa = 11.84 LRR430 pKa = 11.84 DD431 pKa = 3.28 VLLQLL436 pKa = 4.64
Molecular weight: 51.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.604
IPC_protein 10.394
Toseland 10.248
ProMoST 10.072
Dawson 10.467
Bjellqvist 10.218
Wikipedia 10.701
Rodwell 10.599
Grimsley 10.555
Solomon 10.526
Lehninger 10.482
Nozaki 10.292
DTASelect 10.204
Thurlkill 10.321
EMBOSS 10.672
Sillero 10.394
Patrickios 10.058
IPC_peptide 10.526
IPC2_peptide 9.355
IPC2.peptide.svr19 8.52
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
772
105
436
257.3
29.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.793 ± 0.516
2.073 ± 0.21
7.254 ± 1.403
6.088 ± 1.79
3.238 ± 0.254
6.995 ± 0.436
2.591 ± 0.611
3.109 ± 0.311
4.145 ± 0.216
6.477 ± 0.348
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.332 ± 0.443
2.332 ± 0.383
8.29 ± 0.919
4.145 ± 0.514
8.938 ± 3.164
9.067 ± 2.022
5.829 ± 1.229
4.663 ± 1.05
3.756 ± 1.329
3.886 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here