Southern tomato virus (STV)
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8R3Y6|A8R3Y6_STV CP OS=Southern tomato virus OX=591166 GN=p42 PE=4 SV=1
MM1 pKa = 7.5 AGVGGSAAGRR11 pKa = 11.84 VPNAANVPLTAKK23 pKa = 9.91 EE24 pKa = 3.98 KK25 pKa = 9.88 EE26 pKa = 3.99 RR27 pKa = 11.84 TVMRR31 pKa = 11.84 EE32 pKa = 3.24 IVEE35 pKa = 3.91 IGEE38 pKa = 4.43 TFVEE42 pKa = 3.98 LGIDD46 pKa = 2.94 KK47 pKa = 10.55 RR48 pKa = 11.84 YY49 pKa = 8.46 FQRR52 pKa = 11.84 TTYY55 pKa = 9.6 VSHH58 pKa = 6.77 MLLPNQYY65 pKa = 10.32 FKK67 pKa = 11.31 LLKK70 pKa = 9.7 QFKK73 pKa = 10.12 GKK75 pKa = 7.61 TAEE78 pKa = 4.17 EE79 pKa = 4.12 LDD81 pKa = 4.06 LALGAAVAHH90 pKa = 5.78 GVLRR94 pKa = 11.84 SMRR97 pKa = 11.84 GITFKK102 pKa = 11.14 KK103 pKa = 10.6 FFDD106 pKa = 3.58 FLNWVKK112 pKa = 10.07 TKK114 pKa = 10.18 EE115 pKa = 4.3 GKK117 pKa = 9.79 DD118 pKa = 3.35 ALGEE122 pKa = 3.82 TMYY125 pKa = 10.88 AQKK128 pKa = 10.81 LEE130 pKa = 4.11 QKK132 pKa = 10.57 GRR134 pKa = 11.84 GDD136 pKa = 3.38 FSIAEE141 pKa = 4.19 VALLHH146 pKa = 6.82 CFEE149 pKa = 4.35 TQRR152 pKa = 11.84 NDD154 pKa = 3.13 MLRR157 pKa = 11.84 DD158 pKa = 3.57 EE159 pKa = 4.78 KK160 pKa = 11.01 DD161 pKa = 3.3 VRR163 pKa = 11.84 LKK165 pKa = 11.07 AEE167 pKa = 4.16 EE168 pKa = 4.76 EE169 pKa = 4.02 IADD172 pKa = 4.05 LQRR175 pKa = 11.84 KK176 pKa = 7.21 IVKK179 pKa = 9.8 RR180 pKa = 11.84 RR181 pKa = 11.84 EE182 pKa = 3.84 KK183 pKa = 11.06 LEE185 pKa = 4.0 EE186 pKa = 4.04 DD187 pKa = 4.74 LIATKK192 pKa = 10.66 SNYY195 pKa = 9.64 EE196 pKa = 3.74 PVSRR200 pKa = 11.84 YY201 pKa = 9.93 VGLSDD206 pKa = 4.99 YY207 pKa = 10.97 EE208 pKa = 4.53 LNCKK212 pKa = 9.59 CWSLYY217 pKa = 7.9 QQFNPDD223 pKa = 3.64 KK224 pKa = 8.97 VTAGAKK230 pKa = 7.11 PTRR233 pKa = 11.84 KK234 pKa = 8.95 QVKK237 pKa = 8.99 EE238 pKa = 4.02 AFDD241 pKa = 3.62 MYY243 pKa = 11.49 AEE245 pKa = 4.93 FVAKK249 pKa = 9.49 TNRR252 pKa = 11.84 LEE254 pKa = 3.99 FLKK257 pKa = 10.79 HH258 pKa = 5.96 GNVKK262 pKa = 10.75 DD263 pKa = 3.64 EE264 pKa = 4.2 LQAFINAKK272 pKa = 9.57 ILGYY276 pKa = 9.98 EE277 pKa = 3.86 QLGDD281 pKa = 3.62 KK282 pKa = 10.78 GKK284 pKa = 10.55 SRR286 pKa = 11.84 YY287 pKa = 8.97 LRR289 pKa = 11.84 RR290 pKa = 11.84 QLAAMGVEE298 pKa = 4.02 MDD300 pKa = 4.58 HH301 pKa = 6.4 EE302 pKa = 4.66 VEE304 pKa = 4.95 SEE306 pKa = 4.16 DD307 pKa = 3.58 EE308 pKa = 4.09 EE309 pKa = 4.41 EE310 pKa = 4.38 AAEE313 pKa = 4.22 EE314 pKa = 4.06 PAGGEE319 pKa = 4.09 AEE321 pKa = 4.37 EE322 pKa = 5.54 GEE324 pKa = 4.73 GDD326 pKa = 4.61 DD327 pKa = 4.56 PLQATVGDD335 pKa = 4.2 NPDD338 pKa = 3.18 GEE340 pKa = 4.53 ARR342 pKa = 11.84 GEE344 pKa = 3.92 EE345 pKa = 4.13 DD346 pKa = 3.21 TAVQVASPAVTRR358 pKa = 11.84 NRR360 pKa = 11.84 GRR362 pKa = 11.84 GRR364 pKa = 11.84 GRR366 pKa = 11.84 VTPLSQRR373 pKa = 11.84 KK374 pKa = 9.12 DD375 pKa = 3.13 RR376 pKa = 11.84 GG377 pKa = 3.39
Molecular weight: 42.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.525
IPC2_protein 5.601
IPC_protein 5.563
Toseland 5.639
ProMoST 5.741
Dawson 5.614
Bjellqvist 5.703
Wikipedia 5.499
Rodwell 5.55
Grimsley 5.639
Solomon 5.614
Lehninger 5.575
Nozaki 5.766
DTASelect 5.906
Thurlkill 5.715
EMBOSS 5.639
Sillero 5.855
Patrickios 4.571
IPC_peptide 5.626
IPC2_peptide 5.842
IPC2.peptide.svr19 5.974
Protein with the highest isoelectric point:
>tr|A8R3Y6|A8R3Y6_STV CP OS=Southern tomato virus OX=591166 GN=p42 PE=4 SV=1
MM1 pKa = 7.5 AGVGGSAAGRR11 pKa = 11.84 VPNAANVPLTAKK23 pKa = 9.91 EE24 pKa = 3.98 KK25 pKa = 9.88 EE26 pKa = 3.99 RR27 pKa = 11.84 TVMRR31 pKa = 11.84 EE32 pKa = 3.24 IVEE35 pKa = 3.91 IGEE38 pKa = 4.43 TFVEE42 pKa = 3.98 LGIDD46 pKa = 2.94 KK47 pKa = 10.55 RR48 pKa = 11.84 YY49 pKa = 8.46 FQRR52 pKa = 11.84 TTYY55 pKa = 9.6 VSHH58 pKa = 6.77 MLLPNQYY65 pKa = 10.32 FKK67 pKa = 11.31 LLKK70 pKa = 9.7 QFKK73 pKa = 10.12 GKK75 pKa = 7.61 TAEE78 pKa = 4.17 EE79 pKa = 4.12 LDD81 pKa = 4.06 LALGAAVAHH90 pKa = 5.78 GVLRR94 pKa = 11.84 SMRR97 pKa = 11.84 GITFKK102 pKa = 11.14 KK103 pKa = 10.6 FFDD106 pKa = 3.58 FLNWVKK112 pKa = 10.07 TKK114 pKa = 10.18 EE115 pKa = 4.3 GKK117 pKa = 9.79 DD118 pKa = 3.35 ALGEE122 pKa = 3.82 TMYY125 pKa = 10.88 AQKK128 pKa = 10.81 LEE130 pKa = 4.11 QKK132 pKa = 10.57 GRR134 pKa = 11.84 GDD136 pKa = 3.38 FSIAEE141 pKa = 4.19 VALLHH146 pKa = 6.82 CFEE149 pKa = 4.35 TQRR152 pKa = 11.84 NDD154 pKa = 3.13 MLRR157 pKa = 11.84 DD158 pKa = 3.57 EE159 pKa = 4.78 KK160 pKa = 11.01 DD161 pKa = 3.3 VRR163 pKa = 11.84 LKK165 pKa = 11.07 AEE167 pKa = 4.16 EE168 pKa = 4.76 EE169 pKa = 4.02 IADD172 pKa = 4.05 LQRR175 pKa = 11.84 KK176 pKa = 7.21 IVKK179 pKa = 9.8 RR180 pKa = 11.84 RR181 pKa = 11.84 EE182 pKa = 3.84 KK183 pKa = 11.06 LEE185 pKa = 4.0 EE186 pKa = 4.04 DD187 pKa = 4.74 LIATKK192 pKa = 10.66 SNYY195 pKa = 9.64 EE196 pKa = 3.74 PVSRR200 pKa = 11.84 YY201 pKa = 9.93 VGLSDD206 pKa = 4.99 YY207 pKa = 10.97 EE208 pKa = 4.53 LNCKK212 pKa = 9.59 CWSLYY217 pKa = 7.9 QQFNPDD223 pKa = 3.64 KK224 pKa = 8.97 VTAGAKK230 pKa = 7.11 PTRR233 pKa = 11.84 KK234 pKa = 8.95 QVKK237 pKa = 8.99 EE238 pKa = 4.02 AFDD241 pKa = 3.62 MYY243 pKa = 11.49 AEE245 pKa = 4.93 FVAKK249 pKa = 9.49 TNRR252 pKa = 11.84 LEE254 pKa = 3.99 FLKK257 pKa = 10.79 HH258 pKa = 5.96 GNVKK262 pKa = 10.75 DD263 pKa = 3.64 EE264 pKa = 4.2 LQAFINAKK272 pKa = 9.57 ILGYY276 pKa = 9.98 EE277 pKa = 3.79 QLGDD281 pKa = 3.35 KK282 pKa = 10.73 GRR284 pKa = 11.84 VVILGVSWQQWVLRR298 pKa = 11.84 WIMKK302 pKa = 8.44 WNQRR306 pKa = 11.84 TRR308 pKa = 11.84 KK309 pKa = 9.37 RR310 pKa = 11.84 LLKK313 pKa = 9.9 NLPVGKK319 pKa = 9.86 LRR321 pKa = 11.84 RR322 pKa = 11.84 GKK324 pKa = 10.63 EE325 pKa = 3.77 MTPCRR330 pKa = 11.84 PLSEE334 pKa = 5.03 IIPMEE339 pKa = 4.0 RR340 pKa = 11.84 LEE342 pKa = 4.28 EE343 pKa = 4.11 KK344 pKa = 9.77 RR345 pKa = 11.84 TLQYY349 pKa = 11.19 KK350 pKa = 10.0 LPPQLSRR357 pKa = 11.84 ATEE360 pKa = 4.07 VEE362 pKa = 4.03 AEE364 pKa = 4.34 AVSLLYY370 pKa = 10.28 PNARR374 pKa = 11.84 IEE376 pKa = 4.27 VEE378 pKa = 3.92 VRR380 pKa = 11.84 PIGRR384 pKa = 11.84 DD385 pKa = 2.92 TRR387 pKa = 11.84 AIPFSRR393 pKa = 11.84 SRR395 pKa = 11.84 WEE397 pKa = 3.61 VGVRR401 pKa = 11.84 KK402 pKa = 10.16 VIGGGEE408 pKa = 4.16 VLNWDD413 pKa = 3.15 IDD415 pKa = 3.87 GNKK418 pKa = 9.27 YY419 pKa = 10.48 RR420 pKa = 11.84 GGGCFADD427 pKa = 4.98 AIKK430 pKa = 10.72 LLAGATQRR438 pKa = 11.84 PPQRR442 pKa = 11.84 LLEE445 pKa = 4.32 DD446 pKa = 4.67 CYY448 pKa = 10.97 TITTARR454 pKa = 11.84 EE455 pKa = 4.0 ALGLPSDD462 pKa = 4.18 LAVPSGKK469 pKa = 10.35 GSCHH473 pKa = 4.37 VRR475 pKa = 11.84 NYY477 pKa = 11.06 NDD479 pKa = 4.61 DD480 pKa = 3.69 ATSGPFLWSFGIKK493 pKa = 9.58 KK494 pKa = 10.38 KK495 pKa = 11.0 YY496 pKa = 10.64 GLDD499 pKa = 3.39 KK500 pKa = 10.93 LLQSLMEE507 pKa = 5.82 DD508 pKa = 4.08 IYY510 pKa = 11.17 CHH512 pKa = 5.8 YY513 pKa = 10.21 SISEE517 pKa = 4.01 ATDD520 pKa = 2.97 RR521 pKa = 11.84 ALPYY525 pKa = 9.61 FAARR529 pKa = 11.84 VGFRR533 pKa = 11.84 SKK535 pKa = 10.99 LLTMGEE541 pKa = 4.09 AVKK544 pKa = 10.66 KK545 pKa = 8.92 FTEE548 pKa = 4.34 CAPMGRR554 pKa = 11.84 CVMMLDD560 pKa = 3.88 ALEE563 pKa = 4.38 QFASAPLYY571 pKa = 10.68 NVLSKK576 pKa = 9.88 YY577 pKa = 7.18 TAEE580 pKa = 4.2 RR581 pKa = 11.84 SRR583 pKa = 11.84 GQTSFRR589 pKa = 11.84 NTVVRR594 pKa = 11.84 ASSDD598 pKa = 2.58 WMHH601 pKa = 6.37 FWDD604 pKa = 4.48 EE605 pKa = 4.08 VKK607 pKa = 10.56 EE608 pKa = 3.96 AAVCVEE614 pKa = 4.37 LDD616 pKa = 2.87 WSKK619 pKa = 10.97 FDD621 pKa = 4.32 RR622 pKa = 11.84 EE623 pKa = 4.37 RR624 pKa = 11.84 PSEE627 pKa = 4.45 DD628 pKa = 3.81 LDD630 pKa = 4.05 FMIKK634 pKa = 9.94 VICSCFRR641 pKa = 11.84 PKK643 pKa = 11.14 DD644 pKa = 3.65 EE645 pKa = 5.22 VEE647 pKa = 3.98 AKK649 pKa = 10.34 LLRR652 pKa = 11.84 GYY654 pKa = 7.99 GVCMRR659 pKa = 11.84 RR660 pKa = 11.84 ALVEE664 pKa = 3.84 RR665 pKa = 11.84 RR666 pKa = 11.84 LITDD670 pKa = 3.59 DD671 pKa = 3.93 GGVIHH676 pKa = 7.49 IDD678 pKa = 3.22 GMVPSGSLWTGWLDD692 pKa = 3.31 TALNILYY699 pKa = 9.46 IKK701 pKa = 10.52 SVLRR705 pKa = 11.84 SIDD708 pKa = 3.29 ILEE711 pKa = 4.4 EE712 pKa = 3.76 EE713 pKa = 4.97 AVPKK717 pKa = 10.71 CAGDD721 pKa = 3.89 DD722 pKa = 3.7 NLTVFSKK729 pKa = 11.11 DD730 pKa = 3.06 PGDD733 pKa = 3.68 EE734 pKa = 4.03 VLEE737 pKa = 4.11 EE738 pKa = 4.04 MRR740 pKa = 11.84 VKK742 pKa = 11.03 LNDD745 pKa = 3.26 YY746 pKa = 10.05 FRR748 pKa = 11.84 AGIKK752 pKa = 10.26 KK753 pKa = 9.56 EE754 pKa = 3.83 DD755 pKa = 4.49 FIITRR760 pKa = 11.84 PPFHH764 pKa = 6.86 VRR766 pKa = 11.84 TFQAVFKK773 pKa = 10.6 EE774 pKa = 4.42 GTDD777 pKa = 3.7 LSKK780 pKa = 10.65 GTSKK784 pKa = 10.38 IMKK787 pKa = 7.88 KK788 pKa = 10.37 AYY790 pKa = 8.18 WRR792 pKa = 11.84 EE793 pKa = 3.85 FEE795 pKa = 4.55 DD796 pKa = 4.09 EE797 pKa = 4.24 LRR799 pKa = 11.84 IDD801 pKa = 3.6 QEE803 pKa = 4.13 KK804 pKa = 10.52 GLSHH808 pKa = 6.48 RR809 pKa = 11.84 WEE811 pKa = 4.11 YY812 pKa = 10.74 RR813 pKa = 11.84 FKK815 pKa = 10.95 GAPKK819 pKa = 10.11 FLSCYY824 pKa = 7.78 WLEE827 pKa = 4.37 DD828 pKa = 3.27 GRR830 pKa = 11.84 PIRR833 pKa = 11.84 PSHH836 pKa = 6.29 EE837 pKa = 4.05 NLEE840 pKa = 4.13 KK841 pKa = 10.74 LLYY844 pKa = 10.04 PEE846 pKa = 5.31 GVHH849 pKa = 7.09 ANIDD853 pKa = 3.66 DD854 pKa = 4.38 YY855 pKa = 11.46 IAAVLSMVVDD865 pKa = 3.74 NPFNHH870 pKa = 6.89 HH871 pKa = 6.72 NINHH875 pKa = 6.6 LKK877 pKa = 9.79 HH878 pKa = 6.58 RR879 pKa = 11.84 YY880 pKa = 9.04 IIANQVKK887 pKa = 9.55 RR888 pKa = 11.84 LSAAGGRR895 pKa = 11.84 CEE897 pKa = 5.8 DD898 pKa = 3.36 ILRR901 pKa = 11.84 LARR904 pKa = 11.84 IRR906 pKa = 11.84 EE907 pKa = 4.14 RR908 pKa = 11.84 EE909 pKa = 4.14 DD910 pKa = 3.24 EE911 pKa = 4.38 EE912 pKa = 5.41 VPVPQIAVWRR922 pKa = 11.84 RR923 pKa = 11.84 VKK925 pKa = 10.55 EE926 pKa = 3.98 YY927 pKa = 11.27 VDD929 pKa = 3.81 LDD931 pKa = 3.68 SYY933 pKa = 11.59 EE934 pKa = 3.86 PAKK937 pKa = 11.0 YY938 pKa = 10.41 YY939 pKa = 10.34 IQDD942 pKa = 3.43 FNAFVSGVTSLYY954 pKa = 10.76 ARR956 pKa = 11.84 SSTGGLDD963 pKa = 3.03 AYY965 pKa = 10.18 KK966 pKa = 11.08 VMDD969 pKa = 5.6 LIRR972 pKa = 11.84 GNATIGRR979 pKa = 11.84 GQWGNDD985 pKa = 2.89 VMDD988 pKa = 5.0 WIRR991 pKa = 11.84 FVRR994 pKa = 11.84 DD995 pKa = 3.54 HH996 pKa = 7.32 PATKK1000 pKa = 10.02 YY1001 pKa = 10.84 LKK1003 pKa = 8.44 GAKK1006 pKa = 9.55 RR1007 pKa = 11.84 FRR1009 pKa = 11.84 EE1010 pKa = 3.89 QHH1012 pKa = 4.89 NQEE1015 pKa = 3.95 PTTAKK1020 pKa = 9.7 PSKK1023 pKa = 9.65 DD1024 pKa = 2.76 ARR1026 pKa = 11.84 RR1027 pKa = 11.84 AIKK1030 pKa = 10.29 LLRR1033 pKa = 11.84 NSLINEE1039 pKa = 4.76 AYY1041 pKa = 10.27 ADD1043 pKa = 3.95 SNSFAISISEE1053 pKa = 3.86 RR1054 pKa = 11.84 LRR1056 pKa = 11.84 RR1057 pKa = 11.84 KK1058 pKa = 10.13 KK1059 pKa = 9.24 PTRR1062 pKa = 3.52
Molecular weight: 121.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.467
IPC2_protein 8.39
IPC_protein 8.229
Toseland 8.843
ProMoST 8.843
Dawson 9.238
Bjellqvist 9.121
Wikipedia 9.37
Rodwell 9.414
Grimsley 9.224
Solomon 9.268
Lehninger 9.224
Nozaki 9.268
DTASelect 9.004
Thurlkill 9.136
EMBOSS 9.385
Sillero 9.311
Patrickios 4.8
IPC_peptide 9.253
IPC2_peptide 7.775
IPC2.peptide.svr19 7.66
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1439
377
1062
719.5
82.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.131 ± 0.679
1.39 ± 0.284
6.046 ± 0.026
8.756 ± 1.268
4.031 ± 0.025
7.019 ± 0.703
1.598 ± 0.13
4.517 ± 0.892
7.922 ± 0.271
9.034 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.293 ± 0.045
3.475 ± 0.013
3.683 ± 0.366
3.197 ± 0.501
8.2 ± 0.37
4.656 ± 0.705
4.378 ± 0.19
6.741 ± 0.075
1.529 ± 0.478
3.405 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here