Thermoclostridium caenicola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Thermoclostridium

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6AN68|A0A1M6AN68_9FIRM Uncharacterized protein OS=Thermoclostridium caenicola OX=659425 GN=SAMN05444373_1001101 PE=4 SV=1
MM1 pKa = 6.24EE2 pKa = 4.87TKK4 pKa = 9.35RR5 pKa = 11.84TSLWYY10 pKa = 9.69QGKK13 pKa = 8.59HH14 pKa = 5.46ALSLLLVLIMILTMLPAIPAVADD37 pKa = 3.65DD38 pKa = 4.66TPDD41 pKa = 3.38DD42 pKa = 4.26VYY44 pKa = 11.38SGEE47 pKa = 4.58FMAEE51 pKa = 3.92SQQADD56 pKa = 4.32GEE58 pKa = 4.63SQDD61 pKa = 5.56DD62 pKa = 3.61PDD64 pKa = 3.98EE65 pKa = 5.03RR66 pKa = 11.84KK67 pKa = 10.47ASDD70 pKa = 3.67NPVLTTSAGEE80 pKa = 4.23TGKK83 pKa = 8.73DD84 pKa = 3.15TQEE87 pKa = 4.25EE88 pKa = 4.24YY89 pKa = 10.99DD90 pKa = 4.43EE91 pKa = 5.17EE92 pKa = 4.68VTTCDD97 pKa = 3.65AIEE100 pKa = 4.14VKK102 pKa = 10.26IEE104 pKa = 3.5NHH106 pKa = 5.86AAGNLATEE114 pKa = 4.35IEE116 pKa = 4.53AYY118 pKa = 9.91LDD120 pKa = 4.54AMGCPDD126 pKa = 3.99AYY128 pKa = 10.89GCIFSLKK135 pKa = 9.99VSGGEE140 pKa = 4.46LSKK143 pKa = 11.11ADD145 pKa = 3.61QDD147 pKa = 4.1YY148 pKa = 11.15LRR150 pKa = 11.84QTFWYY155 pKa = 9.21KK156 pKa = 10.45CDD158 pKa = 3.64YY159 pKa = 11.24LEE161 pKa = 6.15DD162 pKa = 3.79VDD164 pKa = 6.95FSDD167 pKa = 3.91TSLAYY172 pKa = 10.24DD173 pKa = 3.74EE174 pKa = 5.35GIEE177 pKa = 3.9MRR179 pKa = 11.84PGLFEE184 pKa = 3.85STSIIKK190 pKa = 10.24IKK192 pKa = 10.95LPDD195 pKa = 3.86NLTAISDD202 pKa = 3.84AAFSGSIFLAIVEE215 pKa = 4.3IPEE218 pKa = 4.29SVTSIGAGAFLGCEE232 pKa = 4.0CLLAIDD238 pKa = 4.69LPEE241 pKa = 4.04MPYY244 pKa = 10.96LLRR247 pKa = 11.84SGAISS252 pKa = 3.14

Molecular weight: 27.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6JDS3|A0A1M6JDS3_9FIRM Uncharacterized protein OS=Thermoclostridium caenicola OX=659425 GN=SAMN05444373_105526 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.46KK9 pKa = 8.28RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.76RR21 pKa = 11.84MKK23 pKa = 8.31TANGRR28 pKa = 11.84KK29 pKa = 7.56VLKK32 pKa = 10.22RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2603

0

2603

834208

29

1679

320.5

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.902 ± 0.049

1.259 ± 0.018

5.57 ± 0.035

7.157 ± 0.051

4.071 ± 0.034

7.301 ± 0.048

1.768 ± 0.019

7.594 ± 0.038

6.005 ± 0.041

9.579 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.885 ± 0.02

4.027 ± 0.031

3.883 ± 0.028

2.964 ± 0.024

5.671 ± 0.043

5.739 ± 0.034

4.987 ± 0.03

7.024 ± 0.036

0.914 ± 0.015

3.701 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski