Thermoplasmatales archaeon SCGC AB-539-N05

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales; unclassified Thermoplasmatales

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M7TCL2|M7TCL2_9ARCH Uncharacterized protein OS=Thermoplasmatales archaeon SCGC AB-539-N05 OX=1198116 GN=MBGDN05_00153 PE=4 SV=1
DDD2 pKa = 4.32LSNEEE7 pKa = 3.94TKKK10 pKa = 9.43IIVSNVPPVANFTWTPTNPNQKKK33 pKa = 10.16DDD35 pKa = 3.48VSFNSTTSYYY45 pKa = 11.43DD46 pKa = 3.53DDD48 pKa = 3.69FIVNWSWTFGDDD60 pKa = 4.25NTSYYY65 pKa = 10.47EE66 pKa = 4.12NPSHHH71 pKa = 7.56YYY73 pKa = 10.31DD74 pKa = 3.71DD75 pKa = 4.11GNYYY79 pKa = 7.54ATLTIEEE86 pKa = 5.15DD87 pKa = 4.2DDD89 pKa = 4.23SHHH92 pKa = 5.56TINKKK97 pKa = 7.55IQVNNTLPTVDDD109 pKa = 6.87NFTWTATYYY118 pKa = 9.7FSHHH122 pKa = 6.97TDDD125 pKa = 3.25DDD127 pKa = 3.38VNFTDDD133 pKa = 3.61SSDDD137 pKa = 3.59DDD139 pKa = 3.83EEE141 pKa = 4.4IAWHHH146 pKa = 6.57EEE148 pKa = 3.76SDDD151 pKa = 3.43HHH153 pKa = 7.44SDDD156 pKa = 3.54EE157 pKa = 4.54NPLHHH162 pKa = 6.72FIDDD166 pKa = 3.64SVYYY170 pKa = 10.53VTLTVWDDD178 pKa = 4.8DD179 pKa = 3.72GASNSTSKKK188 pKa = 10.96VTVVNIPPTADDD200 pKa = 3.18TYYY203 pKa = 9.04PHHH206 pKa = 6.78FGIFEEE212 pKa = 4.9DD213 pKa = 3.7HHH215 pKa = 5.82TDDD218 pKa = 4.73STDDD222 pKa = 3.18DDD224 pKa = 4.04VIVNWTWNFGDDD236 pKa = 4.19NISYYY241 pKa = 8.45EE242 pKa = 3.97NPVYYY247 pKa = 10.61YYY249 pKa = 10.74KKK251 pKa = 10.81GNFTITLNVTDDD263 pKa = 5.63DD264 pKa = 4.77DD265 pKa = 4.82ATDDD269 pKa = 3.41ATTQNITVLNNPLVADDD286 pKa = 4.41TWNPTNPTDDD296 pKa = 4.31EEE298 pKa = 4.69VNFTDDD304 pKa = 3.7SKKK307 pKa = 11.38DD308 pKa = 3.09DDD310 pKa = 3.68TVVNWTWNFGDDD322 pKa = 4.38NHHH325 pKa = 6.84YYY327 pKa = 8.43QHHH330 pKa = 6.63THHH333 pKa = 6.55YYY335 pKa = 10.77DDD337 pKa = 3.25GTYYY341 pKa = 9.95VTLTVRR347 pKa = 11.84DDD349 pKa = 3.99NDDD352 pKa = 3.04EE353 pKa = 4.54NTTSKKK359 pKa = 11.07DD360 pKa = 3.07TVLNVPPVADDD371 pKa = 4.12TYYY374 pKa = 10.71PTTPTNTDDD383 pKa = 3.72IQFNDDD389 pKa = 3.51STDDD393 pKa = 3.1DDD395 pKa = 3.76TIVSWSWDDD404 pKa = 3.47GDDD407 pKa = 5.44NTSTNQNPSHHH418 pKa = 6.45YYY420 pKa = 10.2AIGNYYY426 pKa = 8.0VTLTVTDDD434 pKa = 3.66DDD436 pKa = 4.09ATDDD440 pKa = 4.06SQKKK444 pKa = 8.68VNVSASPVAKKK455 pKa = 10.3IYYY458 pKa = 10.41PIAYYY463 pKa = 9.48NVNEEE468 pKa = 4.21IKKK471 pKa = 10.32LDDD474 pKa = 3.36SYYY477 pKa = 11.32DD478 pKa = 3.33DDD480 pKa = 4.57YYY482 pKa = 10.69MNWSWNFGDDD492 pKa = 5.03NHHH495 pKa = 6.46YYY497 pKa = 11.0EE498 pKa = 4.45KK499 pKa = 10.79DD500 pKa = 3.61IHHH503 pKa = 7.43YYY505 pKa = 8.31AANTYYY511 pKa = 8.51VMLTVRR517 pKa = 11.84DDD519 pKa = 3.67SGATNSTSKKK529 pKa = 10.89DD530 pKa = 3.34IVTT

Molecular weight: 59.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7U2Z1|M7U2Z1_9ARCH 30S ribosomal protein S2 OS=Thermoplasmatales archaeon SCGC AB-539-N05 OX=1198116 GN=rps2 PE=3 SV=1
QQ1 pKa = 6.66RR2 pKa = 11.84EE3 pKa = 3.63KK4 pKa = 10.7RR5 pKa = 11.84ARR7 pKa = 11.84TGAPMTYY14 pKa = 9.32TIHH17 pKa = 7.88DD18 pKa = 4.28KK19 pKa = 11.26GLSTTIGWQNRR30 pKa = 11.84DD31 pKa = 2.83AYY33 pKa = 10.37GRR35 pKa = 11.84SIPTRR40 pKa = 11.84NRR42 pKa = 11.84AQLYY46 pKa = 10.02RR47 pKa = 11.84LRR49 pKa = 11.84KK50 pKa = 6.68WQTRR54 pKa = 11.84TRR56 pKa = 11.84IGNATEE62 pKa = 4.03RR63 pKa = 11.84NLAIALSTIDD73 pKa = 3.91RR74 pKa = 11.84MSSGLSLPRR83 pKa = 11.84TVRR86 pKa = 11.84EE87 pKa = 4.05TAAMIYY93 pKa = 10.07RR94 pKa = 11.84KK95 pKa = 9.73AVLKK99 pKa = 10.63NLIRR103 pKa = 11.84GRR105 pKa = 11.84SVEE108 pKa = 4.15GVTAAALYY116 pKa = 8.71AACRR120 pKa = 11.84QCRR123 pKa = 11.84VPRR126 pKa = 11.84TLDD129 pKa = 3.74EE130 pKa = 4.4ISAIACMSRR139 pKa = 11.84KK140 pKa = 9.68EE141 pKa = 3.5IGRR144 pKa = 11.84NYY146 pKa = 10.15RR147 pKa = 11.84YY148 pKa = 9.68VARR151 pKa = 11.84EE152 pKa = 4.09LGLKK156 pKa = 10.12LMPTSPQDD164 pKa = 3.59YY165 pKa = 9.79ISRR168 pKa = 11.84FCSGLKK174 pKa = 10.52LSGDD178 pKa = 4.0VQAKK182 pKa = 8.31TLDD185 pKa = 3.63ILKK188 pKa = 9.99EE189 pKa = 3.85AADD192 pKa = 4.36RR193 pKa = 11.84EE194 pKa = 4.71LTSGRR199 pKa = 11.84GPTGIAAAALYY210 pKa = 9.97IATILCGEE218 pKa = 4.28RR219 pKa = 11.84RR220 pKa = 11.84TQRR223 pKa = 11.84EE224 pKa = 3.96VAEE227 pKa = 4.28VAGVTEE233 pKa = 3.89VTIRR237 pKa = 11.84NRR239 pKa = 11.84YY240 pKa = 9.01KK241 pKa = 10.51EE242 pKa = 4.18LSEE245 pKa = 4.18KK246 pKa = 10.87LDD248 pKa = 3.43INVILL253 pKa = 4.55

Molecular weight: 28.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

844

0

844

238614

20

3387

282.7

31.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.05 ± 0.08

1.356 ± 0.035

5.926 ± 0.111

6.58 ± 0.089

4.237 ± 0.081

7.141 ± 0.083

1.854 ± 0.035

8.726 ± 0.107

8.273 ± 0.169

8.279 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.055

5.128 ± 0.122

3.863 ± 0.047

2.454 ± 0.044

3.855 ± 0.081

6.147 ± 0.127

5.78 ± 0.171

6.867 ± 0.084

1.251 ± 0.059

3.781 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski