Ceraceosorus guamensis
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7822 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316VYH5|A0A316VYH5_9BASI Uncharacterized protein OS=Ceraceosorus guamensis OX=1522189 GN=IE81DRAFT_134927 PE=4 SV=1
MM1 pKa = 7.86 PKK3 pKa = 10.06 QSIYY7 pKa = 11.28 DD8 pKa = 3.78 EE9 pKa = 4.68 VEE11 pKa = 4.13 LEE13 pKa = 4.14 DD14 pKa = 3.58 MVYY17 pKa = 10.6 DD18 pKa = 3.75 AEE20 pKa = 5.23 KK21 pKa = 11.2 DD22 pKa = 3.6 LFHH25 pKa = 6.67 YY26 pKa = 7.91 PCPCGDD32 pKa = 3.14 RR33 pKa = 11.84 FEE35 pKa = 4.24 ITRR38 pKa = 11.84 AQLKK42 pKa = 8.89 EE43 pKa = 4.05 AEE45 pKa = 5.31 DD46 pKa = 3.62 IATCPSCSLLIRR58 pKa = 11.84 VVFDD62 pKa = 4.08 PLDD65 pKa = 3.97 FEE67 pKa = 7.56 DD68 pKa = 6.05 DD69 pKa = 4.18 DD70 pKa = 6.04 DD71 pKa = 6.17 SEE73 pKa = 6.03 APALPAHH80 pKa = 6.44 SPLDD84 pKa = 3.5 QVSQVATAAA93 pKa = 3.59
Molecular weight: 10.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.164
Thurlkill 3.681
EMBOSS 3.77
Sillero 3.948
Patrickios 1.901
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A316VTA1|A0A316VTA1_9BASI SH3 domain-containing protein OS=Ceraceosorus guamensis OX=1522189 GN=IE81DRAFT_327191 PE=4 SV=1
MM1 pKa = 7.94 PSQKK5 pKa = 10.45 SFRR8 pKa = 11.84 TKK10 pKa = 10.79 SKK12 pKa = 9.4 LAKK15 pKa = 9.87 AARR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPSWFRR28 pKa = 11.84 MKK30 pKa = 10.03 TGNKK34 pKa = 8.05 IQYY37 pKa = 7.53 NAKK40 pKa = 8.43 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.03 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 10.83 LGLL51 pKa = 3.67
Molecular weight: 6.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 11.008
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7822
0
7822
4077528
49
4808
521.3
56.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.389 ± 0.029
1.091 ± 0.012
5.414 ± 0.016
5.86 ± 0.022
3.005 ± 0.017
7.345 ± 0.027
2.573 ± 0.014
3.755 ± 0.018
4.155 ± 0.022
8.966 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.815 ± 0.009
2.899 ± 0.012
6.108 ± 0.028
4.167 ± 0.019
7.081 ± 0.026
9.865 ± 0.043
5.539 ± 0.014
5.688 ± 0.018
1.26 ± 0.009
2.024 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here