Vibrio phage phi50-12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; unclassified Schitoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8PR76|A0A5P8PR76_9CAUD Uncharacterized protein OS=Vibrio phage phi50-12 OX=2654972 GN=VOWphi5012_020 PE=4 SV=1
MM1 pKa = 7.28TLDD4 pKa = 5.26DD5 pKa = 5.13IYY7 pKa = 11.88NLLTSGEE14 pKa = 4.14LRR16 pKa = 11.84QHH18 pKa = 6.46PLFANGLKK26 pKa = 10.81DD27 pKa = 3.56EE28 pKa = 5.23DD29 pKa = 4.15KK30 pKa = 11.23EE31 pKa = 4.44KK32 pKa = 11.22VNQFITAGLTDD43 pKa = 4.92LYY45 pKa = 11.12TRR47 pKa = 11.84FPLKK51 pKa = 9.59TDD53 pKa = 3.46RR54 pKa = 11.84LVLVMDD60 pKa = 5.46EE61 pKa = 4.58IEE63 pKa = 4.26TVYY66 pKa = 10.76PLVQGSPHH74 pKa = 7.05IDD76 pKa = 3.25EE77 pKa = 5.28GLIPYY82 pKa = 8.96TGDD85 pKa = 3.29LLKK88 pKa = 10.93VLEE91 pKa = 4.29VLDD94 pKa = 4.49EE95 pKa = 4.69GEE97 pKa = 4.42CTVPLNNGNEE107 pKa = 4.06PCGVFTSSTNVLQIPQPVDD126 pKa = 2.92GDD128 pKa = 3.81MLYY131 pKa = 8.57ITYY134 pKa = 9.9QANHH138 pKa = 6.69KK139 pKa = 10.7AIDD142 pKa = 3.68TGIEE146 pKa = 3.98LPLNLVPALCAYY158 pKa = 9.85VGYY161 pKa = 10.31RR162 pKa = 11.84LYY164 pKa = 11.19SGDD167 pKa = 3.81TNQQSSVKK175 pKa = 10.19ANQLLSEE182 pKa = 4.32YY183 pKa = 10.18EE184 pKa = 4.19RR185 pKa = 11.84QCMNQEE191 pKa = 3.88LFGLVNKK198 pKa = 10.61DD199 pKa = 3.47DD200 pKa = 4.01VTTLTSFCTGGWVV213 pKa = 3.06

Molecular weight: 23.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8PRA8|A0A5P8PRA8_9CAUD Uncharacterized protein OS=Vibrio phage phi50-12 OX=2654972 GN=VOWphi5012_043 PE=4 SV=1
MM1 pKa = 7.58ARR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.37RR6 pKa = 11.84KK7 pKa = 9.69LVIKK11 pKa = 9.96VGNSIHH17 pKa = 6.91RR18 pKa = 11.84GNHH21 pKa = 4.28LRR23 pKa = 11.84VTIRR27 pKa = 11.84EE28 pKa = 4.37LYY30 pKa = 10.28GKK32 pKa = 8.63TITVIANVNGHH43 pKa = 5.87VSTDD47 pKa = 3.56DD48 pKa = 3.43NPFLVKK54 pKa = 10.36YY55 pKa = 9.81LKK57 pKa = 10.65NYY59 pKa = 8.66SHH61 pKa = 6.77NKK63 pKa = 8.95YY64 pKa = 10.09VLSPNEE70 pKa = 3.53YY71 pKa = 10.57DD72 pKa = 4.82IILEE76 pKa = 4.24KK77 pKa = 10.67PLGGSNAA84 pKa = 3.59

Molecular weight: 9.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

21516

42

3024

213.0

23.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.893 ± 0.393

1.032 ± 0.169

6.321 ± 0.231

7.469 ± 0.307

4.011 ± 0.199

6.102 ± 0.282

1.906 ± 0.169

6.149 ± 0.345

7.487 ± 0.3

8.45 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.161

5.81 ± 0.201

3.593 ± 0.218

4.016 ± 0.277

3.727 ± 0.149

6.102 ± 0.236

6.465 ± 0.265

6.73 ± 0.237

0.957 ± 0.09

3.955 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski