Pelagibacter phage HTVC121P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; unclassified Pelagivirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NTE7|A0A4Y1NTE7_9CAUD Single-stranded DNA-binding protein OS=Pelagibacter phage HTVC121P OX=2283022 GN=P121_gp18 PE=4 SV=1
MM1 pKa = 7.74DD2 pKa = 3.96VSVMSDD8 pKa = 3.75EE9 pKa = 3.94FHH11 pKa = 6.86EE12 pKa = 4.53WLNQCPVQWFRR23 pKa = 11.84TGNGKK28 pKa = 9.69HH29 pKa = 5.11YY30 pKa = 11.04NEE32 pKa = 4.93DD33 pKa = 2.93KK34 pKa = 10.84SYY36 pKa = 11.33YY37 pKa = 9.83EE38 pKa = 4.1GASYY42 pKa = 10.24MFLKK46 pKa = 10.61NDD48 pKa = 3.31EE49 pKa = 5.09DD50 pKa = 4.09EE51 pKa = 5.61DD52 pKa = 4.69SEE54 pKa = 4.34EE55 pKa = 4.3DD56 pKa = 3.26

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NTD6|A0A4Y1NTD6_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC121P OX=2283022 GN=P121_gp03 PE=4 SV=1
MM1 pKa = 7.46ITRR4 pKa = 11.84KK5 pKa = 10.11DD6 pKa = 3.39WLKK9 pKa = 9.13TDD11 pKa = 2.83AGKK14 pKa = 10.57RR15 pKa = 11.84YY16 pKa = 9.25KK17 pKa = 10.85AKK19 pKa = 9.56TNKK22 pKa = 9.94NYY24 pKa = 10.04RR25 pKa = 11.84QKK27 pKa = 11.09KK28 pKa = 8.7KK29 pKa = 9.55QDD31 pKa = 3.12KK32 pKa = 10.36KK33 pKa = 11.23KK34 pKa = 10.1SLKK37 pKa = 9.68VNKK40 pKa = 9.73SFSFHH45 pKa = 6.48FPSEE49 pKa = 4.22EE50 pKa = 3.66PDD52 pKa = 3.11

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13385

39

1281

234.8

26.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.591 ± 0.543

0.926 ± 0.17

5.798 ± 0.19

6.642 ± 0.519

3.952 ± 0.189

6.53 ± 0.328

1.913 ± 0.22

6.597 ± 0.207

8.592 ± 0.645

7.919 ± 0.416

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.24

6.007 ± 0.448

2.973 ± 0.17

3.467 ± 0.218

3.758 ± 0.276

6.941 ± 0.413

7.142 ± 0.692

5.827 ± 0.398

1.375 ± 0.151

3.504 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski