Enterococcus pallens ATCC BAA-351

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus pallens

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R2SC12|R2SC12_9ENTE DNA translocase FtsK OS=Enterococcus pallens ATCC BAA-351 OX=1158607 GN=UAU_02702 PE=4 SV=1
MM1 pKa = 7.09YY2 pKa = 10.42KK3 pKa = 10.13SGKK6 pKa = 8.46HH7 pKa = 4.61WMFATITAFTGVLGASTLVTTEE29 pKa = 3.9AQATSASQDD38 pKa = 3.57DD39 pKa = 4.06AGMEE43 pKa = 4.13IDD45 pKa = 3.64TGTDD49 pKa = 2.78QVLANQEE56 pKa = 4.28TASIPAPEE64 pKa = 4.18TQEE67 pKa = 4.23AVNPDD72 pKa = 3.74PVVDD76 pKa = 3.89NTPEE80 pKa = 3.87SEE82 pKa = 4.34APVSEE87 pKa = 4.2IASEE91 pKa = 4.48SVVQTEE97 pKa = 4.66LEE99 pKa = 4.29STTEE103 pKa = 4.08STSTTEE109 pKa = 4.21SEE111 pKa = 4.64SNMDD115 pKa = 3.44SASISEE121 pKa = 4.34SVSVSEE127 pKa = 4.67SMSISEE133 pKa = 4.95SEE135 pKa = 4.21SSSQSRR141 pKa = 11.84SEE143 pKa = 4.04EE144 pKa = 3.97TSLSISTSEE153 pKa = 4.11STSNSALAEE162 pKa = 4.37VNNSASNSLSEE173 pKa = 4.41STSHH177 pKa = 6.71SEE179 pKa = 4.66SASTWVSNSLSEE191 pKa = 4.25STSNSEE197 pKa = 3.42NRR199 pKa = 11.84SAVNSLNKK207 pKa = 9.97EE208 pKa = 3.84KK209 pKa = 10.84SKK211 pKa = 11.05QPTEE215 pKa = 4.28KK216 pKa = 10.18EE217 pKa = 3.68QSANEE222 pKa = 3.96TNKK225 pKa = 10.74AKK227 pKa = 10.71DD228 pKa = 3.71LFDD231 pKa = 4.29QIVPDD236 pKa = 3.83GTEE239 pKa = 3.74VKK241 pKa = 10.49VDD243 pKa = 3.21ARR245 pKa = 11.84SNEE248 pKa = 4.06LVFTMKK254 pKa = 10.72DD255 pKa = 3.02GTVPSNALIHH265 pKa = 6.69AAQKK269 pKa = 9.82YY270 pKa = 9.12AAANDD275 pKa = 4.64LIAYY279 pKa = 7.8FSVRR283 pKa = 11.84GANGRR288 pKa = 11.84SAKK291 pKa = 10.25INVSADD297 pKa = 3.26PDD299 pKa = 3.76VNRR302 pKa = 11.84EE303 pKa = 4.04DD304 pKa = 5.23FEE306 pKa = 5.19DD307 pKa = 4.95AINNPGTSNSPTSVNVAVPDD327 pKa = 3.64QVLNDD332 pKa = 3.74ATHH335 pKa = 6.79KK336 pKa = 11.06ANAIANGTYY345 pKa = 10.83AKK347 pKa = 9.36VTTYY351 pKa = 11.37DD352 pKa = 3.44EE353 pKa = 5.64LRR355 pKa = 11.84AAWSNASITYY365 pKa = 9.9IDD367 pKa = 3.61VTAKK371 pKa = 8.72ITYY374 pKa = 10.06SSGAMSARR382 pKa = 11.84ANGASVIVQGNNNIIDD398 pKa = 4.67LGTQTFNWGGITSSTTFTLSNVIAQQGFGGNDD430 pKa = 2.91GNAYY434 pKa = 10.73SLINPSSATQLTANVNNVTLTKK456 pKa = 10.38SDD458 pKa = 3.86SNGNNPIHH466 pKa = 6.85LMYY469 pKa = 10.82GVGSKK474 pKa = 10.62LVFSGTNVFNISNEE488 pKa = 3.54ITRR491 pKa = 11.84SVGSINFANNSSVTLNRR508 pKa = 11.84TSNDD512 pKa = 2.66IAFSQFYY519 pKa = 10.73FEE521 pKa = 4.23TRR523 pKa = 11.84AAAGSVGYY531 pKa = 10.57GNSITMGDD539 pKa = 3.68GSSNTARR546 pKa = 11.84TYY548 pKa = 10.93NGQSANYY555 pKa = 7.72PAVYY559 pKa = 10.92YY560 pKa = 9.67MIDD563 pKa = 3.98KK564 pKa = 9.22ITVGDD569 pKa = 3.79NCNWTQTGFQYY580 pKa = 10.55FINGQQLASSSATYY594 pKa = 10.21TFGQNFNLSIPQSTQPGAIRR614 pKa = 11.84IQGTQKK620 pKa = 10.27VVFNAGTTLDD630 pKa = 3.51INQWASGAVIQMNSSGSSVTFISPKK655 pKa = 8.2QLHH658 pKa = 5.55MQVSTSSGAPNTGPLISGAGTFTMNNSQISTWQGTDD694 pKa = 3.02SQAATPGGNRR704 pKa = 11.84NLKK707 pKa = 9.6FVTMTIRR714 pKa = 11.84NGTTTITDD722 pKa = 3.24VGGNTTSSNIIDD734 pKa = 3.52SSTRR738 pKa = 11.84EE739 pKa = 3.85LATTAIEE746 pKa = 4.39PGTVKK751 pKa = 10.43VNYY754 pKa = 9.15VDD756 pKa = 3.69QYY758 pKa = 10.21GNVVKK763 pKa = 10.01TIDD766 pKa = 3.46VPIDD770 pKa = 3.26KK771 pKa = 10.24DD772 pKa = 3.69VNYY775 pKa = 9.77IGQYY779 pKa = 9.64IDD781 pKa = 5.54LVNKK785 pKa = 10.37DD786 pKa = 3.68YY787 pKa = 11.68ALTNMPANYY796 pKa = 8.24MWAIASQVPPSALTDD811 pKa = 3.65KK812 pKa = 11.17QSGGDD817 pKa = 3.55NTTSADD823 pKa = 3.62DD824 pKa = 3.74GDD826 pKa = 5.15AYY828 pKa = 10.74GQANVAIVPMEE839 pKa = 4.0GTEE842 pKa = 3.93YY843 pKa = 10.16TYY845 pKa = 11.08NIYY848 pKa = 10.82VYY850 pKa = 10.13GQANNSVQYY859 pKa = 10.52QYY861 pKa = 11.88VDD863 pKa = 2.91AKK865 pKa = 9.53TGLVVSSDD873 pKa = 3.8YY874 pKa = 11.27LGSGQEE880 pKa = 3.88ASGTNKK886 pKa = 9.22TPANYY891 pKa = 10.64GNVIDD896 pKa = 4.49WTNSYY901 pKa = 8.14YY902 pKa = 10.96TNDD905 pKa = 3.51LVADD909 pKa = 5.49GYY911 pKa = 11.25HH912 pKa = 6.01YY913 pKa = 10.73ATGSEE918 pKa = 4.41LNGFTQATTTTVGTDD933 pKa = 3.31PTLVTIYY940 pKa = 10.48VAADD944 pKa = 3.42VQTVNVNFVNSDD956 pKa = 3.32GTPLVGQAANVSINGTSGAEE976 pKa = 3.61MSYY979 pKa = 11.72ADD981 pKa = 5.58LISGYY986 pKa = 8.22LTQAGYY992 pKa = 9.47HH993 pKa = 6.33ADD995 pKa = 3.5VNGVFTFDD1003 pKa = 3.38FTANGTSNTDD1013 pKa = 3.34SNPQSLTVTLYY1024 pKa = 10.3PDD1026 pKa = 3.33YY1027 pKa = 11.03QLAGVVGNNQPASDD1041 pKa = 4.22PATGAAYY1048 pKa = 8.33EE1049 pKa = 3.99QLPTSVTEE1057 pKa = 4.03GNYY1060 pKa = 10.11GVAGVSNGAINLGVTDD1076 pKa = 5.15ADD1078 pKa = 4.14LARR1081 pKa = 11.84NGYY1084 pKa = 9.41IYY1086 pKa = 10.44TVTGPDD1092 pKa = 3.3GQTYY1096 pKa = 7.91ATLAEE1101 pKa = 4.35ALAANPNFDD1110 pKa = 3.5TTTNGAGSVDD1120 pKa = 3.58NTPQGFVVNYY1130 pKa = 10.25DD1131 pKa = 3.25VDD1133 pKa = 4.06PVSEE1137 pKa = 4.3STSMSEE1143 pKa = 4.36SEE1145 pKa = 4.43SLSSSVSDD1153 pKa = 3.98SEE1155 pKa = 4.44SDD1157 pKa = 3.58STSTSISDD1165 pKa = 3.86SEE1167 pKa = 4.4SDD1169 pKa = 3.61STSTSISDD1177 pKa = 3.65SEE1179 pKa = 4.42STSRR1183 pKa = 11.84SEE1185 pKa = 5.6DD1186 pKa = 3.36STSDD1190 pKa = 3.85SISSSISEE1198 pKa = 4.38SEE1200 pKa = 4.5SISTSDD1206 pKa = 4.64SISDD1210 pKa = 4.09SISDD1214 pKa = 4.05SEE1216 pKa = 4.77SDD1218 pKa = 3.61SLSNSISDD1226 pKa = 4.29SISDD1230 pKa = 4.05SEE1232 pKa = 4.77SDD1234 pKa = 3.61SLSNSISDD1242 pKa = 4.54SISDD1246 pKa = 3.79STSDD1250 pKa = 4.35SISDD1254 pKa = 4.29SISDD1258 pKa = 4.46SISDD1262 pKa = 3.79STSDD1266 pKa = 4.35SISDD1270 pKa = 4.29SISDD1274 pKa = 4.41SISDD1278 pKa = 3.77SLSNSISDD1286 pKa = 4.54SISDD1290 pKa = 3.79STSDD1294 pKa = 4.35SISDD1298 pKa = 4.29SISDD1302 pKa = 4.28SISSSEE1308 pKa = 4.19SLSEE1312 pKa = 4.63SISEE1316 pKa = 4.19SQSLSISISEE1326 pKa = 4.26SDD1328 pKa = 3.63STSDD1332 pKa = 4.25SISDD1336 pKa = 3.98SEE1338 pKa = 4.52SDD1340 pKa = 4.25SISDD1344 pKa = 4.43SISDD1348 pKa = 4.46SISDD1352 pKa = 3.79STSDD1356 pKa = 4.02SISDD1360 pKa = 3.85SEE1362 pKa = 4.45SLSEE1366 pKa = 4.23SVSEE1370 pKa = 4.46SEE1372 pKa = 4.56SLSISISEE1380 pKa = 4.26SDD1382 pKa = 3.6STSDD1386 pKa = 3.51SLSDD1390 pKa = 3.91SEE1392 pKa = 5.25SDD1394 pKa = 3.94SISNSISDD1402 pKa = 4.81SISDD1406 pKa = 3.67STSNSISDD1414 pKa = 3.92SEE1416 pKa = 4.43SLSEE1420 pKa = 4.23SVSEE1424 pKa = 4.46SEE1426 pKa = 4.56SLSISISEE1434 pKa = 4.26SDD1436 pKa = 3.6STSDD1440 pKa = 3.51SLSDD1444 pKa = 3.91SEE1446 pKa = 4.94SDD1448 pKa = 4.24SISDD1452 pKa = 4.43SISDD1456 pKa = 4.51SISSSISNSDD1466 pKa = 3.5SLSEE1470 pKa = 4.16SVSKK1474 pKa = 11.08SEE1476 pKa = 4.38SLSISISEE1484 pKa = 4.33STSTSTSISDD1494 pKa = 3.58SSSTSTSTSNSDD1506 pKa = 3.24SDD1508 pKa = 4.02SLSNSISDD1516 pKa = 3.81SRR1518 pKa = 11.84SEE1520 pKa = 3.83

Molecular weight: 158.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R2QCP4|R2QCP4_9ENTE Alternate signal-mediated exported protein OS=Enterococcus pallens ATCC BAA-351 OX=1158607 GN=UAU_01942 PE=4 SV=1
RR1 pKa = 7.38IRR3 pKa = 11.84TVIQTRR9 pKa = 11.84TATQIPIATRR19 pKa = 11.84IRR21 pKa = 11.84TVIQILIVTRR31 pKa = 11.84IPIVIQTQTATQTRR45 pKa = 11.84TVIQTRR51 pKa = 11.84IVIQIRR57 pKa = 11.84TAIQIPIVTRR67 pKa = 11.84IPIVIRR73 pKa = 11.84IRR75 pKa = 11.84TVTQIPIATQTPIAIQILIRR95 pKa = 11.84NFRR98 pKa = 11.84QLL100 pKa = 3.1

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5181

0

5181

1566595

30

3111

302.4

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.327 ± 0.041

0.728 ± 0.011

5.251 ± 0.032

7.425 ± 0.044

4.478 ± 0.031

6.515 ± 0.037

1.74 ± 0.017

7.42 ± 0.039

6.583 ± 0.035

9.964 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.019

4.555 ± 0.026

3.592 ± 0.02

4.486 ± 0.03

3.829 ± 0.024

6.143 ± 0.043

5.851 ± 0.038

6.809 ± 0.026

0.943 ± 0.012

3.73 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski