Chlamydiales bacterium SCGC AB-751-O23
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 876 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A212KPT4|A0A212KPT4_9CHLA Uncharacterized protein OS=Chlamydiales bacterium SCGC AB-751-O23 OX=1871322 GN=AB751O23_AJ_00140 PE=4 SV=1
MM1 pKa = 7.39 EE2 pKa = 5.71 HH3 pKa = 6.95 LSDD6 pKa = 3.95 YY7 pKa = 10.36 LTIEE11 pKa = 3.92 ALIEE15 pKa = 4.0 KK16 pKa = 10.22 EE17 pKa = 3.89 KK18 pKa = 10.98 FQDD21 pKa = 4.0 NFQLCNYY28 pKa = 9.73 AIQQVQNVVNVGNDD42 pKa = 3.07 NFDD45 pKa = 3.62 EE46 pKa = 5.0 NEE48 pKa = 4.18 IQNKK52 pKa = 9.64 AYY54 pKa = 10.76 NILDD58 pKa = 4.82 GILHH62 pKa = 6.64 DD63 pKa = 4.39 RR64 pKa = 11.84 DD65 pKa = 3.55 FAEE68 pKa = 4.78 EE69 pKa = 3.83 FAEE72 pKa = 4.42 SEE74 pKa = 4.65 GSADD78 pKa = 3.6 YY79 pKa = 11.17 QNSSEE84 pKa = 4.22 GNN86 pKa = 3.34
Molecular weight: 9.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.91
IPC_protein 3.834
Toseland 3.643
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.668
Grimsley 3.567
Solomon 3.783
Lehninger 3.732
Nozaki 3.923
DTASelect 4.075
Thurlkill 3.694
EMBOSS 3.719
Sillero 3.948
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A212KPG2|A0A212KPG2_9CHLA Elongation factor G OS=Chlamydiales bacterium SCGC AB-751-O23 OX=1871322 GN=fusA PE=3 SV=1
MM1 pKa = 7.63 VDD3 pKa = 3.56 LAKK6 pKa = 10.88 VNSSGSLIRR15 pKa = 11.84 EE16 pKa = 3.89 SWEE19 pKa = 3.61 LMRR22 pKa = 11.84 NLHH25 pKa = 5.25 SVRR28 pKa = 11.84 GRR30 pKa = 11.84 RR31 pKa = 11.84 FQSFRR36 pKa = 11.84 YY37 pKa = 8.91 LVCPMNALALVAAGLVGRR55 pKa = 11.84 ILGKK59 pKa = 10.05 MILSDD64 pKa = 3.47 KK65 pKa = 10.88 LYY67 pKa = 9.58 VQIFAFTVFLTLGLGTFSISRR88 pKa = 11.84 IFQSRR93 pKa = 11.84 IEE95 pKa = 4.01 TLKK98 pKa = 10.8 RR99 pKa = 11.84 SLHH102 pKa = 6.24 GGDD105 pKa = 3.5 KK106 pKa = 10.9
Molecular weight: 11.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.853
IPC_protein 10.906
Toseland 11.067
ProMoST 11.125
Dawson 11.111
Bjellqvist 10.921
Wikipedia 11.418
Rodwell 11.213
Grimsley 11.155
Solomon 11.374
Lehninger 11.316
Nozaki 11.052
DTASelect 10.921
Thurlkill 11.052
EMBOSS 11.506
Sillero 11.067
Patrickios 10.979
IPC_peptide 11.389
IPC2_peptide 10.131
IPC2.peptide.svr19 8.707
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
876
0
876
306603
30
5523
350.0
39.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.165 ± 0.08
1.047 ± 0.041
5.243 ± 0.123
7.597 ± 0.128
5.453 ± 0.088
5.895 ± 0.128
1.825 ± 0.049
6.763 ± 0.083
8.391 ± 0.169
11.395 ± 0.128
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.906 ± 0.046
4.852 ± 0.07
3.499 ± 0.076
3.599 ± 0.076
3.696 ± 0.066
8.301 ± 0.086
4.955 ± 0.149
5.531 ± 0.072
0.805 ± 0.027
3.083 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here