Sanxia water strider virus 17
Average proteome isoelectric point is 8.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KG99|A0A1L3KG99_9VIRU Capsid protein OS=Sanxia water strider virus 17 OX=1923401 PE=3 SV=1
MM1 pKa = 8.03 PINIRR6 pKa = 11.84 TPVTASPEE14 pKa = 4.12 QSSSHH19 pKa = 5.55 TPFFCWKK26 pKa = 9.22 VFNRR30 pKa = 11.84 VQRR33 pKa = 11.84 VTPKK37 pKa = 10.19 PKK39 pKa = 9.9 TSVATAEE46 pKa = 3.92 YY47 pKa = 10.52 RR48 pKa = 11.84 PGKK51 pKa = 9.33 VNLMQKK57 pKa = 8.45 TRR59 pKa = 11.84 DD60 pKa = 3.23 RR61 pKa = 11.84 FFKK64 pKa = 9.35 MWNGNDD70 pKa = 3.11 LPKK73 pKa = 10.14 RR74 pKa = 11.84 SKK76 pKa = 9.79 YY77 pKa = 10.37 ASNRR81 pKa = 11.84 RR82 pKa = 11.84 SLPPIDD88 pKa = 3.97 FRR90 pKa = 11.84 KK91 pKa = 9.3 MLHH94 pKa = 6.13 HH95 pKa = 6.68 HH96 pKa = 6.92 LKK98 pKa = 10.34 SIPKK102 pKa = 9.37 IPMASNHH109 pKa = 4.88 THH111 pKa = 6.77 PNAASLRR118 pKa = 11.84 TSVAVEE124 pKa = 3.7 LEE126 pKa = 4.21 KK127 pKa = 10.69 IVINAGFRR135 pKa = 11.84 PYY137 pKa = 10.26 SVSMSKK143 pKa = 10.01 RR144 pKa = 11.84 DD145 pKa = 3.42 QYY147 pKa = 11.6 DD148 pKa = 3.08 GCRR151 pKa = 11.84 YY152 pKa = 10.42 YY153 pKa = 11.31 FMDD156 pKa = 5.05 KK157 pKa = 10.99 DD158 pKa = 3.43 LDD160 pKa = 3.57 KK161 pKa = 11.35 AFRR164 pKa = 11.84 DD165 pKa = 3.6 DD166 pKa = 4.31 RR167 pKa = 11.84 VTEE170 pKa = 3.97 EE171 pKa = 4.66 HH172 pKa = 6.53 VLLMIDD178 pKa = 2.82 VDD180 pKa = 4.4 YY181 pKa = 11.62 YY182 pKa = 11.7 CDD184 pKa = 3.41 INQYY188 pKa = 10.05 LQLGNPIMIYY198 pKa = 9.57 TFVPTEE204 pKa = 3.83 AGGRR208 pKa = 11.84 ALDD211 pKa = 3.93 ASYY214 pKa = 10.07 TINNNVVSYY223 pKa = 9.36 CVKK226 pKa = 10.66 GGATYY231 pKa = 10.1 HH232 pKa = 6.41 HH233 pKa = 6.84 EE234 pKa = 3.88 LWDD237 pKa = 3.81 YY238 pKa = 11.37 QGDD241 pKa = 3.96 NVVVKK246 pKa = 10.3 DD247 pKa = 3.39 KK248 pKa = 11.23 HH249 pKa = 6.68 GNTLVYY255 pKa = 10.53 VIEE258 pKa = 3.9 QHH260 pKa = 7.18 IIEE263 pKa = 4.84 EE264 pKa = 4.4 DD265 pKa = 3.28 PNRR268 pKa = 11.84 RR269 pKa = 11.84 VIGFYY274 pKa = 10.49 PIASYY279 pKa = 9.98 PKK281 pKa = 8.19 HH282 pKa = 5.41 TCPFKK287 pKa = 10.42 PEE289 pKa = 4.04 SYY291 pKa = 10.76 GFGRR295 pKa = 11.84 LRR297 pKa = 11.84 PSSMGVNCIRR307 pKa = 11.84 NVTDD311 pKa = 5.07 DD312 pKa = 4.23 IISVAVQDD320 pKa = 3.69 SANAVTIPQSIYY332 pKa = 10.02 DD333 pKa = 3.74 ALVVRR338 pKa = 11.84 RR339 pKa = 11.84 GEE341 pKa = 4.21 SKK343 pKa = 10.83 NPVIADD349 pKa = 3.17 VEE351 pKa = 4.55 RR352 pKa = 11.84 ILNAEE357 pKa = 4.56 HH358 pKa = 7.04 IEE360 pKa = 4.04 HH361 pKa = 6.47 SAIKK365 pKa = 10.59 APVLFKK371 pKa = 10.91 LLQSSTTSCGVITSSATIGQAKK393 pKa = 9.74 NFQTLFPLATEE404 pKa = 4.56 DD405 pKa = 3.6 GKK407 pKa = 10.26 PVGRR411 pKa = 11.84 AVAPSLVTDD420 pKa = 4.26 PAFVPCKK427 pKa = 10.6 SFNNDD432 pKa = 2.38 NATIQGRR439 pKa = 11.84 VNKK442 pKa = 9.42 VRR444 pKa = 11.84 NNNQSPPAWKK454 pKa = 8.95 TYY456 pKa = 9.95 DD457 pKa = 3.33 AEE459 pKa = 4.27 LVKK462 pKa = 10.6 FIVPHH467 pKa = 6.03 NKK469 pKa = 9.45 NATGVPFDD477 pKa = 3.76 YY478 pKa = 10.95 EE479 pKa = 4.32 RR480 pKa = 11.84 VIEE483 pKa = 4.29 LQNKK487 pKa = 6.24 PAQRR491 pKa = 11.84 GRR493 pKa = 11.84 SEE495 pKa = 3.89 QAKK498 pKa = 8.58 ATVSNDD504 pKa = 3.03 YY505 pKa = 10.54 VNKK508 pKa = 9.83 VKK510 pKa = 11.01 AFIKK514 pKa = 10.36 AEE516 pKa = 4.2 AYY518 pKa = 10.44 ASVTDD523 pKa = 3.92 PRR525 pKa = 11.84 NISTVDD531 pKa = 3.24 VSHH534 pKa = 6.18 QLSYY538 pKa = 11.68 SRR540 pKa = 11.84 FTLPFKK546 pKa = 10.45 IDD548 pKa = 3.53 CLKK551 pKa = 9.6 DD552 pKa = 3.61 QKK554 pKa = 10.49 WFASSMTPKK563 pKa = 10.18 EE564 pKa = 3.52 ISDD567 pKa = 3.5 RR568 pKa = 11.84 VMEE571 pKa = 4.5 ICQYY575 pKa = 8.1 PHH577 pKa = 7.02 GIIVSDD583 pKa = 3.62 YY584 pKa = 10.73 SRR586 pKa = 11.84 LDD588 pKa = 3.43 GHH590 pKa = 6.91 VSAADD595 pKa = 3.46 KK596 pKa = 10.96 AFKK599 pKa = 10.1 EE600 pKa = 4.07 AVYY603 pKa = 10.29 QSWCAHH609 pKa = 7.04 AYY611 pKa = 9.63 RR612 pKa = 11.84 AEE614 pKa = 4.03 LSKK617 pKa = 10.86 ILAADD622 pKa = 4.42 RR623 pKa = 11.84 NPKK626 pKa = 10.06 GVTAQGLKK634 pKa = 10.06 YY635 pKa = 10.64 DD636 pKa = 4.2 PGYY639 pKa = 10.24 SQLSGSPGTTNDD651 pKa = 3.37 NNLVTLRR658 pKa = 11.84 HH659 pKa = 6.56 DD660 pKa = 4.22 YY661 pKa = 10.52 IALRR665 pKa = 11.84 EE666 pKa = 4.15 LGNTPKK672 pKa = 10.51 QAWQLVQQWVLGASDD687 pKa = 3.5 DD688 pKa = 4.34 RR689 pKa = 11.84 IRR691 pKa = 11.84 ANIPGLAQKK700 pKa = 10.74 LEE702 pKa = 4.16 EE703 pKa = 4.21 VATKK707 pKa = 10.45 LGHH710 pKa = 5.0 QLKK713 pKa = 10.66 SIILEE718 pKa = 4.08 PLAGCPVPFLGRR730 pKa = 11.84 IYY732 pKa = 10.82 ASPATHH738 pKa = 6.77 NDD740 pKa = 3.02 SVQDD744 pKa = 3.87 PEE746 pKa = 3.97 RR747 pKa = 11.84 TLAKK751 pKa = 10.28 LHH753 pKa = 6.1 LTMSPPTITPLQALYY768 pKa = 10.57 NRR770 pKa = 11.84 AYY772 pKa = 10.26 GYY774 pKa = 11.12 YY775 pKa = 8.68 VTDD778 pKa = 4.13 CKK780 pKa = 10.78 TPIIGTWCRR789 pKa = 11.84 RR790 pKa = 11.84 VIEE793 pKa = 4.02 ILEE796 pKa = 4.28 TQGMEE801 pKa = 3.83 LRR803 pKa = 11.84 QATGEE808 pKa = 3.54 EE809 pKa = 4.58 DD810 pKa = 3.51 YY811 pKa = 10.71 RR812 pKa = 11.84 IKK814 pKa = 10.72 SGPYY818 pKa = 7.42 PQEE821 pKa = 3.82 NEE823 pKa = 3.76 EE824 pKa = 4.13 LLRR827 pKa = 11.84 NLMCQLLDD835 pKa = 3.68 LTADD839 pKa = 3.51 EE840 pKa = 5.06 LDD842 pKa = 4.02 SIEE845 pKa = 4.3 RR846 pKa = 11.84 AINSATTVEE855 pKa = 4.65 DD856 pKa = 3.76 LPEE859 pKa = 5.43 AILDD863 pKa = 3.71 NGHH866 pKa = 6.61 TIRR869 pKa = 11.84 HH870 pKa = 6.34 KK871 pKa = 9.85 INAAVGHH878 pKa = 7.29 DD879 pKa = 3.33 ILGPVPSVSSEE890 pKa = 3.78 TSEE893 pKa = 3.92 PKK895 pKa = 10.3 VEE897 pKa = 4.55 EE898 pKa = 4.21 EE899 pKa = 4.4 PKK901 pKa = 10.59 CPSVPIISEE910 pKa = 4.33 TSASPEE916 pKa = 4.12 MIGSDD921 pKa = 3.13 ASNPISASPSPPPATATNISHH942 pKa = 6.66 PTAGTRR948 pKa = 11.84 KK949 pKa = 9.55 PSINKK954 pKa = 9.2 QSSRR958 pKa = 11.84 QLKK961 pKa = 8.82 QSSPRR966 pKa = 11.84 SQQTKK971 pKa = 8.06 HH972 pKa = 5.65 QSVNLTPRR980 pKa = 11.84 THH982 pKa = 6.1 HH983 pKa = 6.38 TFNEE987 pKa = 4.52 CTRR990 pKa = 11.84 KK991 pKa = 10.02 DD992 pKa = 2.95 ACRR995 pKa = 11.84 YY996 pKa = 8.17 HH997 pKa = 7.23 RR998 pKa = 11.84 NGATKK1003 pKa = 9.95 PKK1005 pKa = 9.06 QTTAPRR1011 pKa = 11.84 KK1012 pKa = 9.23
Molecular weight: 112.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.75
IPC2_protein 8.009
IPC_protein 7.819
Toseland 7.688
ProMoST 8.463
Dawson 8.624
Bjellqvist 8.843
Wikipedia 8.609
Rodwell 8.682
Grimsley 7.761
Solomon 8.668
Lehninger 8.668
Nozaki 8.975
DTASelect 8.624
Thurlkill 8.697
EMBOSS 8.799
Sillero 8.96
Patrickios 4.393
IPC_peptide 8.668
IPC2_peptide 7.658
IPC2.peptide.svr19 7.878
Protein with the highest isoelectric point:
>tr|A0A1L3KG99|A0A1L3KG99_9VIRU Capsid protein OS=Sanxia water strider virus 17 OX=1923401 PE=3 SV=1
MM1 pKa = 7.92 APQNQNKK8 pKa = 7.12 QQPRR12 pKa = 11.84 ASKK15 pKa = 10.23 RR16 pKa = 11.84 SQRR19 pKa = 11.84 TNMNQQRR26 pKa = 11.84 TVASVPRR33 pKa = 11.84 QGLRR37 pKa = 11.84 SKK39 pKa = 10.14 QQTYY43 pKa = 7.26 MTGQPKK49 pKa = 9.41 FQHH52 pKa = 6.56 KK53 pKa = 8.99 GDD55 pKa = 3.66 KK56 pKa = 10.75 VIVSNSEE63 pKa = 3.41 ILVEE67 pKa = 4.33 VNGTVASGVIPAGGAIRR84 pKa = 11.84 VFRR87 pKa = 11.84 FEE89 pKa = 4.89 NVATGNNMNTTRR101 pKa = 11.84 WLTGMAKK108 pKa = 10.3 LYY110 pKa = 11.09 DD111 pKa = 3.58 KK112 pKa = 11.14 FRR114 pKa = 11.84 VNKK117 pKa = 9.73 MNMRR121 pKa = 11.84 WVTSVPFTYY130 pKa = 10.23 GGQVALRR137 pKa = 11.84 FDD139 pKa = 4.19 SDD141 pKa = 3.82 PSKK144 pKa = 8.39 TTADD148 pKa = 3.37 ASLLAVSGDD157 pKa = 3.33 MRR159 pKa = 11.84 ALATAVYY166 pKa = 9.69 NSASNRR172 pKa = 11.84 VKK174 pKa = 10.6 KK175 pKa = 10.48 DD176 pKa = 3.02 QLNRR180 pKa = 11.84 LPQYY184 pKa = 7.97 EE185 pKa = 4.54 TFPSATDD192 pKa = 3.21 TGVATVGSINLAYY205 pKa = 10.51 SAITPPAGTTGTINLGYY222 pKa = 10.2 VWVDD226 pKa = 3.06 YY227 pKa = 10.59 EE228 pKa = 4.76 VEE230 pKa = 4.31 FFNPSNAIAAA240 pKa = 4.21
Molecular weight: 26.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.218
IPC2_protein 9.575
IPC_protein 9.838
Toseland 10.218
ProMoST 9.897
Dawson 10.394
Bjellqvist 10.072
Wikipedia 10.584
Rodwell 10.774
Grimsley 10.467
Solomon 10.438
Lehninger 10.394
Nozaki 10.175
DTASelect 10.072
Thurlkill 10.248
EMBOSS 10.613
Sillero 10.306
Patrickios 10.423
IPC_peptide 10.438
IPC2_peptide 8.668
IPC2.peptide.svr19 8.716
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1252
240
1012
626.0
69.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.227 ± 0.843
1.438 ± 0.684
5.112 ± 0.648
4.553 ± 0.976
2.875 ± 0.416
5.112 ± 0.938
2.556 ± 1.017
5.671 ± 1.112
5.911 ± 0.433
6.23 ± 0.585
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.917 ± 0.674
5.911 ± 0.954
6.869 ± 0.889
4.633 ± 0.769
5.911 ± 0.161
7.907 ± 0.194
7.109 ± 0.979
7.348 ± 0.865
0.958 ± 0.139
3.754 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here