Streptococcus satellite phage Javan203

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZGL3|A0A4D5ZGL3_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan203 OX=2558565 GN=JavanS203_0003 PE=4 SV=1
MM1 pKa = 6.67KK2 pKa = 10.13TSVGLLLDD10 pKa = 4.17ALSEE14 pKa = 4.31IVPNWDD20 pKa = 3.0IEE22 pKa = 4.1DD23 pKa = 3.68RR24 pKa = 11.84LYY26 pKa = 11.49SSVVKK31 pKa = 10.0MIKK34 pKa = 9.82EE35 pKa = 3.94LPKK38 pKa = 9.11EE39 pKa = 3.59DD40 pKa = 3.87SYY42 pKa = 11.99RR43 pKa = 11.84VISQEE48 pKa = 3.73DD49 pKa = 3.37LKK51 pKa = 11.44LFTEE55 pKa = 4.24NDD57 pKa = 3.38SLRR60 pKa = 11.84ILQYY64 pKa = 10.57IEE66 pKa = 5.91DD67 pKa = 4.04NQNNLQKK74 pKa = 11.02DD75 pKa = 3.67SFEE78 pKa = 4.54IEE80 pKa = 4.07LMFF83 pKa = 5.63

Molecular weight: 9.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZH12|A0A4D5ZH12_9VIRU Phage_rep_org_N domain-containing protein OS=Streptococcus satellite phage Javan203 OX=2558565 GN=JavanS203_0009 PE=4 SV=1
MM1 pKa = 6.55VTVKK5 pKa = 10.36RR6 pKa = 11.84LAIEE10 pKa = 5.67AITKK14 pKa = 9.36PMKK17 pKa = 10.75LKK19 pKa = 10.93GLTKK23 pKa = 10.43AVARR27 pKa = 11.84LDD29 pKa = 4.12GEE31 pKa = 4.08RR32 pKa = 11.84LDD34 pKa = 4.32INLEE38 pKa = 3.77ALFIEE43 pKa = 5.26FEE45 pKa = 4.26SQRR48 pKa = 11.84LEE50 pKa = 3.89LDD52 pKa = 3.79KK53 pKa = 11.19IAGTKK58 pKa = 9.44GGYY61 pKa = 9.59RR62 pKa = 11.84YY63 pKa = 10.0FFLCPRR69 pKa = 11.84CQKK72 pKa = 10.16RR73 pKa = 11.84CRR75 pKa = 11.84VLYY78 pKa = 10.13KK79 pKa = 10.42RR80 pKa = 11.84EE81 pKa = 3.28IAYY84 pKa = 9.4YY85 pKa = 10.69CRR87 pKa = 11.84MCQGIHH93 pKa = 5.97KK94 pKa = 8.33QTLNRR99 pKa = 11.84SKK101 pKa = 9.84TDD103 pKa = 3.25CQYY106 pKa = 11.19YY107 pKa = 9.02WEE109 pKa = 4.76LALKK113 pKa = 9.09EE114 pKa = 4.0VRR116 pKa = 11.84KK117 pKa = 9.48IDD119 pKa = 3.77PYY121 pKa = 9.89FTPKK125 pKa = 10.2RR126 pKa = 11.84GGYY129 pKa = 8.64MFDD132 pKa = 4.25GFPEE136 pKa = 4.16RR137 pKa = 11.84PKK139 pKa = 10.79YY140 pKa = 10.06MRR142 pKa = 11.84INTYY146 pKa = 9.68LKK148 pKa = 9.27HH149 pKa = 5.15YY150 pKa = 10.28RR151 pKa = 11.84RR152 pKa = 11.84FRR154 pKa = 11.84KK155 pKa = 9.77YY156 pKa = 10.2VEE158 pKa = 4.72KK159 pKa = 10.85GDD161 pKa = 4.1SLWLKK166 pKa = 10.6GFF168 pKa = 3.94

Molecular weight: 20.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

1988

39

286

124.3

14.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.332 ± 0.589

0.905 ± 0.267

5.131 ± 0.399

8.048 ± 0.696

4.427 ± 0.685

4.326 ± 0.418

1.408 ± 0.422

6.891 ± 0.333

10.262 ± 0.646

10.463 ± 0.555

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.294

6.087 ± 0.602

2.465 ± 0.214

4.276 ± 0.496

5.131 ± 0.738

4.879 ± 0.472

5.382 ± 0.412

5.131 ± 0.546

1.107 ± 0.204

5.684 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski