Phaeobacter sp. CECT 5382

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Phaeobacter; unclassified Phaeobacter

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3864 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P1GXQ1|A0A0P1GXQ1_9RHOB p-hydroxybenzoate hydroxylase OS=Phaeobacter sp. CECT 5382 OX=1712645 GN=pobA PE=4 SV=1
MM1 pKa = 7.15ATATEE6 pKa = 4.17LDD8 pKa = 3.69INTSATATQMANEE21 pKa = 4.13IFGDD25 pKa = 3.75GTTVVSASYY34 pKa = 9.81TGDD37 pKa = 3.28VDD39 pKa = 4.2SSGIYY44 pKa = 9.35TGADD48 pKa = 3.03TTVAGVAPADD58 pKa = 3.49NGVILSTGDD67 pKa = 3.39VVDD70 pKa = 4.49FTNNSGTTNTNTSGNTSTNTSGVNGDD96 pKa = 3.45SDD98 pKa = 4.12FNAIAGSATYY108 pKa = 10.31DD109 pKa = 3.33AAFLEE114 pKa = 4.74IDD116 pKa = 5.04FIPDD120 pKa = 3.32GDD122 pKa = 4.26VLTLDD127 pKa = 4.47FVLSSEE133 pKa = 4.73EE134 pKa = 4.0YY135 pKa = 10.18PEE137 pKa = 4.54FASSQYY143 pKa = 10.97NDD145 pKa = 3.52VVGVWVNGVQAQVTIGDD162 pKa = 4.2GSASIGNINQSDD174 pKa = 3.9TQNIYY179 pKa = 10.83VDD181 pKa = 3.6NTSDD185 pKa = 3.72QFNTEE190 pKa = 3.6MDD192 pKa = 3.68GFTITLTFTAPVNAGAVNSIKK213 pKa = 11.0VGVADD218 pKa = 4.49VADD221 pKa = 3.75SSYY224 pKa = 10.74DD225 pKa = 3.65TNLLIAGGSVQSAFVAQDD243 pKa = 3.6DD244 pKa = 4.62SLTMPLAATRR254 pKa = 11.84MLDD257 pKa = 4.11VLDD260 pKa = 4.67NDD262 pKa = 3.89AATAGGTLTITHH274 pKa = 6.72INDD277 pKa = 3.24VAVTAGDD284 pKa = 3.96TVTLSSGQQITLNADD299 pKa = 3.09GTLEE303 pKa = 4.11VVADD307 pKa = 4.19GDD309 pKa = 4.14AEE311 pKa = 4.35TVYY314 pKa = 10.79FNYY317 pKa = 9.88TVEE320 pKa = 4.15NDD322 pKa = 4.01LGDD325 pKa = 3.75SDD327 pKa = 4.13TALVEE332 pKa = 3.91ITQAQPCFLRR342 pKa = 11.84GTLIVTEE349 pKa = 5.04DD350 pKa = 3.84GPLPIEE356 pKa = 4.4DD357 pKa = 5.06LRR359 pKa = 11.84PGMLVEE365 pKa = 4.75TYY367 pKa = 11.23DD368 pKa = 4.7HH369 pKa = 7.06GLQPLRR375 pKa = 11.84WIGHH379 pKa = 4.31SHH381 pKa = 4.35VTVSEE386 pKa = 4.15TTAPIRR392 pKa = 11.84FKK394 pKa = 10.62KK395 pKa = 9.1GQYY398 pKa = 10.11GCEE401 pKa = 3.68QDD403 pKa = 4.52LWVSPNHH410 pKa = 6.13RR411 pKa = 11.84MLVTGQFAEE420 pKa = 4.27LLFGEE425 pKa = 4.65SEE427 pKa = 4.46VLAKK431 pKa = 10.8AKK433 pKa = 10.7DD434 pKa = 3.77LVNDD438 pKa = 3.5SSIRR442 pKa = 11.84PDD444 pKa = 3.08HH445 pKa = 6.52SFEE448 pKa = 4.09TVEE451 pKa = 4.0YY452 pKa = 9.97FHH454 pKa = 7.97LLFDD458 pKa = 3.32QHH460 pKa = 6.9EE461 pKa = 4.51ILWSNGALSEE471 pKa = 4.51SYY473 pKa = 10.73HH474 pKa = 6.54PGPEE478 pKa = 3.89TTRR481 pKa = 11.84GFDD484 pKa = 3.56AEE486 pKa = 4.39TQAEE490 pKa = 4.47LFALFPEE497 pKa = 5.14LCPMTGTGYY506 pKa = 10.59GAAARR511 pKa = 11.84LSLRR515 pKa = 11.84HH516 pKa = 6.02FEE518 pKa = 4.27TVLLDD523 pKa = 3.63TYY525 pKa = 10.88MSS527 pKa = 3.64

Molecular weight: 55.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1H4K5|A0A0P1H4K5_9RHOB Uncharacterized protein OS=Phaeobacter sp. CECT 5382 OX=1712645 GN=PH5382_03865 PE=4 SV=1
MM1 pKa = 6.84TACPTFAGPALFSYY15 pKa = 9.41GFRR18 pKa = 11.84PFFAAACIFGFLVVPLWVLIWRR40 pKa = 11.84GSLEE44 pKa = 4.22YY45 pKa = 9.96QGHH48 pKa = 6.36FGAVDD53 pKa = 3.07WHH55 pKa = 6.08IHH57 pKa = 4.59EE58 pKa = 5.21MIFGYY63 pKa = 10.66GSAVLAGFLFTAVPNWTARR82 pKa = 11.84MPVRR86 pKa = 11.84GLPLAVLFAVWILGRR101 pKa = 11.84LALWGGLVAPGLALVVEE118 pKa = 5.07CSFLLLVAAMIARR131 pKa = 11.84EE132 pKa = 4.06IIAGKK137 pKa = 8.27NWRR140 pKa = 11.84NLKK143 pKa = 10.33VLVPVLLLGAGNILFHH159 pKa = 8.03LEE161 pKa = 4.23VILSGASDD169 pKa = 3.36YY170 pKa = 11.34GRR172 pKa = 11.84RR173 pKa = 11.84FGIALLVFLIMLIGGRR189 pKa = 11.84IIPSFTRR196 pKa = 11.84NWLAKK201 pKa = 10.08EE202 pKa = 4.07RR203 pKa = 11.84GQDD206 pKa = 3.42GPMPIPFSRR215 pKa = 11.84FDD217 pKa = 3.57AVALAFGVAALVGWTLAPEE236 pKa = 4.44SVVSAVLLLLAGVLHH251 pKa = 7.33IARR254 pKa = 11.84LTRR257 pKa = 11.84WQGQAVLRR265 pKa = 11.84SPLLVMLHH273 pKa = 5.56VAYY276 pKa = 9.83IFIPLGLMVSGFAALGLVEE295 pKa = 4.49QVAAVHH301 pKa = 6.17LLGIGAIGGMTVAVMMRR318 pKa = 11.84ATLGHH323 pKa = 6.2TGRR326 pKa = 11.84ALVAGPVLTLGFGLLLAAAVARR348 pKa = 11.84IGSGILADD356 pKa = 4.4LGLDD360 pKa = 3.68GVLLSAGFWTVSFAVLCLRR379 pKa = 11.84MLPWLVLPKK388 pKa = 10.22AARR391 pKa = 11.84RR392 pKa = 11.84KK393 pKa = 9.47PNPAPQQAVNPSPKK407 pKa = 9.93VAGG410 pKa = 3.68

Molecular weight: 43.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3864

0

3864

1199402

30

2393

310.4

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.911 ± 0.055

0.975 ± 0.013

5.848 ± 0.032

5.946 ± 0.038

3.765 ± 0.027

8.44 ± 0.041

2.115 ± 0.02

5.106 ± 0.028

3.523 ± 0.032

10.321 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.82 ± 0.018

2.745 ± 0.02

4.902 ± 0.029

3.802 ± 0.025

6.23 ± 0.037

5.628 ± 0.034

5.356 ± 0.031

6.94 ± 0.031

1.375 ± 0.017

2.254 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski