Bacillus phage vB_BpuM-BpSp

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 318 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141HRP3|A0A141HRP3_9CAUD Uncharacterized protein OS=Bacillus phage vB_BpuM-BpSp OX=1739968 GN=Bp8pS_002 PE=4 SV=1
MM1 pKa = 7.58EE2 pKa = 4.62MGKK5 pKa = 9.67VEE7 pKa = 4.27VVKK10 pKa = 10.84SLEE13 pKa = 3.75KK14 pKa = 10.83FEE16 pKa = 5.21IIRR19 pKa = 11.84LYY21 pKa = 11.03SDD23 pKa = 4.36AGLKK27 pKa = 10.48EE28 pKa = 4.32NGDD31 pKa = 3.77PYY33 pKa = 9.89YY34 pKa = 10.58QNVYY38 pKa = 10.02ISEE41 pKa = 4.18DD42 pKa = 3.38QVRR45 pKa = 11.84EE46 pKa = 3.8YY47 pKa = 10.72HH48 pKa = 6.83PEE50 pKa = 3.73VEE52 pKa = 4.99LIISGYY58 pKa = 10.53GFIDD62 pKa = 4.24KK63 pKa = 8.27EE64 pKa = 4.19TGYY67 pKa = 10.57QPEE70 pKa = 4.16NTPDD74 pKa = 2.78WFYY77 pKa = 11.26STDD80 pKa = 3.28EE81 pKa = 4.07VEE83 pKa = 5.88AFIEE87 pKa = 4.16KK88 pKa = 10.52NYY90 pKa = 11.02

Molecular weight: 10.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141HSE4|A0A141HSE4_9CAUD Uncharacterized protein OS=Bacillus phage vB_BpuM-BpSp OX=1739968 GN=Bp8pS_253 PE=4 SV=1
MM1 pKa = 7.29TSKK4 pKa = 9.44KK5 pKa = 6.78TTAQFRR11 pKa = 11.84QEE13 pKa = 3.97VFNLVGSEE21 pKa = 4.04YY22 pKa = 10.64KK23 pKa = 10.54VLYY26 pKa = 9.76EE27 pKa = 3.85YY28 pKa = 10.85RR29 pKa = 11.84LSNVRR34 pKa = 11.84IRR36 pKa = 11.84FRR38 pKa = 11.84HH39 pKa = 4.68MVCKK43 pKa = 9.99RR44 pKa = 11.84DD45 pKa = 4.02FSRR48 pKa = 11.84TPSEE52 pKa = 4.18FLSKK56 pKa = 9.47TRR58 pKa = 11.84NLKK61 pKa = 10.5CPYY64 pKa = 9.65CKK66 pKa = 10.27NNKK69 pKa = 8.85

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

318

0

318

77933

30

1797

245.1

28.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.818 ± 0.135

0.518 ± 0.037

6.844 ± 0.111

8.512 ± 0.182

5.194 ± 0.096

3.834 ± 0.184

1.363 ± 0.061

9.841 ± 0.155

10.69 ± 0.185

9.336 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.058 ± 0.053

8.697 ± 0.14

2.26 ± 0.081

2.216 ± 0.06

3.531 ± 0.069

7.124 ± 0.177

4.655 ± 0.132

4.799 ± 0.12

0.689 ± 0.043

5.02 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski