Blastococcus sp. CT_GayMR16

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4365 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9QC28|A0A4Y9QC28_9ACTN 30S ribosomal protein S14 type Z OS=Blastococcus sp. CT_GayMR16 OX=2559607 GN=rpsZ PE=3 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84EE3 pKa = 3.97ALHH6 pKa = 6.43VKK8 pKa = 10.0LSKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.78SSLVATAIAAAMLMTACGGSDD34 pKa = 3.48DD35 pKa = 5.12SGDD38 pKa = 3.45GGAASDD44 pKa = 3.89GGSFSIYY51 pKa = 9.67IGEE54 pKa = 4.27PEE56 pKa = 4.07NPIVPGNTSEE66 pKa = 4.82SEE68 pKa = 4.06GSQVVEE74 pKa = 4.27ALWTGLVQYY83 pKa = 11.03ADD85 pKa = 4.31DD86 pKa = 5.04SSVQYY91 pKa = 10.34TGVAEE96 pKa = 4.49SIEE99 pKa = 4.38SDD101 pKa = 4.5DD102 pKa = 3.6ATTWTVKK109 pKa = 10.8LKK111 pKa = 10.87DD112 pKa = 2.95GWTFHH117 pKa = 7.56DD118 pKa = 4.4GTPVTAASFVDD129 pKa = 3.44AWNYY133 pKa = 7.46TALSTNAQGGSYY145 pKa = 10.03FFANVEE151 pKa = 4.45GYY153 pKa = 9.75DD154 pKa = 3.63ALQAEE159 pKa = 4.71TDD161 pKa = 3.57DD162 pKa = 4.93DD163 pKa = 5.35GNVITPPAAEE173 pKa = 4.22EE174 pKa = 3.99MTGLEE179 pKa = 4.26VVDD182 pKa = 4.51DD183 pKa = 3.75QTFTVTLTAPFAQYY197 pKa = 10.45PVTVGYY203 pKa = 10.18NAFYY207 pKa = 9.88PLPEE211 pKa = 4.51SFFEE215 pKa = 4.58DD216 pKa = 3.92PEE218 pKa = 4.66AAGKK222 pKa = 8.13TPVGNGPFKK231 pKa = 10.99ADD233 pKa = 3.35GEE235 pKa = 4.49FVPGQGFTLSRR246 pKa = 11.84YY247 pKa = 9.99DD248 pKa = 4.06DD249 pKa = 3.96YY250 pKa = 11.91AGEE253 pKa = 4.56DD254 pKa = 3.31AAQADD259 pKa = 4.04SVEE262 pKa = 4.46FKK264 pKa = 11.02VYY266 pKa = 10.6TDD268 pKa = 3.19INTAYY273 pKa = 9.8TDD275 pKa = 3.75TQGGNLDD282 pKa = 3.87ILNNIPPDD290 pKa = 4.8AISSAPDD297 pKa = 3.3EE298 pKa = 4.69FGDD301 pKa = 4.54RR302 pKa = 11.84YY303 pKa = 11.08GEE305 pKa = 4.29TPSSSFTYY313 pKa = 9.78MGFPTYY319 pKa = 10.55DD320 pKa = 2.96PRR322 pKa = 11.84YY323 pKa = 7.51ADD325 pKa = 3.09KK326 pKa = 10.68RR327 pKa = 11.84VRR329 pKa = 11.84QAFSLAIDD337 pKa = 3.79RR338 pKa = 11.84EE339 pKa = 4.6AITEE343 pKa = 4.51AIFNGTRR350 pKa = 11.84APAFSVIAPVVDD362 pKa = 4.57GSRR365 pKa = 11.84DD366 pKa = 3.65DD367 pKa = 3.67ACQYY371 pKa = 10.09CQVDD375 pKa = 3.54VEE377 pKa = 4.55RR378 pKa = 11.84ANEE381 pKa = 4.0LLDD384 pKa = 3.51EE385 pKa = 5.03AGFDD389 pKa = 3.36RR390 pKa = 11.84TQPVDD395 pKa = 2.84LWFNAGAGHH404 pKa = 6.64DD405 pKa = 3.6AWVEE409 pKa = 3.78AVGNQLRR416 pKa = 11.84EE417 pKa = 3.96NLGVEE422 pKa = 4.65FTLQGNLDD430 pKa = 3.61FSEE433 pKa = 4.17YY434 pKa = 10.96LPLGDD439 pKa = 3.69AKK441 pKa = 11.08GYY443 pKa = 8.02TGPFRR448 pKa = 11.84LGWSMDD454 pKa = 3.44YY455 pKa = 10.51PSPQNYY461 pKa = 9.97LEE463 pKa = 4.5PLYY466 pKa = 9.99STQAQPPAGSNSAFYY481 pKa = 11.16SNPEE485 pKa = 3.3FDD487 pKa = 4.29ALVAEE492 pKa = 5.16GNAASSNDD500 pKa = 3.51EE501 pKa = 5.34AIDD504 pKa = 4.58LYY506 pKa = 11.01QQAEE510 pKa = 4.21DD511 pKa = 4.84LLLEE515 pKa = 4.96DD516 pKa = 4.41MPIMPMFFGLEE527 pKa = 3.66QTVWSEE533 pKa = 3.78NVSDD537 pKa = 3.89VKK539 pKa = 11.2VDD541 pKa = 2.69IFGRR545 pKa = 11.84VNVASVTVKK554 pKa = 10.8

Molecular weight: 59.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9PXA5|A0A4Y9PXA5_9ACTN ANTAR domain-containing protein OS=Blastococcus sp. CT_GayMR16 OX=2559607 GN=E4P38_20755 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4365

0

4365

1348885

32

1976

309.0

32.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.88 ± 0.059

0.677 ± 0.01

6.372 ± 0.03

5.602 ± 0.038

2.67 ± 0.021

9.499 ± 0.038

1.975 ± 0.018

3.196 ± 0.026

1.578 ± 0.025

10.601 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.699 ± 0.013

1.618 ± 0.02

6.059 ± 0.03

2.77 ± 0.02

7.87 ± 0.047

5.142 ± 0.026

6.022 ± 0.031

9.497 ± 0.033

1.485 ± 0.016

1.789 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski