Sulfolobus spindle-shaped virus 4

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8TKK9|A8TKK9_9VIRU Uncharacterized protein OS=Sulfolobus spindle-shaped virus 4 OX=459290 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84KK3 pKa = 9.01EE4 pKa = 3.92LLSLTFLALLLIPTLGILPSAQTSPNTWVIAFYY37 pKa = 10.29PGSYY41 pKa = 10.05PSGTSPSNFTSFTVNFTIPPFLQTSNGYY69 pKa = 10.32LGFVLTATVPATEE82 pKa = 4.55YY83 pKa = 10.54IGGLYY88 pKa = 10.35SGTVDD93 pKa = 2.85IALQIGLIFVTQTNTYY109 pKa = 10.34VLTAQVWTPYY119 pKa = 8.52GTQIYY124 pKa = 9.78GYY126 pKa = 10.33AEE128 pKa = 4.56TIYY131 pKa = 11.07LPANTEE137 pKa = 4.03AVISLIYY144 pKa = 10.53NYY146 pKa = 11.1ANGHH150 pKa = 4.51VAIGLLQGGVTVYY163 pKa = 10.82NVYY166 pKa = 10.35FPTLYY171 pKa = 10.51SHH173 pKa = 6.72NNCVYY178 pKa = 10.66NVTNNGGFSEE188 pKa = 4.6PSGQNPSFAIEE199 pKa = 4.03GTYY202 pKa = 9.77TGSPPFNDD210 pKa = 3.9TGWDD214 pKa = 3.81GMWFDD219 pKa = 4.96GAPISDD225 pKa = 3.49INAMTGYY232 pKa = 10.6SSATGVGGTASIPPYY247 pKa = 9.29YY248 pKa = 10.41AEE250 pKa = 4.34SGLTLFHH257 pKa = 6.8NVLFNYY263 pKa = 8.85WYY265 pKa = 10.82NPYY268 pKa = 10.61AWYY271 pKa = 9.73FSIGTPQDD279 pKa = 3.57YY280 pKa = 10.19SAHH283 pKa = 5.7GLHH286 pKa = 6.76YY287 pKa = 10.41IQLSEE292 pKa = 3.9GKK294 pKa = 9.74EE295 pKa = 3.86YY296 pKa = 9.94NTINGLNYY304 pKa = 9.99ALQWVPPP311 pKa = 4.15

Molecular weight: 33.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8TKG6|A8TKG6_9VIRU Uncharacterized protein OS=Sulfolobus spindle-shaped virus 4 OX=459290 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84WMSNGLTSPKK12 pKa = 10.01NIRR15 pKa = 11.84RR16 pKa = 11.84NSPRR20 pKa = 11.84FRR22 pKa = 11.84YY23 pKa = 9.7NYY25 pKa = 10.19YY26 pKa = 10.78DD27 pKa = 3.83YY28 pKa = 10.73LTSLQKK34 pKa = 10.92AKK36 pKa = 10.6AQSRR40 pKa = 11.84EE41 pKa = 4.01INIKK45 pKa = 8.66SQIHH49 pKa = 4.76RR50 pKa = 11.84VVYY53 pKa = 9.76RR54 pKa = 11.84HH55 pKa = 5.69FVSSRR60 pKa = 11.84LNRR63 pKa = 11.84KK64 pKa = 7.66SLCFLCVFSEE74 pKa = 4.24LLHH77 pKa = 6.63GSVPP81 pKa = 3.59

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

5005

45

808

147.2

16.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.775 ± 0.355

0.719 ± 0.252

3.057 ± 0.455

5.914 ± 1.07

4.555 ± 0.317

5.694 ± 0.682

1.279 ± 0.295

8.092 ± 0.434

7.353 ± 1.012

11.508 ± 0.643

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.269

5.315 ± 0.456

3.916 ± 0.527

3.217 ± 0.379

3.836 ± 0.742

7.373 ± 0.693

6.593 ± 0.699

7.293 ± 0.497

1.479 ± 0.225

5.854 ± 0.501
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski