Amino acid dipepetide frequency for Rhodobacteraceae bacterium KMS-5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.284AlaAla: 20.284 ± 0.212
1.223AlaCys: 1.223 ± 0.034
7.428AlaAsp: 7.428 ± 0.098
9.193AlaGlu: 9.193 ± 0.111
4.706AlaPhe: 4.706 ± 0.07
12.72AlaGly: 12.72 ± 0.131
2.691AlaHis: 2.691 ± 0.053
5.804AlaIle: 5.804 ± 0.085
4.075AlaLys: 4.075 ± 0.067
15.549AlaLeu: 15.549 ± 0.141
4.161AlaMet: 4.161 ± 0.063
2.79AlaAsn: 2.79 ± 0.054
6.252AlaPro: 6.252 ± 0.1
4.288AlaGln: 4.288 ± 0.07
10.264AlaArg: 10.264 ± 0.115
5.626AlaSer: 5.626 ± 0.07
6.316AlaThr: 6.316 ± 0.077
9.232AlaVal: 9.232 ± 0.113
1.426AlaTrp: 1.426 ± 0.038
2.51AlaTyr: 2.51 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
1.021CysAla: 1.021 ± 0.031
0.103CysCys: 0.103 ± 0.01
0.622CysAsp: 0.622 ± 0.023
0.414CysGlu: 0.414 ± 0.019
0.322CysPhe: 0.322 ± 0.015
0.966CysGly: 0.966 ± 0.035
0.271CysHis: 0.271 ± 0.016
0.385CysIle: 0.385 ± 0.018
0.217CysLys: 0.217 ± 0.015
0.911CysLeu: 0.911 ± 0.03
0.164CysMet: 0.164 ± 0.012
0.194CysAsn: 0.194 ± 0.013
0.557CysPro: 0.557 ± 0.023
0.245CysGln: 0.245 ± 0.016
0.548CysArg: 0.548 ± 0.023
0.412CysSer: 0.412 ± 0.02
0.47CysThr: 0.47 ± 0.023
0.572CysVal: 0.572 ± 0.024
0.109CysTrp: 0.109 ± 0.01
0.22CysTyr: 0.22 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
6.752AspAla: 6.752 ± 0.078
0.539AspCys: 0.539 ± 0.023
2.952AspAsp: 2.952 ± 0.067
3.134AspGlu: 3.134 ± 0.061
2.204AspPhe: 2.204 ± 0.051
5.263AspGly: 5.263 ± 0.09
1.344AspHis: 1.344 ± 0.034
2.628AspIle: 2.628 ± 0.052
1.528AspLys: 1.528 ± 0.043
6.732AspLeu: 6.732 ± 0.089
1.46AspMet: 1.46 ± 0.036
1.095AspAsn: 1.095 ± 0.031
3.555AspPro: 3.555 ± 0.063
1.842AspGln: 1.842 ± 0.042
4.459AspArg: 4.459 ± 0.069
2.117AspSer: 2.117 ± 0.047
2.597AspThr: 2.597 ± 0.063
3.702AspVal: 3.702 ± 0.055
1.279AspTrp: 1.279 ± 0.035
1.443AspTyr: 1.443 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
9.244GluAla: 9.244 ± 0.106
0.345GluCys: 0.345 ± 0.019
2.805GluAsp: 2.805 ± 0.048
2.949GluGlu: 2.949 ± 0.052
1.773GluPhe: 1.773 ± 0.038
4.963GluGly: 4.963 ± 0.063
0.993GluHis: 0.993 ± 0.03
3.443GluIle: 3.443 ± 0.06
1.957GluLys: 1.957 ± 0.047
4.605GluLeu: 4.605 ± 0.065
1.766GluMet: 1.766 ± 0.036
1.456GluAsn: 1.456 ± 0.035
2.463GluPro: 2.463 ± 0.045
1.553GluGln: 1.553 ± 0.037
3.923GluArg: 3.923 ± 0.063
1.839GluSer: 1.839 ± 0.042
3.602GluThr: 3.602 ± 0.055
4.631GluVal: 4.631 ± 0.065
0.676GluTrp: 0.676 ± 0.028
0.911GluTyr: 0.911 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.787PheAla: 4.787 ± 0.07
0.424PheCys: 0.424 ± 0.018
2.736PheAsp: 2.736 ± 0.052
1.858PheGlu: 1.858 ± 0.047
1.256PhePhe: 1.256 ± 0.042
3.838PheGly: 3.838 ± 0.065
0.777PheHis: 0.777 ± 0.024
1.453PheIle: 1.453 ± 0.034
0.917PheLys: 0.917 ± 0.031
3.545PheLeu: 3.545 ± 0.064
0.71PheMet: 0.71 ± 0.022
0.968PheAsn: 0.968 ± 0.031
1.617PhePro: 1.617 ± 0.032
1.026PheGln: 1.026 ± 0.032
2.278PheArg: 2.278 ± 0.042
1.951PheSer: 1.951 ± 0.041
2.109PheThr: 2.109 ± 0.04
2.567PheVal: 2.567 ± 0.048
0.552PheTrp: 0.552 ± 0.022
0.855PheTyr: 0.855 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
10.935GlyAla: 10.935 ± 0.116
0.887GlyCys: 0.887 ± 0.031
4.557GlyAsp: 4.557 ± 0.086
4.758GlyGlu: 4.758 ± 0.065
3.889GlyPhe: 3.889 ± 0.057
8.205GlyGly: 8.205 ± 0.126
2.14GlyHis: 2.14 ± 0.045
4.345GlyIle: 4.345 ± 0.068
3.443GlyLys: 3.443 ± 0.064
10.324GlyLeu: 10.324 ± 0.113
2.707GlyMet: 2.707 ± 0.055
2.343GlyAsn: 2.343 ± 0.08
4.053GlyPro: 4.053 ± 0.075
3.509GlyGln: 3.509 ± 0.058
6.428GlyArg: 6.428 ± 0.085
4.191GlySer: 4.191 ± 0.067
4.608GlyThr: 4.608 ± 0.083
6.482GlyVal: 6.482 ± 0.094
1.737GlyTrp: 1.737 ± 0.042
2.196GlyTyr: 2.196 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.557HisAla: 2.557 ± 0.047
0.241HisCys: 0.241 ± 0.015
1.288HisAsp: 1.288 ± 0.032
1.06HisGlu: 1.06 ± 0.031
0.839HisPhe: 0.839 ± 0.028
2.025HisGly: 2.025 ± 0.046
0.572HisHis: 0.572 ± 0.027
0.887HisIle: 0.887 ± 0.027
0.508HisLys: 0.508 ± 0.02
2.323HisLeu: 2.323 ± 0.047
0.542HisMet: 0.542 ± 0.021
0.427HisAsn: 0.427 ± 0.02
1.499HisPro: 1.499 ± 0.037
0.585HisGln: 0.585 ± 0.024
1.48HisArg: 1.48 ± 0.042
0.908HisSer: 0.908 ± 0.026
0.734HisThr: 0.734 ± 0.027
1.54HisVal: 1.54 ± 0.041
0.388HisTrp: 0.388 ± 0.019
0.535HisTyr: 0.535 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
6.942IleAla: 6.942 ± 0.083
0.525IleCys: 0.525 ± 0.023
3.283IleAsp: 3.283 ± 0.052
3.099IleGlu: 3.099 ± 0.064
1.602IlePhe: 1.602 ± 0.04
4.692IleGly: 4.692 ± 0.067
0.917IleHis: 0.917 ± 0.03
1.89IleIle: 1.89 ± 0.045
1.221IleLys: 1.221 ± 0.034
4.55IleLeu: 4.55 ± 0.08
0.924IleMet: 0.924 ± 0.027
1.166IleAsn: 1.166 ± 0.035
2.205IlePro: 2.205 ± 0.047
0.973IleGln: 0.973 ± 0.033
3.121IleArg: 3.121 ± 0.051
2.693IleSer: 2.693 ± 0.055
2.754IleThr: 2.754 ± 0.056
3.355IleVal: 3.355 ± 0.059
0.697IleTrp: 0.697 ± 0.028
1.042IleTyr: 1.042 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
4.533LysAla: 4.533 ± 0.067
0.168LysCys: 0.168 ± 0.014
1.727LysAsp: 1.727 ± 0.046
1.336LysGlu: 1.336 ± 0.044
0.822LysPhe: 0.822 ± 0.029
3.082LysGly: 3.082 ± 0.064
0.553LysHis: 0.553 ± 0.022
1.485LysIle: 1.485 ± 0.04
1.085LysLys: 1.085 ± 0.041
2.918LysLeu: 2.918 ± 0.053
0.8LysMet: 0.8 ± 0.028
0.702LysAsn: 0.702 ± 0.028
1.898LysPro: 1.898 ± 0.049
0.774LysGln: 0.774 ± 0.028
2.002LysArg: 2.002 ± 0.047
1.567LysSer: 1.567 ± 0.039
1.87LysThr: 1.87 ± 0.039
2.43LysVal: 2.43 ± 0.049
0.359LysTrp: 0.359 ± 0.018
0.576LysTyr: 0.576 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
15.621LeuAla: 15.621 ± 0.158
0.964LeuCys: 0.964 ± 0.029
5.794LeuAsp: 5.794 ± 0.068
5.157LeuGlu: 5.157 ± 0.066
3.304LeuPhe: 3.304 ± 0.061
8.991LeuGly: 8.991 ± 0.101
2.052LeuHis: 2.052 ± 0.043
5.001LeuIle: 5.001 ± 0.074
3.317LeuLys: 3.317 ± 0.059
9.061LeuLeu: 9.061 ± 0.141
2.794LeuMet: 2.794 ± 0.054
2.575LeuAsn: 2.575 ± 0.048
6.208LeuPro: 6.208 ± 0.085
2.873LeuGln: 2.873 ± 0.054
7.301LeuArg: 7.301 ± 0.081
6.116LeuSer: 6.116 ± 0.079
6.29LeuThr: 6.29 ± 0.067
7.377LeuVal: 7.377 ± 0.09
1.552LeuTrp: 1.552 ± 0.043
1.972LeuTyr: 1.972 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
4.081MetAla: 4.081 ± 0.062
0.16MetCys: 0.16 ± 0.012
1.307MetAsp: 1.307 ± 0.034
1.269MetGlu: 1.269 ± 0.036
0.767MetPhe: 0.767 ± 0.029
2.401MetGly: 2.401 ± 0.05
0.433MetHis: 0.433 ± 0.017
1.331MetIle: 1.331 ± 0.033
1.004MetLys: 1.004 ± 0.032
2.545MetLeu: 2.545 ± 0.057
0.701MetMet: 0.701 ± 0.028
0.759MetAsn: 0.759 ± 0.026
1.442MetPro: 1.442 ± 0.041
0.964MetGln: 0.964 ± 0.027
1.805MetArg: 1.805 ± 0.037
1.516MetSer: 1.516 ± 0.035
2.062MetThr: 2.062 ± 0.041
2.011MetVal: 2.011 ± 0.048
0.227MetTrp: 0.227 ± 0.014
0.273MetTyr: 0.273 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.071AsnAla: 3.071 ± 0.053
0.247AsnCys: 0.247 ± 0.014
1.372AsnAsp: 1.372 ± 0.055
0.948AsnGlu: 0.948 ± 0.031
0.903AsnPhe: 0.903 ± 0.027
2.269AsnGly: 2.269 ± 0.051
0.495AsnHis: 0.495 ± 0.021
1.155AsnIle: 1.155 ± 0.033
0.555AsnLys: 0.555 ± 0.024
2.541AsnLeu: 2.541 ± 0.047
0.604AsnMet: 0.604 ± 0.021
0.548AsnAsn: 0.548 ± 0.021
1.845AsnPro: 1.845 ± 0.038
0.613AsnGln: 0.613 ± 0.019
1.703AsnArg: 1.703 ± 0.037
1.043AsnSer: 1.043 ± 0.034
1.228AsnThr: 1.228 ± 0.034
1.658AsnVal: 1.658 ± 0.042
0.387AsnTrp: 0.387 ± 0.02
0.562AsnTyr: 0.562 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
6.916ProAla: 6.916 ± 0.092
0.362ProCys: 0.362 ± 0.02
3.717ProAsp: 3.717 ± 0.062
4.141ProGlu: 4.141 ± 0.061
2.006ProPhe: 2.006 ± 0.043
5.049ProGly: 5.049 ± 0.079
1.075ProHis: 1.075 ± 0.028
2.006ProIle: 2.006 ± 0.044
1.69ProLys: 1.69 ± 0.043
4.928ProLeu: 4.928 ± 0.069
1.486ProMet: 1.486 ± 0.037
1.189ProAsn: 1.189 ± 0.031
2.404ProPro: 2.404 ± 0.059
1.608ProGln: 1.608 ± 0.04
2.996ProArg: 2.996 ± 0.058
2.251ProSer: 2.251 ± 0.046
2.342ProThr: 2.342 ± 0.052
4.499ProVal: 4.499 ± 0.059
0.744ProTrp: 0.744 ± 0.028
1.095ProTyr: 1.095 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
4.33GlnAla: 4.33 ± 0.063
0.181GlnCys: 0.181 ± 0.014
1.566GlnAsp: 1.566 ± 0.037
1.321GlnGlu: 1.321 ± 0.035
0.954GlnPhe: 0.954 ± 0.031
2.961GlnGly: 2.961 ± 0.053
0.589GlnHis: 0.589 ± 0.022
1.884GlnIle: 1.884 ± 0.04
1.059GlnLys: 1.059 ± 0.03
2.634GlnLeu: 2.634 ± 0.046
1.004GlnMet: 1.004 ± 0.028
0.773GlnAsn: 0.773 ± 0.024
1.731GlnPro: 1.731 ± 0.038
0.797GlnGln: 0.797 ± 0.027
2.068GlnArg: 2.068 ± 0.049
1.653GlnSer: 1.653 ± 0.037
1.662GlnThr: 1.662 ± 0.039
2.454GlnVal: 2.454 ± 0.053
0.391GlnTrp: 0.391 ± 0.017
0.5GlnTyr: 0.5 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
9.232ArgAla: 9.232 ± 0.094
0.49ArgCys: 0.49 ± 0.022
3.91ArgAsp: 3.91 ± 0.066
3.782ArgGlu: 3.782 ± 0.056
2.774ArgPhe: 2.774 ± 0.049
4.908ArgGly: 4.908 ± 0.075
1.74ArgHis: 1.74 ± 0.041
3.74ArgIle: 3.74 ± 0.051
2.186ArgLys: 2.186 ± 0.052
8.35ArgLeu: 8.35 ± 0.107
1.974ArgMet: 1.974 ± 0.042
1.561ArgAsn: 1.561 ± 0.038
3.679ArgPro: 3.679 ± 0.057
2.405ArgGln: 2.405 ± 0.052
5.092ArgArg: 5.092 ± 0.085
3.018ArgSer: 3.018 ± 0.058
2.721ArgThr: 2.721 ± 0.046
4.825ArgVal: 4.825 ± 0.068
0.978ArgTrp: 0.978 ± 0.033
1.437ArgTyr: 1.437 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
5.698SerAla: 5.698 ± 0.076
0.417SerCys: 0.417 ± 0.019
2.715SerAsp: 2.715 ± 0.048
2.373SerGlu: 2.373 ± 0.049
2.086SerPhe: 2.086 ± 0.046
5.166SerGly: 5.166 ± 0.075
1.105SerHis: 1.105 ± 0.034
2.097SerIle: 2.097 ± 0.045
1.22SerLys: 1.22 ± 0.034
5.009SerLeu: 5.009 ± 0.078
1.142SerMet: 1.142 ± 0.033
1.133SerAsn: 1.133 ± 0.033
2.56SerPro: 2.56 ± 0.046
1.416SerGln: 1.416 ± 0.036
3.208SerArg: 3.208 ± 0.061
2.188SerSer: 2.188 ± 0.044
2.252SerThr: 2.252 ± 0.041
3.359SerVal: 3.359 ± 0.057
0.646SerTrp: 0.646 ± 0.026
1.245SerTyr: 1.245 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
6.747ThrAla: 6.747 ± 0.085
0.499ThrCys: 0.499 ± 0.024
2.915ThrAsp: 2.915 ± 0.05
2.806ThrGlu: 2.806 ± 0.05
1.926ThrPhe: 1.926 ± 0.045
5.7ThrGly: 5.7 ± 0.086
1.045ThrHis: 1.045 ± 0.032
2.53ThrIle: 2.53 ± 0.048
1.294ThrLys: 1.294 ± 0.039
6.076ThrLeu: 6.076 ± 0.088
1.194ThrMet: 1.194 ± 0.03
1.149ThrAsn: 1.149 ± 0.033
3.23ThrPro: 3.23 ± 0.057
1.363ThrGln: 1.363 ± 0.034
3.371ThrArg: 3.371 ± 0.06
2.356ThrSer: 2.356 ± 0.049
2.68ThrThr: 2.68 ± 0.05
4.218ThrVal: 4.218 ± 0.062
0.65ThrTrp: 0.65 ± 0.022
1.137ThrTyr: 1.137 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
9.665ValAla: 9.665 ± 0.103
0.595ValCys: 0.595 ± 0.022
3.764ValAsp: 3.764 ± 0.053
4.503ValGlu: 4.503 ± 0.069
2.721ValPhe: 2.721 ± 0.049
5.358ValGly: 5.358 ± 0.072
1.271ValHis: 1.271 ± 0.036
4.108ValIle: 4.108 ± 0.067
2.379ValLys: 2.379 ± 0.05
7.758ValLeu: 7.758 ± 0.096
2.088ValMet: 2.088 ± 0.045
1.964ValAsn: 1.964 ± 0.038
3.512ValPro: 3.512 ± 0.057
2.313ValGln: 2.313 ± 0.042
4.174ValArg: 4.174 ± 0.054
3.749ValSer: 3.749 ± 0.054
4.871ValThr: 4.871 ± 0.066
5.66ValVal: 5.66 ± 0.081
1.038ValTrp: 1.038 ± 0.032
1.45ValTyr: 1.45 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.602TrpAla: 1.602 ± 0.038
0.128TrpCys: 0.128 ± 0.01
0.706TrpAsp: 0.706 ± 0.029
0.666TrpGlu: 0.666 ± 0.025
0.567TrpPhe: 0.567 ± 0.023
1.136TrpGly: 1.136 ± 0.03
0.385TrpHis: 0.385 ± 0.018
0.645TrpIle: 0.645 ± 0.025
0.469TrpLys: 0.469 ± 0.023
1.734TrpLeu: 1.734 ± 0.045
0.391TrpMet: 0.391 ± 0.019
0.446TrpAsn: 0.446 ± 0.021
0.748TrpPro: 0.748 ± 0.027
0.706TrpGln: 0.706 ± 0.025
1.084TrpArg: 1.084 ± 0.037
0.786TrpSer: 0.786 ± 0.027
0.75TrpThr: 0.75 ± 0.026
0.967TrpVal: 0.967 ± 0.029
0.228TrpTrp: 0.228 ± 0.015
0.265TrpTyr: 0.265 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.521TyrAla: 2.521 ± 0.044
0.222TyrCys: 0.222 ± 0.014
1.464TyrAsp: 1.464 ± 0.037
1.128TyrGlu: 1.128 ± 0.033
0.805TyrPhe: 0.805 ± 0.029
2.043TyrGly: 2.043 ± 0.043
0.491TyrHis: 0.491 ± 0.025
0.833TyrIle: 0.833 ± 0.03
0.562TyrLys: 0.562 ± 0.021
2.226TyrLeu: 2.226 ± 0.046
0.444TyrMet: 0.444 ± 0.019
0.51TyrAsn: 0.51 ± 0.019
1.047TyrPro: 1.047 ± 0.029
0.659TyrGln: 0.659 ± 0.025
1.497TyrArg: 1.497 ± 0.035
1.054TyrSer: 1.054 ± 0.033
0.968TyrThr: 0.968 ± 0.032
1.406TyrVal: 1.406 ± 0.039
0.343TyrTrp: 0.343 ± 0.019
0.54TyrTyr: 0.54 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3633 proteins (1152034 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski