Streptococcus phage phiARI0462

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141E012|A0A141E012_9CAUD Uncharacterized protein OS=Streptococcus phage phiARI0462 OX=1701826 GN=phiARI0462_41 PE=4 SV=1
MM1 pKa = 8.35LMFDD5 pKa = 3.85YY6 pKa = 10.77DD7 pKa = 4.39RR8 pKa = 11.84DD9 pKa = 3.49IMQPPEE15 pKa = 3.91PRR17 pKa = 11.84EE18 pKa = 3.89EE19 pKa = 4.47HH20 pKa = 7.45DD21 pKa = 3.52PADD24 pKa = 4.15WIFSAGQWIYY34 pKa = 11.59VGDD37 pKa = 4.05CC38 pKa = 3.39

Molecular weight: 4.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141E023|A0A141E023_9CAUD Integrase OS=Streptococcus phage phiARI0462 OX=1701826 GN=phiARI0462_52 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 9.96FQQGEE7 pKa = 4.43VYY9 pKa = 10.72LINFPQKK16 pKa = 10.5GGNEE20 pKa = 4.58FYY22 pKa = 10.56GKK24 pKa = 9.64HH25 pKa = 4.67YY26 pKa = 10.65AIILTTPDD34 pKa = 3.61KK35 pKa = 11.45VDD37 pKa = 3.23GTLLVAPLTGKK48 pKa = 10.64KK49 pKa = 9.69SGKK52 pKa = 9.46KK53 pKa = 8.13YY54 pKa = 10.33RR55 pKa = 11.84GGITIEE61 pKa = 3.65NSKK64 pKa = 9.7YY65 pKa = 10.31QNTPSKK71 pKa = 9.27PKK73 pKa = 9.77AYY75 pKa = 10.12AYY77 pKa = 9.91VRR79 pKa = 11.84KK80 pKa = 9.47IQEE83 pKa = 3.23IDD85 pKa = 3.01KK86 pKa = 10.87RR87 pKa = 11.84KK88 pKa = 9.65IVYY91 pKa = 7.82KK92 pKa = 9.5TKK94 pKa = 10.76KK95 pKa = 8.95KK96 pKa = 9.06TDD98 pKa = 3.08SDD100 pKa = 4.19GQVMLDD106 pKa = 3.32AAGKK110 pKa = 8.79EE111 pKa = 4.41LYY113 pKa = 10.61DD114 pKa = 3.53KK115 pKa = 10.85VYY117 pKa = 10.73RR118 pKa = 11.84PAYY121 pKa = 9.72KK122 pKa = 10.41LDD124 pKa = 3.84TNDD127 pKa = 4.16HH128 pKa = 6.5KK129 pKa = 11.5KK130 pKa = 10.69LLDD133 pKa = 4.17KK134 pKa = 10.82IKK136 pKa = 10.64EE137 pKa = 4.04VLGLDD142 pKa = 4.25LYY144 pKa = 11.51

Molecular weight: 16.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11081

32

2146

217.3

24.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.211 ± 0.526

0.623 ± 0.103

6.48 ± 0.347

8.086 ± 0.557

4.025 ± 0.27

6.606 ± 0.592

1.272 ± 0.195

6.353 ± 0.238

8.609 ± 0.417

8.041 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.215

5.252 ± 0.324

2.572 ± 0.257

4.431 ± 0.249

4.44 ± 0.345

6.064 ± 0.477

6.128 ± 0.416

6.173 ± 0.328

1.435 ± 0.175

3.826 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski