Siphoviridae sp. ctvD11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W5N6|A0A5Q2W5N6_9CAUD Uncharacterized protein OS=Siphoviridae sp. ctvD11 OX=2656724 PE=4 SV=1
MM1 pKa = 7.48SNEE4 pKa = 3.76TVATVATATNTTQTAEE20 pKa = 4.03LAYY23 pKa = 11.28NMASPSSGLRR33 pKa = 11.84FVIASLDD40 pKa = 3.64TLPVLRR46 pKa = 11.84LTVSNLVSDD55 pKa = 4.1PTGSSVVFSMKK66 pKa = 10.49NADD69 pKa = 3.63TGVVTVSRR77 pKa = 11.84ASAQIGSITSASSLYY92 pKa = 10.1TCILSYY98 pKa = 10.5TFSAVTSATPGIYY111 pKa = 9.59EE112 pKa = 4.32AEE114 pKa = 4.31FEE116 pKa = 4.44VTLPGGGIITIPNDD130 pKa = 3.45PKK132 pKa = 11.16YY133 pKa = 10.39PITVVIKK140 pKa = 10.96NDD142 pKa = 3.11IAA144 pKa = 5.48

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W8E1|A0A5Q2W8E1_9CAUD Uncharacterized protein OS=Siphoviridae sp. ctvD11 OX=2656724 PE=4 SV=1
MM1 pKa = 6.9GTVGNSGRR9 pKa = 11.84GCPRR13 pKa = 11.84VYY15 pKa = 10.24RR16 pKa = 11.84QPEE19 pKa = 4.12TQRR22 pKa = 11.84KK23 pKa = 8.0EE24 pKa = 4.21PEE26 pKa = 3.69TSLPRR31 pKa = 11.84EE32 pKa = 4.08TPGRR36 pKa = 11.84KK37 pKa = 8.94

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

8802

37

1084

191.3

21.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.169 ± 0.595

1.068 ± 0.157

5.771 ± 0.237

5.987 ± 0.575

3.647 ± 0.226

6.601 ± 0.571

2.011 ± 0.207

5.726 ± 0.304

6.033 ± 0.518

9.452 ± 0.5

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.25

4.135 ± 0.345

4.101 ± 0.337

3.454 ± 0.197

4.465 ± 0.436

6.499 ± 0.329

8.805 ± 0.701

6.76 ± 0.334

1.318 ± 0.108

3.658 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski