Carnation mottle virus (CarMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus

Average proteome isoelectric point is 8.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9WJE0|Q9WJE0_CARMV RNA-directed RNA polymerase OS=Carnation mottle virus OX=11986 PE=4 SV=1
MGLPSLLVEGVIGCTLVGGLVAVGSAALAVRATIGVVEFNRECVRGARRIVSSGGRCLVVQSPYGNPNQGLIRGEDEEIDNVEESTPVELTPLIEVKAEVDGKEVVVSKKRVVNRHLRQRFVRSIAIEAKNHFGGDISPSKANYLSVSKFLTGKCKERHVVPAHTRDCVSAAMVLVFTPDVHEIRMMAGLASDAAYGIKIAMASILNRKGWCWRLMVNPLDRARWWEMWCVVNGFDSNKPVTFPKXGGLFYLNGVETKIRRGGHPSVIEVDGQCPLKERKLYVQNAITTGYEYRVHNHSYANLRRGLLERVFYVERNKELVSCPQPEPGSFKEMGYLRRRFHRVCGNHTRISANDLVDCYQGRKRTIYENAAASLLDRAIERKDGDLKTFIKAEKFNVNLKSDPAPRVIQPRSPRYNVELGRYLKKYEHHAYKALDKIWGGPTVMKGYTTEEVAQHIWSAWNQFQTPVAIGFDMSRFDQHVSVAALEFEHSCYLACFEGDAHLANLLKMQLVNHGVGFASNGMLRYTKEGCRMSGDMNTALGNCLLACLITKHLMKIRSRLINNGDDCVLICERTDIDYVVSNLTTGWSRFGFNCIAEEPVYEMEKIRFCQMAPVFDGAGWLMVRDPLVSMSKDSHSLVHWNNETNAKQWLKSVGMCGLRIAGGVPVVQEFYQKYVETAGNVRENKNITEKSSSGFFMMADRAKRGYSAVSEVCRFSFYQAFGITPDQQIALEGEIRSLTINTNVGPQCEAADSLWILNRKYQ

Molecular weight: 85.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9WJE0|Q9WJE0_CARMV RNA-directed RNA polymerase OS=Carnation mottle virus OX=11986 PE=4 SV=1
MM1 pKa = 7.72PSANLHH7 pKa = 6.38LIVLTGVIGLMLLIRR22 pKa = 11.84LRR24 pKa = 11.84CTFTSTFSLPPLVTLNQIIALSFCGLLLNSISRR57 pKa = 11.84AEE59 pKa = 3.86RR60 pKa = 11.84ACYY63 pKa = 10.16YY64 pKa = 10.23NYY66 pKa = 10.88SVDD69 pKa = 3.47SSKK72 pKa = 10.36QQHH75 pKa = 6.02ISISTPNGKK84 pKa = 9.32

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2019

61

763

403.8

45.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.281 ± 0.273

2.576 ± 0.425

4.26 ± 0.374

5.844 ± 0.691

3.715 ± 0.075

7.776 ± 0.31

2.229 ± 0.401

5.201 ± 0.458

5.745 ± 0.408

7.826 ± 0.798

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.106

5.201 ± 0.301

4.16 ± 0.465

3.071 ± 0.167

6.835 ± 0.456

6.488 ± 0.686

5.102 ± 1.09

9.113 ± 0.398

1.535 ± 0.185

3.17 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski