Shuangao Insect Virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Peribunyaviridae; Shangavirus; Insect shangavirus

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0B5KS64|A0A0B5KS64_9VIRU Nucleocapsid protein OS=Shuangao Insect Virus 1 OX=1608075 GN=N PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.41RR3 pKa = 11.84FILIINILLTAYY15 pKa = 9.73ARR17 pKa = 11.84PNNCGNGAVMVCEE30 pKa = 4.25KK31 pKa = 9.81TYY33 pKa = 11.27SPGLSMCLRR42 pKa = 11.84DD43 pKa = 4.29DD44 pKa = 5.16LGSQSVWSDD53 pKa = 3.02EE54 pKa = 4.13KK55 pKa = 11.52GVINYY60 pKa = 9.1LEE62 pKa = 4.24RR63 pKa = 11.84TLDD66 pKa = 3.38TGVCDD71 pKa = 4.78LVEE74 pKa = 4.64AEE76 pKa = 4.44NGYY79 pKa = 10.84LNYY82 pKa = 10.38HH83 pKa = 6.92LYY85 pKa = 10.0EE86 pKa = 4.86CNAGTQTKK94 pKa = 8.4TFVCSKK100 pKa = 9.04SCNVEE105 pKa = 3.45TDD107 pKa = 3.05KK108 pKa = 11.49LNKK111 pKa = 9.35VLHH114 pKa = 6.59LSTHH118 pKa = 4.17GRR120 pKa = 11.84NYY122 pKa = 10.87YY123 pKa = 10.22VISINNDD130 pKa = 2.4ISSGWFEE137 pKa = 4.08NRR139 pKa = 11.84KK140 pKa = 8.79MIPIEE145 pKa = 3.83QPKK148 pKa = 10.24NKK150 pKa = 9.58IHH152 pKa = 5.77ITCGKK157 pKa = 9.76NSQQFVIDD165 pKa = 3.84YY166 pKa = 9.69KK167 pKa = 11.44LHH169 pKa = 6.75DD170 pKa = 4.06EE171 pKa = 4.61CEE173 pKa = 4.15IALGDD178 pKa = 3.76YY179 pKa = 10.36IPRR182 pKa = 11.84LFLRR186 pKa = 11.84PLCSNASLILSLIFFIICMFIVILLTQYY214 pKa = 10.31SQVRR218 pKa = 11.84ALIGTLFFIPVYY230 pKa = 10.51LFEE233 pKa = 4.32KK234 pKa = 10.65LKK236 pKa = 11.21LKK238 pKa = 10.89VCGTCKK244 pKa = 10.43SCGQTVHH251 pKa = 6.06YY252 pKa = 9.57FRR254 pKa = 11.84RR255 pKa = 11.84CKK257 pKa = 8.87KK258 pKa = 8.66TCGCGYY264 pKa = 9.56IGNSIDD270 pKa = 3.37EE271 pKa = 4.15LRR273 pKa = 11.84IHH275 pKa = 7.03KK276 pKa = 9.32EE277 pKa = 3.77RR278 pKa = 11.84HH279 pKa = 5.42CSGDD283 pKa = 3.2SRR285 pKa = 11.84LLIATEE291 pKa = 4.05YY292 pKa = 11.14ASTRR296 pKa = 11.84FLKK299 pKa = 9.98TVIAVLLTLVLLSLITPISGEE320 pKa = 3.81TRR322 pKa = 11.84EE323 pKa = 4.01QEE325 pKa = 3.91RR326 pKa = 11.84EE327 pKa = 4.19IMCKK331 pKa = 7.85TWGICNNLVTEE342 pKa = 4.42SPLIKK347 pKa = 10.62LLGGTRR353 pKa = 11.84KK354 pKa = 9.7NLSDD358 pKa = 3.53YY359 pKa = 10.1YY360 pKa = 9.59YY361 pKa = 11.19AVVTEE366 pKa = 4.54EE367 pKa = 4.17STEE370 pKa = 4.15VTTTTSEE377 pKa = 4.11TPALVEE383 pKa = 3.78IEE385 pKa = 4.38VFDD388 pKa = 4.74FSTLRR393 pKa = 11.84KK394 pKa = 8.96PVILFVEE401 pKa = 4.27LLIVMLCILMLNKK414 pKa = 9.46QVKK417 pKa = 9.17IYY419 pKa = 10.41CASTMLKK426 pKa = 10.33CFLIDD431 pKa = 3.29LDD433 pKa = 4.89KK434 pKa = 10.98RR435 pKa = 11.84LKK437 pKa = 9.42SLKK440 pKa = 10.19YY441 pKa = 10.1NEE443 pKa = 4.37FSVKK447 pKa = 9.32EE448 pKa = 4.06VYY450 pKa = 10.46NRR452 pKa = 11.84TTYY455 pKa = 10.62QVTSKK460 pKa = 10.21FLKK463 pKa = 10.56EE464 pKa = 4.06IFSKK468 pKa = 9.83TKK470 pKa = 9.56NRR472 pKa = 11.84NSIDD476 pKa = 2.96ISLCKK481 pKa = 9.82EE482 pKa = 3.68CMMVHH487 pKa = 5.42TKK489 pKa = 10.3KK490 pKa = 10.03PQEE493 pKa = 4.94HH494 pKa = 6.01GTCDD498 pKa = 3.94CGWVDD503 pKa = 4.79EE504 pKa = 5.78DD505 pKa = 6.25YY506 pKa = 11.14IDD508 pKa = 5.56PDD510 pKa = 3.58NLHH513 pKa = 6.2QLTVACLLNVSSLSHH528 pKa = 6.11TLHH531 pKa = 6.87FSNMMRR537 pKa = 11.84KK538 pKa = 9.26VKK540 pKa = 10.7VILFSLIFFLLIGTATAHH558 pKa = 6.84CDD560 pKa = 3.28HH561 pKa = 7.36HH562 pKa = 7.65DD563 pKa = 3.51SGYY566 pKa = 10.8VSDD569 pKa = 5.37VVAAYY574 pKa = 7.23QQCILDD580 pKa = 4.12YY581 pKa = 10.49NGHH584 pKa = 7.41DD585 pKa = 3.26IPKK588 pKa = 10.04CVLSLKK594 pKa = 10.84LEE596 pKa = 4.74DD597 pKa = 3.94PNAMKK602 pKa = 10.44RR603 pKa = 11.84CIDD606 pKa = 3.83EE607 pKa = 3.98PQVRR611 pKa = 11.84YY612 pKa = 7.59TICKK616 pKa = 9.98LMEE619 pKa = 3.78TRR621 pKa = 11.84IMGEE625 pKa = 4.37CISNIAVLANEE636 pKa = 4.31RR637 pKa = 11.84NIIDD641 pKa = 4.01AYY643 pKa = 10.73RR644 pKa = 11.84RR645 pKa = 11.84NKK647 pKa = 9.48TYY649 pKa = 11.36ANMYY653 pKa = 10.2LDD655 pKa = 4.22EE656 pKa = 5.07LKK658 pKa = 9.88STIKK662 pKa = 10.88NFFNDD667 pKa = 3.18PWSLLNKK674 pKa = 9.33MNRR677 pKa = 11.84LTNIMNIYY685 pKa = 9.91AGRR688 pKa = 11.84AVVFVINEE696 pKa = 3.84MNEE699 pKa = 4.3YY700 pKa = 10.8KK701 pKa = 10.52SVTCNGIPPDD711 pKa = 3.78TVIRR715 pKa = 11.84SILLDD720 pKa = 3.7TIEE723 pKa = 3.85IFKK726 pKa = 10.78RR727 pKa = 11.84LEE729 pKa = 3.82APMDD733 pKa = 3.78SEE735 pKa = 5.38QFLGWRR741 pKa = 11.84GKK743 pKa = 9.75QLLEE747 pKa = 3.83TPMTIEE753 pKa = 3.62EE754 pKa = 4.13TTIQKK759 pKa = 10.24RR760 pKa = 11.84EE761 pKa = 3.89VPIGSSTSTEE771 pKa = 4.3LPPTDD776 pKa = 3.71ATLSSSDD783 pKa = 3.5EE784 pKa = 4.54DD785 pKa = 3.18IMYY788 pKa = 8.15NTGRR792 pKa = 11.84RR793 pKa = 11.84LLTVEE798 pKa = 4.24EE799 pKa = 4.62SEE801 pKa = 4.46DD802 pKa = 3.41TGVKK806 pKa = 9.46PKK808 pKa = 10.48YY809 pKa = 9.69IQGALTTIYY818 pKa = 10.67SCLNTSEE825 pKa = 4.58QYY827 pKa = 10.79ISVGLSNEE835 pKa = 4.11SYY837 pKa = 10.58KK838 pKa = 10.43ISNKK842 pKa = 10.21SLTGLHH848 pKa = 6.04YY849 pKa = 10.61FLDD852 pKa = 3.8ISTDD856 pKa = 3.25TLSYY860 pKa = 10.09YY861 pKa = 10.29INSTTVEE868 pKa = 4.02KK869 pKa = 10.95VITGNISVPKK879 pKa = 10.08IPCHH883 pKa = 3.94YY884 pKa = 9.27TKK886 pKa = 11.11YY887 pKa = 10.72NLDD890 pKa = 3.8SIIAFGPEE898 pKa = 3.56NCPKK902 pKa = 10.98NFMSYY907 pKa = 11.56ANDD910 pKa = 3.66KK911 pKa = 9.97EE912 pKa = 4.39KK913 pKa = 10.9KK914 pKa = 9.44KK915 pKa = 10.41QVLMMSSINSEE926 pKa = 4.01LLVYY930 pKa = 10.06CWAYY934 pKa = 9.81TKK936 pKa = 11.11DD937 pKa = 3.24NTKK940 pKa = 10.31VYY942 pKa = 10.52GYY944 pKa = 9.13IQQGCYY950 pKa = 9.65IYY952 pKa = 10.8LDD954 pKa = 3.5TSEE957 pKa = 5.37GVVHH961 pKa = 7.36DD962 pKa = 4.47FQRR965 pKa = 11.84CYY967 pKa = 11.35DD968 pKa = 3.76WTPLPNDD975 pKa = 3.07KK976 pKa = 10.54FATRR980 pKa = 11.84EE981 pKa = 3.99IIKK984 pKa = 10.35DD985 pKa = 3.24KK986 pKa = 10.76HH987 pKa = 5.81LKK989 pKa = 8.26KK990 pKa = 9.39TIDD993 pKa = 3.38EE994 pKa = 4.41MSWRR998 pKa = 11.84QYY1000 pKa = 8.45VDD1002 pKa = 3.7KK1003 pKa = 10.3YY1004 pKa = 11.15LEE1006 pKa = 4.84PIEE1009 pKa = 5.54VDD1011 pKa = 3.15IQHH1014 pKa = 6.47GKK1016 pKa = 10.32AKK1018 pKa = 9.99VVNAIKK1024 pKa = 10.37SVYY1027 pKa = 9.6KK1028 pKa = 10.53AEE1030 pKa = 3.94KK1031 pKa = 9.05TITIKK1036 pKa = 11.15GITNSKK1042 pKa = 9.07GVEE1045 pKa = 4.11GASVVFDD1052 pKa = 4.77LNLDD1056 pKa = 3.39QYY1058 pKa = 11.34EE1059 pKa = 4.48SVKK1062 pKa = 9.91YY1063 pKa = 8.69TVIDD1067 pKa = 3.37KK1068 pKa = 10.89LGRR1071 pKa = 11.84EE1072 pKa = 4.3LFDD1075 pKa = 3.39ISLSVEE1081 pKa = 4.09SARR1084 pKa = 11.84LTNEE1088 pKa = 3.57YY1089 pKa = 9.32VHH1091 pKa = 6.71SYY1093 pKa = 9.86KK1094 pKa = 10.92APLSKK1099 pKa = 11.01GIDD1102 pKa = 3.33VRR1104 pKa = 11.84STSNCTGACGSFKK1117 pKa = 10.35PRR1119 pKa = 11.84YY1120 pKa = 9.19NSIGYY1125 pKa = 9.68SFDD1128 pKa = 3.36KK1129 pKa = 10.36TSSWGCEE1136 pKa = 3.96EE1137 pKa = 4.34FSCWAIGTGCTWGDD1151 pKa = 3.55CKK1153 pKa = 10.83EE1154 pKa = 4.02IEE1156 pKa = 4.16DD1157 pKa = 4.39RR1158 pKa = 11.84KK1159 pKa = 11.01NGFDD1163 pKa = 2.94IYY1165 pKa = 10.86KK1166 pKa = 10.11RR1167 pKa = 11.84SSDD1170 pKa = 3.64TDD1172 pKa = 3.6LKK1174 pKa = 10.77IATVCVYY1181 pKa = 10.52LLKK1184 pKa = 10.83KK1185 pKa = 9.58GFCVDD1190 pKa = 3.51LTKK1193 pKa = 10.72NDD1195 pKa = 3.46KK1196 pKa = 10.81KK1197 pKa = 11.35VVGEE1201 pKa = 4.59FDD1203 pKa = 4.11FQSKK1207 pKa = 8.45MADD1210 pKa = 3.28TSILPDD1216 pKa = 5.48LITLDD1221 pKa = 3.11SRR1223 pKa = 11.84EE1224 pKa = 4.14TVRR1227 pKa = 11.84IGQINQRR1234 pKa = 11.84GEE1236 pKa = 3.94YY1237 pKa = 10.88GLDD1240 pKa = 3.16CGNYY1244 pKa = 8.37QVVNEE1249 pKa = 4.3TVYY1252 pKa = 10.96GIPTPDD1258 pKa = 2.91IHH1260 pKa = 6.79IHH1262 pKa = 5.32CHH1264 pKa = 5.82GISPKK1269 pKa = 9.4TVSAKK1274 pKa = 9.5TCWKK1278 pKa = 10.35NDD1280 pKa = 3.09NNLCGNLEE1288 pKa = 3.92EE1289 pKa = 4.46MDD1291 pKa = 3.1RR1292 pKa = 11.84SRR1294 pKa = 11.84FNIRR1298 pKa = 11.84RR1299 pKa = 11.84MDD1301 pKa = 3.84NNKK1304 pKa = 8.86MEE1306 pKa = 4.63ISRR1309 pKa = 11.84EE1310 pKa = 3.84HH1311 pKa = 5.27TQIGTAQIAFDD1322 pKa = 3.65IGSLTVEE1329 pKa = 4.33SKK1331 pKa = 10.73EE1332 pKa = 4.35IAGQTHH1338 pKa = 6.46FRR1340 pKa = 11.84PGHH1343 pKa = 5.7CAGCYY1348 pKa = 9.66DD1349 pKa = 3.97CNLGVFCDD1357 pKa = 3.96VNIEE1361 pKa = 4.13TTVRR1365 pKa = 11.84GTCKK1369 pKa = 10.45VNCTCPTTHH1378 pKa = 7.55DD1379 pKa = 3.81GLIVSPEE1386 pKa = 3.84DD1387 pKa = 3.67SLVHH1391 pKa = 6.51LHH1393 pKa = 6.08LTCGAKK1399 pKa = 10.21LRR1401 pKa = 11.84TIRR1404 pKa = 11.84CTICGQQVEE1413 pKa = 5.2FPSEE1417 pKa = 3.79IKK1419 pKa = 9.96EE1420 pKa = 4.21DD1421 pKa = 3.72KK1422 pKa = 10.87SILSVNTPHH1431 pKa = 6.71EE1432 pKa = 4.17RR1433 pKa = 11.84TEE1435 pKa = 4.28IEE1437 pKa = 4.21SFDD1440 pKa = 3.76NKK1442 pKa = 10.2YY1443 pKa = 10.74SSWWTEE1449 pKa = 3.71VIRR1452 pKa = 11.84EE1453 pKa = 3.92IGGFFSGFNILGYY1466 pKa = 10.36FSTVKK1471 pKa = 10.31IIFYY1475 pKa = 9.74VIIGFFLIYY1484 pKa = 10.06LLSIFVVPILKK1495 pKa = 8.42ITYY1498 pKa = 8.88RR1499 pKa = 11.84AIHH1502 pKa = 5.44GTYY1505 pKa = 10.27KK1506 pKa = 10.8NVIKK1510 pKa = 10.63VSKK1513 pKa = 9.47RR1514 pKa = 11.84TQQLEE1519 pKa = 4.15LQEE1522 pKa = 4.3RR1523 pKa = 11.84LAPVKK1528 pKa = 9.57TKK1530 pKa = 10.79RR1531 pKa = 11.84LIKK1534 pKa = 10.34VGRR1537 pKa = 11.84NDD1539 pKa = 3.04

Molecular weight:
174.94 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0B5KS64|A0A0B5KS64_9VIRU Nucleocapsid protein OS=Shuangao Insect Virus 1 OX=1608075 GN=N PE=4 SV=1
MM1 pKa = 7.62SSTKK5 pKa = 10.69AFVEE9 pKa = 4.37RR10 pKa = 11.84NVNAAEE16 pKa = 3.85QAFEE20 pKa = 4.01IDD22 pKa = 4.05RR23 pKa = 11.84PIAVNDD29 pKa = 3.65WSPKK33 pKa = 9.22FGYY36 pKa = 8.3KK37 pKa = 9.07TFEE40 pKa = 3.88EE41 pKa = 5.67YY42 pKa = 10.27YY43 pKa = 10.94GKK45 pKa = 10.01ILGHH49 pKa = 5.98EE50 pKa = 4.21EE51 pKa = 3.57CSTYY55 pKa = 10.71FKK57 pKa = 10.68IADD60 pKa = 4.29KK61 pKa = 10.77IRR63 pKa = 11.84AHH65 pKa = 4.97TTSNKK70 pKa = 9.9GKK72 pKa = 8.08TIRR75 pKa = 11.84YY76 pKa = 8.48RR77 pKa = 11.84LGEE80 pKa = 4.1NLHH83 pKa = 6.26VPIFHH88 pKa = 7.7PSGKK92 pKa = 9.11EE93 pKa = 3.74DD94 pKa = 4.46KK95 pKa = 10.17IDD97 pKa = 3.85AGALTARR104 pKa = 11.84RR105 pKa = 11.84FGGYY109 pKa = 7.28MAKK112 pKa = 10.17LVYY115 pKa = 10.21EE116 pKa = 5.18RR117 pKa = 11.84IQAGDD122 pKa = 3.59KK123 pKa = 10.85DD124 pKa = 4.19YY125 pKa = 11.32LVCINKK131 pKa = 9.34IAAKK135 pKa = 10.34AGIEE139 pKa = 4.05KK140 pKa = 10.21PEE142 pKa = 4.2KK143 pKa = 10.2AGEE146 pKa = 3.77AAKK149 pKa = 10.1IYY151 pKa = 9.97WSLAPGAEE159 pKa = 3.8SHH161 pKa = 6.37YY162 pKa = 11.05DD163 pKa = 3.41SFGLYY168 pKa = 9.52PYY170 pKa = 10.35AHH172 pKa = 6.87EE173 pKa = 4.85AIKK176 pKa = 10.37RR177 pKa = 11.84IQPSRR182 pKa = 11.84YY183 pKa = 8.52NMKK186 pKa = 10.55SKK188 pKa = 10.23VDD190 pKa = 3.65NYY192 pKa = 10.54IGLHH196 pKa = 4.99KK197 pKa = 9.73AARR200 pKa = 11.84MRR202 pKa = 11.84CVDD205 pKa = 3.52NVGASASGLITINEE219 pKa = 4.08FLDD222 pKa = 3.82TEE224 pKa = 4.19EE225 pKa = 5.45GIAALKK231 pKa = 10.61VCVDD235 pKa = 4.37QINKK239 pKa = 9.91ALGKK243 pKa = 10.21SNVGEE248 pKa = 4.17GSTRR252 pKa = 11.84AVFAKK257 pKa = 10.6LQGALKK263 pKa = 10.68NILEE267 pKa = 4.36VNEE270 pKa = 4.08

Molecular weight:
29.94 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

4144

270

2335

1381.3

157.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.344 ± 1.868

2.558 ± 0.806

5.478 ± 0.291

6.733 ± 0.331

4.054 ± 0.225

5.14 ± 0.821

2.365 ± 0.09

7.625 ± 0.588

8.277 ± 0.541

9.097 ± 0.816

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.397

5.767 ± 0.245

3.041 ± 0.069

2.968 ± 0.38

4.247 ± 0.142

7.987 ± 0.752

6.612 ± 0.843

5.864 ± 0.407

0.965 ± 0.056

4.392 ± 0.256

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski