Arthrobotrys oligospora (strain ATCC 24927 / CBS 115.81 / DSM 1491) (Nematode-trapping fungus) (Didymozoophaga oligospora)
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11478 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1XPG7|G1XPG7_ARTOA Uncharacterized protein OS=Arthrobotrys oligospora (strain ATCC 24927 / CBS 115.81 / DSM 1491) OX=756982 GN=AOL_s00173g335 PE=4 SV=1
MM1 pKa = 7.46 LRR3 pKa = 11.84 LILSSLLVLGAAAVPAAKK21 pKa = 9.93 EE22 pKa = 3.66 LSEE25 pKa = 4.25 RR26 pKa = 11.84 QLPTYY31 pKa = 7.04 TLKK34 pKa = 9.75 VTGSGIAADD43 pKa = 3.98 TFLKK47 pKa = 9.14 ITEE50 pKa = 4.18 VDD52 pKa = 3.25 GTNYY56 pKa = 10.23 VGLVPSTDD64 pKa = 3.14 ASTFSYY70 pKa = 10.92 DD71 pKa = 3.09 AATSEE76 pKa = 4.61 LSAVDD81 pKa = 3.86 DD82 pKa = 4.09 AAVTLFSQSFLNQGDD97 pKa = 4.16 VYY99 pKa = 10.7 EE100 pKa = 4.32 EE101 pKa = 4.25 TPAPVSFRR109 pKa = 11.84 NEE111 pKa = 3.32 TDD113 pKa = 2.78 INGCVDD119 pKa = 3.29 TNEE122 pKa = 4.9 CGFEE126 pKa = 4.08 SWTLDD131 pKa = 3.7 PASGALGVVRR141 pKa = 11.84 TTSPNLYY148 pKa = 10.18 ACDD151 pKa = 5.1 DD152 pKa = 3.91 GAQYY156 pKa = 10.93 LLWIGPTTWVTTFCTPISLSAVV178 pKa = 3.33
Molecular weight: 18.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.821
IPC_protein 3.783
Toseland 3.579
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.49
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.91
Patrickios 0.604
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|G1X9U8|G1X9U8_ARTOA F-box domain-containing protein OS=Arthrobotrys oligospora (strain ATCC 24927 / CBS 115.81 / DSM 1491) OX=756982 GN=AOL_s00076g437 PE=4 SV=1
MM1 pKa = 7.74 TILTLLRR8 pKa = 11.84 RR9 pKa = 11.84 PAISCLRR16 pKa = 11.84 STASSSSRR24 pKa = 11.84 RR25 pKa = 11.84 TFTSLLRR32 pKa = 11.84 TPTTIITLQTRR43 pKa = 11.84 PTLHH47 pKa = 6.8 SSPTSLIPQTTSSTSQTTSTSLLLPLQGLTQTRR80 pKa = 11.84 GHH82 pKa = 6.29 RR83 pKa = 11.84 RR84 pKa = 11.84 KK85 pKa = 8.38 TYY87 pKa = 8.98 NPSHH91 pKa = 7.03 IVRR94 pKa = 11.84 KK95 pKa = 9.74 RR96 pKa = 11.84 RR97 pKa = 11.84 FGFLARR103 pKa = 11.84 LRR105 pKa = 11.84 SKK107 pKa = 9.3 TGKK110 pKa = 10.27 NILKK114 pKa = 9.96 RR115 pKa = 11.84 RR116 pKa = 11.84 KK117 pKa = 9.1 AKK119 pKa = 8.97 GRR121 pKa = 11.84 KK122 pKa = 8.03 MLTHH126 pKa = 7.27
Molecular weight: 14.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.082
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11478
0
11478
5722854
49
12515
498.6
55.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.544 ± 0.022
1.21 ± 0.009
5.649 ± 0.015
6.632 ± 0.027
3.725 ± 0.015
6.843 ± 0.022
2.179 ± 0.009
5.42 ± 0.017
5.543 ± 0.021
8.53 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.019 ± 0.009
4.093 ± 0.013
6.176 ± 0.027
3.822 ± 0.018
5.768 ± 0.019
8.351 ± 0.028
6.337 ± 0.026
5.881 ± 0.017
1.363 ± 0.009
2.915 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here