Candidatus Poribacteria bacterium

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Poribacteria

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3447 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A496AP14|A0A496AP14_9BACT Uncharacterized protein OS=Candidatus Poribacteria bacterium OX=2026781 GN=C6497_08150 PE=4 SV=1
MM1 pKa = 7.9NMISEE6 pKa = 4.62LKK8 pKa = 10.56ARR10 pKa = 11.84IKK12 pKa = 10.09IQCLSLIFITGTLIILFTIVGCSDD36 pKa = 3.0TSTPYY41 pKa = 10.05TGTMPTSIDD50 pKa = 3.36VSDD53 pKa = 3.88YY54 pKa = 11.2AISNTGGLYY63 pKa = 10.45CIYY66 pKa = 10.35NDD68 pKa = 4.27TGSSCIDD75 pKa = 4.85LIPLISTGSVDD86 pKa = 3.52EE87 pKa = 4.52GPIVHH92 pKa = 7.32IYY94 pKa = 9.41PEE96 pKa = 3.86RR97 pKa = 11.84TLYY100 pKa = 10.73LFYY103 pKa = 11.46YY104 pKa = 8.76EE105 pKa = 4.58GKK107 pKa = 9.63PIIRR111 pKa = 11.84AEE113 pKa = 4.11RR114 pKa = 11.84VGDD117 pKa = 3.43TTEE120 pKa = 3.99LVKK123 pKa = 10.27TLVDD127 pKa = 4.14RR128 pKa = 11.84DD129 pKa = 3.32GDD131 pKa = 4.04LPNEE135 pKa = 4.47KK136 pKa = 8.77PTSPGGAGNNPNNNNEE152 pKa = 4.1SDD154 pKa = 4.19GGDD157 pKa = 3.53SNNNNPNGNGDD168 pKa = 3.65SDD170 pKa = 4.8GGDD173 pKa = 3.48SNDD176 pKa = 4.15NNPNEE181 pKa = 4.53NNDD184 pKa = 3.36IDD186 pKa = 5.46DD187 pKa = 5.41DD188 pKa = 4.38STGDD192 pKa = 3.39NTDD195 pKa = 3.35AEE197 pKa = 4.76EE198 pKa = 4.62EE199 pKa = 4.24DD200 pKa = 4.38SSDD203 pKa = 3.28NTGRR207 pKa = 11.84TDD209 pKa = 2.92NRR211 pKa = 11.84RR212 pKa = 11.84DD213 pKa = 3.45GFGWLIWIYY222 pKa = 10.31YY223 pKa = 9.34PEE225 pKa = 4.56GTLPIDD231 pKa = 3.71SPTLSEE237 pKa = 4.42SEE239 pKa = 4.08VTISINGKK247 pKa = 7.78QLSDD251 pKa = 3.82DD252 pKa = 5.3DD253 pKa = 3.67ITGFAKK259 pKa = 10.51FIEE262 pKa = 4.9SNGEE266 pKa = 3.71TGIQFYY272 pKa = 10.96YY273 pKa = 8.08PTEE276 pKa = 4.01SAEE279 pKa = 4.55LFDD282 pKa = 6.61LNLQIEE288 pKa = 4.78GVSYY292 pKa = 11.62SNGEE296 pKa = 3.88VKK298 pKa = 10.65FNINYY303 pKa = 9.64LWRR306 pKa = 11.84SDD308 pKa = 3.23

Molecular weight: 33.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A496AQM9|A0A496AQM9_9BACT LamGL domain-containing protein OS=Candidatus Poribacteria bacterium OX=2026781 GN=C6497_08125 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.93QPHH8 pKa = 5.25RR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 10.22RR12 pKa = 11.84KK13 pKa = 8.48NKK15 pKa = 9.05LGFRR19 pKa = 11.84KK20 pKa = 9.96RR21 pKa = 11.84MSTRR25 pKa = 11.84HH26 pKa = 4.55GRR28 pKa = 11.84KK29 pKa = 7.43TIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84RR40 pKa = 11.84VLSAA44 pKa = 3.76

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3447

0

3447

1197355

32

3094

347.4

39.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.33 ± 0.04

1.011 ± 0.014

6.175 ± 0.035

6.803 ± 0.042

4.203 ± 0.032

6.974 ± 0.036

2.322 ± 0.02

7.994 ± 0.035

5.279 ± 0.03

9.193 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.017

4.94 ± 0.025

4.351 ± 0.03

3.692 ± 0.027

4.767 ± 0.027

6.356 ± 0.031

6.174 ± 0.035

6.34 ± 0.027

1.429 ± 0.02

3.549 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski