Pectobacterium phage Peat1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Phimunavirus; Pectobacterium virus Peat1

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3YI42|A0A0H3YI42_9CAUD Type VII integral membrane protein OS=Pectobacterium phage Peat1 OX=1654601 PE=4 SV=1
MM1 pKa = 7.42YY2 pKa = 9.95SVQIAVSDD10 pKa = 3.84GTLTRR15 pKa = 11.84IALSIEE21 pKa = 4.16YY22 pKa = 10.07FEE24 pKa = 6.26KK25 pKa = 10.91DD26 pKa = 4.32DD27 pKa = 3.3ITLYY31 pKa = 10.93RR32 pKa = 11.84NLEE35 pKa = 4.1LTPLVLGTDD44 pKa = 3.81WQWDD48 pKa = 3.51GDD50 pKa = 3.9THH52 pKa = 7.88INLLTGIPVPVGSYY66 pKa = 8.03ITVRR70 pKa = 11.84RR71 pKa = 11.84NTDD74 pKa = 2.11IDD76 pKa = 3.69RR77 pKa = 11.84AFNIYY82 pKa = 10.62DD83 pKa = 3.47GGAAFNRR90 pKa = 11.84EE91 pKa = 3.99TLDD94 pKa = 3.47EE95 pKa = 4.33NFKK98 pKa = 11.24QMIYY102 pKa = 10.2LAQEE106 pKa = 3.97FTEE109 pKa = 4.83GNGLTGLYY117 pKa = 10.06FPLDD121 pKa = 3.21MHH123 pKa = 6.89GFQIKK128 pKa = 10.46NLGEE132 pKa = 4.05PTDD135 pKa = 4.43PGDD138 pKa = 4.01AVTKK142 pKa = 10.37QYY144 pKa = 11.57VDD146 pKa = 3.51TANTAQNANFNASQQAQDD164 pKa = 3.44QAVAASQAVQDD175 pKa = 3.98NRR177 pKa = 11.84LASLEE182 pKa = 4.13NTFVQATSSYY192 pKa = 8.88PWYY195 pKa = 9.16TVSTSTTDD203 pKa = 2.82TFTPGFNFTKK213 pKa = 10.29AAVYY217 pKa = 10.47INGVCQTPDD226 pKa = 2.85YY227 pKa = 11.04SYY229 pKa = 11.21IVVANQILLADD240 pKa = 4.48PVPLGTMVFARR251 pKa = 11.84LGEE254 pKa = 4.81DD255 pKa = 3.1IQNDD259 pKa = 3.52DD260 pKa = 4.86DD261 pKa = 4.73FATTAQLSAVQANLQDD277 pKa = 4.64EE278 pKa = 4.5IDD280 pKa = 3.8VTNTEE285 pKa = 4.19VSNKK289 pKa = 9.57ASKK292 pKa = 10.13GANSDD297 pKa = 3.1ISSLSGLTTPLSAAQGGTGNNTGNAATATVLATHH331 pKa = 6.42RR332 pKa = 11.84TIQTNLASTSAASFNGSANITPGVTGNLPVTNGGTGAGSAPSARR376 pKa = 11.84ANLGAAMNGINNDD389 pKa = 3.02ISNLAALTGGITGLTTGTAAASGIVGEE416 pKa = 4.5VASAASSSATNLVSGSVINIISLSLPAGDD445 pKa = 4.53WEE447 pKa = 4.82LEE449 pKa = 4.2SAFQIINTGNVTALAFGVSTTTGVLPTPWYY479 pKa = 9.9DD480 pKa = 3.28VYY482 pKa = 11.5SITTTIGSGTSNRR495 pKa = 11.84QGMARR500 pKa = 11.84RR501 pKa = 11.84VLLSTTTTVYY511 pKa = 10.73LVAQATFTGTATANGYY527 pKa = 8.94VRR529 pKa = 11.84ARR531 pKa = 11.84RR532 pKa = 11.84VRR534 pKa = 3.3

Molecular weight: 55.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3YI90|A0A0H3YI90_9CAUD Uncharacterized protein OS=Pectobacterium phage Peat1 OX=1654601 PE=4 SV=1
MM1 pKa = 7.63SDD3 pKa = 2.58WYY5 pKa = 10.54YY6 pKa = 11.2DD7 pKa = 3.51KK8 pKa = 11.26EE9 pKa = 4.56SGVVYY14 pKa = 10.67RR15 pKa = 11.84NGRR18 pKa = 11.84PMTLLGKK25 pKa = 10.04DD26 pKa = 3.42GYY28 pKa = 11.79VRR30 pKa = 11.84MRR32 pKa = 11.84YY33 pKa = 9.19KK34 pKa = 10.81GRR36 pKa = 11.84VEE38 pKa = 3.8RR39 pKa = 11.84AHH41 pKa = 6.28RR42 pKa = 11.84LAYY45 pKa = 9.47ILQGLPLPKK54 pKa = 10.04QVDD57 pKa = 4.17HH58 pKa = 7.16INGNRR63 pKa = 11.84ADD65 pKa = 3.9NRR67 pKa = 11.84WVNLRR72 pKa = 11.84PATNTQNQYY81 pKa = 9.2NRR83 pKa = 11.84RR84 pKa = 11.84PASKK88 pKa = 10.26LGHH91 pKa = 6.28KK92 pKa = 9.88KK93 pKa = 9.55GAYY96 pKa = 8.25MNIGGRR102 pKa = 11.84TWYY105 pKa = 10.78SLLRR109 pKa = 11.84YY110 pKa = 8.55NGKK113 pKa = 10.12RR114 pKa = 11.84IYY116 pKa = 10.75LGTFKK121 pKa = 10.75TEE123 pKa = 4.3DD124 pKa = 3.78EE125 pKa = 3.93AHH127 pKa = 6.27AAYY130 pKa = 9.37TEE132 pKa = 4.11ASKK135 pKa = 10.61KK136 pKa = 7.97YY137 pKa = 9.36HH138 pKa = 6.29GNFSRR143 pKa = 11.84TEE145 pKa = 3.65

Molecular weight: 16.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

14173

40

939

232.3

25.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.829 ± 0.48

1.192 ± 0.187

5.969 ± 0.206

5.172 ± 0.33

2.921 ± 0.143

7.571 ± 0.268

1.849 ± 0.2

4.572 ± 0.22

4.502 ± 0.249

8.707 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.186

4.389 ± 0.299

3.965 ± 0.21

4.89 ± 0.435

5.475 ± 0.279

6.632 ± 0.266

6.738 ± 0.419

7.712 ± 0.259

1.383 ± 0.145

3.93 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski