Synechococcus phage S-CAM9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Kanaloavirus; Synechococcus virus SCAM9

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8KPM3|A0A1D8KPM3_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM9 OX=1883369 GN=N161109_012 PE=4 SV=1
MM1 pKa = 7.22SQPSQYY7 pKa = 10.95NPALTRR13 pKa = 11.84VYY15 pKa = 10.38TNSGDD20 pKa = 3.5NGTIEE25 pKa = 4.0MMMPGLDD32 pKa = 3.48SRR34 pKa = 11.84VEE36 pKa = 4.71DD37 pKa = 4.19IVHH40 pKa = 6.38MDD42 pKa = 3.38YY43 pKa = 11.35DD44 pKa = 3.63AGTRR48 pKa = 11.84FWIVTYY54 pKa = 8.79DD55 pKa = 3.44TVEE58 pKa = 4.09EE59 pKa = 4.47MVQDD63 pKa = 4.03MPDD66 pKa = 3.26TDD68 pKa = 4.01FWAAITVDD76 pKa = 4.06EE77 pKa = 4.85DD78 pKa = 4.75AMGEE82 pKa = 4.08PTGVSMGEE90 pKa = 3.81DD91 pKa = 3.65AYY93 pKa = 11.46VALHH97 pKa = 6.17PEE99 pKa = 4.34CSRR102 pKa = 11.84YY103 pKa = 10.05

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8KNK8|A0A1D8KNK8_9CAUD Baseplate wedge initiator OS=Synechococcus phage S-CAM9 OX=1883369 GN=N161109_102 PE=4 SV=1
MM1 pKa = 7.46NNDD4 pKa = 2.69QRR6 pKa = 11.84IRR8 pKa = 11.84SNLAAFGEE16 pKa = 4.88NYY18 pKa = 8.59TPPFNWSQSEE28 pKa = 4.06NFGQGACLTAKK39 pKa = 9.9QVEE42 pKa = 4.33KK43 pKa = 10.55RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84ALWTAA52 pKa = 3.43

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

54388

37

3884

237.5

26.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.988 ± 0.193

0.941 ± 0.091

6.577 ± 0.107

6.45 ± 0.277

4.56 ± 0.139

7.531 ± 0.361

1.693 ± 0.122

6.533 ± 0.194

6.156 ± 0.304

7.18 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.155

5.738 ± 0.19

4.067 ± 0.158

3.672 ± 0.094

4.374 ± 0.129

6.952 ± 0.238

7.195 ± 0.428

6.952 ± 0.192

1.214 ± 0.102

4.227 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski