Amino acid dipepetide frequency for Microbacterium phage Akoni

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.483AlaAla: 11.483 ± 1.135
0.352AlaCys: 0.352 ± 0.175
5.8AlaAsp: 5.8 ± 0.627
6.21AlaGlu: 6.21 ± 0.77
2.929AlaPhe: 2.929 ± 0.409
6.855AlaGly: 6.855 ± 0.743
1.699AlaHis: 1.699 ± 0.283
3.691AlaIle: 3.691 ± 0.321
2.637AlaLys: 2.637 ± 0.426
8.378AlaLeu: 8.378 ± 0.694
2.637AlaMet: 2.637 ± 0.535
3.867AlaAsn: 3.867 ± 0.551
3.984AlaPro: 3.984 ± 0.573
6.093AlaGln: 6.093 ± 1.002
6.269AlaArg: 6.269 ± 0.523
6.562AlaSer: 6.562 ± 0.721
5.8AlaThr: 5.8 ± 0.548
5.332AlaVal: 5.332 ± 0.741
1.699AlaTrp: 1.699 ± 0.365
2.519AlaTyr: 2.519 ± 0.313
0.0AlaXaa: 0.0 ± 0.0
Cys
0.234CysAla: 0.234 ± 0.127
0.0CysCys: 0.0 ± 0.0
0.059CysAsp: 0.059 ± 0.069
0.41CysGlu: 0.41 ± 0.2
0.234CysPhe: 0.234 ± 0.165
0.41CysGly: 0.41 ± 0.2
0.176CysHis: 0.176 ± 0.11
0.234CysIle: 0.234 ± 0.157
0.176CysLys: 0.176 ± 0.134
0.352CysLeu: 0.352 ± 0.18
0.117CysMet: 0.117 ± 0.106
0.176CysAsn: 0.176 ± 0.116
0.293CysPro: 0.293 ± 0.17
0.0CysGln: 0.0 ± 0.0
0.059CysArg: 0.059 ± 0.063
0.469CysSer: 0.469 ± 0.226
0.176CysThr: 0.176 ± 0.126
0.41CysVal: 0.41 ± 0.208
0.059CysTrp: 0.059 ± 0.069
0.293CysTyr: 0.293 ± 0.146
0.0CysXaa: 0.0 ± 0.0
Asp
6.796AspAla: 6.796 ± 0.795
0.176AspCys: 0.176 ± 0.119
4.453AspAsp: 4.453 ± 0.596
4.804AspGlu: 4.804 ± 0.63
1.933AspPhe: 1.933 ± 0.388
5.507AspGly: 5.507 ± 0.495
0.937AspHis: 0.937 ± 0.221
3.457AspIle: 3.457 ± 0.431
3.34AspLys: 3.34 ± 0.486
4.863AspLeu: 4.863 ± 0.614
1.465AspMet: 1.465 ± 0.269
2.578AspAsn: 2.578 ± 0.419
3.457AspPro: 3.457 ± 0.505
3.398AspGln: 3.398 ± 0.282
3.633AspArg: 3.633 ± 0.413
3.457AspSer: 3.457 ± 0.427
3.633AspThr: 3.633 ± 0.414
4.277AspVal: 4.277 ± 0.561
1.64AspTrp: 1.64 ± 0.27
1.758AspTyr: 1.758 ± 0.343
0.0AspXaa: 0.0 ± 0.0
Glu
5.742GluAla: 5.742 ± 0.566
0.234GluCys: 0.234 ± 0.14
4.101GluAsp: 4.101 ± 0.477
4.394GluGlu: 4.394 ± 0.837
2.402GluPhe: 2.402 ± 0.341
4.746GluGly: 4.746 ± 0.598
1.523GluHis: 1.523 ± 0.437
2.988GluIle: 2.988 ± 0.554
3.34GluLys: 3.34 ± 0.541
6.328GluLeu: 6.328 ± 0.741
2.519GluMet: 2.519 ± 0.334
3.164GluAsn: 3.164 ± 0.391
2.285GluPro: 2.285 ± 0.31
2.402GluGln: 2.402 ± 0.398
4.043GluArg: 4.043 ± 0.623
2.344GluSer: 2.344 ± 0.396
4.453GluThr: 4.453 ± 0.495
4.511GluVal: 4.511 ± 0.506
2.168GluTrp: 2.168 ± 0.305
1.758GluTyr: 1.758 ± 0.345
0.0GluXaa: 0.0 ± 0.0
Phe
2.519PheAla: 2.519 ± 0.343
0.352PheCys: 0.352 ± 0.167
2.871PheAsp: 2.871 ± 0.373
2.637PheGlu: 2.637 ± 0.716
1.875PhePhe: 1.875 ± 0.375
2.402PheGly: 2.402 ± 0.312
0.586PheHis: 0.586 ± 0.225
1.933PheIle: 1.933 ± 0.391
1.172PheLys: 1.172 ± 0.348
1.582PheLeu: 1.582 ± 0.342
0.937PheMet: 0.937 ± 0.338
1.816PheAsn: 1.816 ± 0.272
1.113PhePro: 1.113 ± 0.403
1.406PheGln: 1.406 ± 0.199
2.402PheArg: 2.402 ± 0.375
2.285PheSer: 2.285 ± 0.275
2.519PheThr: 2.519 ± 0.372
2.051PheVal: 2.051 ± 0.356
0.352PheTrp: 0.352 ± 0.141
0.703PheTyr: 0.703 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
7.148GlyAla: 7.148 ± 0.835
0.234GlyCys: 0.234 ± 0.135
4.863GlyAsp: 4.863 ± 0.492
3.867GlyGlu: 3.867 ± 0.517
2.461GlyPhe: 2.461 ± 0.35
7.382GlyGly: 7.382 ± 1.084
1.113GlyHis: 1.113 ± 0.232
4.687GlyIle: 4.687 ± 0.544
3.047GlyLys: 3.047 ± 0.668
6.796GlyLeu: 6.796 ± 1.079
2.109GlyMet: 2.109 ± 0.376
3.281GlyAsn: 3.281 ± 0.6
1.699GlyPro: 1.699 ± 0.247
3.164GlyGln: 3.164 ± 0.374
4.101GlyArg: 4.101 ± 0.681
5.039GlySer: 5.039 ± 0.544
5.449GlyThr: 5.449 ± 0.621
5.332GlyVal: 5.332 ± 0.628
1.582GlyTrp: 1.582 ± 0.213
2.988GlyTyr: 2.988 ± 0.449
0.0GlyXaa: 0.0 ± 0.0
His
1.582HisAla: 1.582 ± 0.269
0.293HisCys: 0.293 ± 0.139
1.465HisAsp: 1.465 ± 0.443
0.879HisGlu: 0.879 ± 0.301
0.352HisPhe: 0.352 ± 0.121
1.64HisGly: 1.64 ± 0.245
0.234HisHis: 0.234 ± 0.108
0.703HisIle: 0.703 ± 0.204
1.348HisLys: 1.348 ± 0.385
1.113HisLeu: 1.113 ± 0.246
0.293HisMet: 0.293 ± 0.155
0.762HisAsn: 0.762 ± 0.2
1.172HisPro: 1.172 ± 0.343
0.293HisGln: 0.293 ± 0.131
1.406HisArg: 1.406 ± 0.231
0.879HisSer: 0.879 ± 0.245
1.113HisThr: 1.113 ± 0.268
1.055HisVal: 1.055 ± 0.262
0.41HisTrp: 0.41 ± 0.148
0.352HisTyr: 0.352 ± 0.131
0.0HisXaa: 0.0 ± 0.0
Ile
4.863IleAla: 4.863 ± 0.536
0.117IleCys: 0.117 ± 0.095
3.222IleAsp: 3.222 ± 0.44
3.047IleGlu: 3.047 ± 0.407
1.406IlePhe: 1.406 ± 0.316
3.105IleGly: 3.105 ± 0.414
0.996IleHis: 0.996 ± 0.217
2.812IleIle: 2.812 ± 0.456
2.871IleLys: 2.871 ± 0.458
3.808IleLeu: 3.808 ± 0.515
1.113IleMet: 1.113 ± 0.237
2.402IleAsn: 2.402 ± 0.279
2.344IlePro: 2.344 ± 0.556
1.816IleGln: 1.816 ± 0.383
2.754IleArg: 2.754 ± 0.371
3.222IleSer: 3.222 ± 0.461
3.105IleThr: 3.105 ± 0.528
3.867IleVal: 3.867 ± 0.643
0.644IleTrp: 0.644 ± 0.145
0.937IleTyr: 0.937 ± 0.186
0.0IleXaa: 0.0 ± 0.0
Lys
4.043LysAla: 4.043 ± 0.551
0.293LysCys: 0.293 ± 0.154
3.164LysAsp: 3.164 ± 0.45
2.285LysGlu: 2.285 ± 0.527
1.348LysPhe: 1.348 ± 0.319
3.105LysGly: 3.105 ± 0.334
0.586LysHis: 0.586 ± 0.25
2.637LysIle: 2.637 ± 0.442
2.285LysLys: 2.285 ± 0.492
4.336LysLeu: 4.336 ± 0.681
0.879LysMet: 0.879 ± 0.221
1.816LysAsn: 1.816 ± 0.297
2.168LysPro: 2.168 ± 0.399
1.523LysGln: 1.523 ± 0.296
2.754LysArg: 2.754 ± 0.644
1.992LysSer: 1.992 ± 0.391
3.457LysThr: 3.457 ± 0.597
2.871LysVal: 2.871 ± 0.444
0.586LysTrp: 0.586 ± 0.245
1.64LysTyr: 1.64 ± 0.422
0.0LysXaa: 0.0 ± 0.0
Leu
7.324LeuAla: 7.324 ± 0.633
0.352LeuCys: 0.352 ± 0.186
4.746LeuAsp: 4.746 ± 0.576
5.8LeuGlu: 5.8 ± 0.654
2.929LeuPhe: 2.929 ± 0.43
5.683LeuGly: 5.683 ± 0.565
1.64LeuHis: 1.64 ± 0.34
3.808LeuIle: 3.808 ± 0.465
3.457LeuLys: 3.457 ± 0.751
6.503LeuLeu: 6.503 ± 0.669
2.285LeuMet: 2.285 ± 0.364
4.336LeuAsn: 4.336 ± 0.559
3.925LeuPro: 3.925 ± 0.532
4.277LeuGln: 4.277 ± 0.709
5.859LeuArg: 5.859 ± 0.471
5.039LeuSer: 5.039 ± 0.542
6.093LeuThr: 6.093 ± 0.745
4.863LeuVal: 4.863 ± 0.44
1.172LeuTrp: 1.172 ± 0.192
2.226LeuTyr: 2.226 ± 0.344
0.0LeuXaa: 0.0 ± 0.0
Met
2.519MetAla: 2.519 ± 0.36
0.059MetCys: 0.059 ± 0.076
1.816MetAsp: 1.816 ± 0.283
1.406MetGlu: 1.406 ± 0.286
1.23MetPhe: 1.23 ± 0.269
1.23MetGly: 1.23 ± 0.321
0.469MetHis: 0.469 ± 0.16
0.879MetIle: 0.879 ± 0.238
1.23MetLys: 1.23 ± 0.218
1.699MetLeu: 1.699 ± 0.307
0.82MetMet: 0.82 ± 0.238
0.937MetAsn: 0.937 ± 0.223
1.758MetPro: 1.758 ± 0.3
1.465MetGln: 1.465 ± 0.299
1.523MetArg: 1.523 ± 0.294
1.875MetSer: 1.875 ± 0.408
1.992MetThr: 1.992 ± 0.343
1.113MetVal: 1.113 ± 0.252
0.586MetTrp: 0.586 ± 0.173
0.644MetTyr: 0.644 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
4.101AsnAla: 4.101 ± 0.554
0.117AsnCys: 0.117 ± 0.09
3.222AsnAsp: 3.222 ± 0.377
2.637AsnGlu: 2.637 ± 0.279
1.113AsnPhe: 1.113 ± 0.214
3.867AsnGly: 3.867 ± 0.496
0.469AsnHis: 0.469 ± 0.224
2.168AsnIle: 2.168 ± 0.308
1.933AsnLys: 1.933 ± 0.28
3.105AsnLeu: 3.105 ± 0.451
0.879AsnMet: 0.879 ± 0.216
2.461AsnAsn: 2.461 ± 0.424
3.515AsnPro: 3.515 ± 0.549
2.226AsnGln: 2.226 ± 0.251
2.285AsnArg: 2.285 ± 0.461
2.929AsnSer: 2.929 ± 0.407
3.222AsnThr: 3.222 ± 0.534
3.633AsnVal: 3.633 ± 0.372
1.172AsnTrp: 1.172 ± 0.333
1.758AsnTyr: 1.758 ± 0.342
0.0AsnXaa: 0.0 ± 0.0
Pro
4.101ProAla: 4.101 ± 0.737
0.176ProCys: 0.176 ± 0.104
3.281ProAsp: 3.281 ± 0.459
4.687ProGlu: 4.687 ± 0.44
1.289ProPhe: 1.289 ± 0.316
3.75ProGly: 3.75 ± 0.45
0.644ProHis: 0.644 ± 0.195
1.816ProIle: 1.816 ± 0.426
2.285ProLys: 2.285 ± 0.437
2.754ProLeu: 2.754 ± 0.279
0.996ProMet: 0.996 ± 0.218
2.402ProAsn: 2.402 ± 0.401
1.582ProPro: 1.582 ± 0.394
1.758ProGln: 1.758 ± 0.268
2.168ProArg: 2.168 ± 0.507
3.34ProSer: 3.34 ± 0.442
4.043ProThr: 4.043 ± 0.737
3.398ProVal: 3.398 ± 0.332
1.23ProTrp: 1.23 ± 0.267
1.582ProTyr: 1.582 ± 0.217
0.0ProXaa: 0.0 ± 0.0
Gln
5.625GlnAla: 5.625 ± 0.507
0.293GlnCys: 0.293 ± 0.144
3.105GlnAsp: 3.105 ± 0.506
2.988GlnGlu: 2.988 ± 0.448
1.523GlnPhe: 1.523 ± 0.258
2.461GlnGly: 2.461 ± 0.305
0.762GlnHis: 0.762 ± 0.237
2.344GlnIle: 2.344 ± 0.34
1.523GlnLys: 1.523 ± 0.293
4.336GlnLeu: 4.336 ± 0.363
1.406GlnMet: 1.406 ± 0.38
2.226GlnAsn: 2.226 ± 0.33
1.699GlnPro: 1.699 ± 0.283
2.109GlnGln: 2.109 ± 0.462
2.578GlnArg: 2.578 ± 0.346
2.109GlnSer: 2.109 ± 0.353
3.75GlnThr: 3.75 ± 0.929
2.109GlnVal: 2.109 ± 0.306
0.762GlnTrp: 0.762 ± 0.205
1.582GlnTyr: 1.582 ± 0.289
0.0GlnXaa: 0.0 ± 0.0
Arg
5.156ArgAla: 5.156 ± 0.704
0.176ArgCys: 0.176 ± 0.113
3.574ArgAsp: 3.574 ± 0.421
4.16ArgGlu: 4.16 ± 0.556
1.992ArgPhe: 1.992 ± 0.394
4.921ArgGly: 4.921 ± 0.714
1.055ArgHis: 1.055 ± 0.315
2.988ArgIle: 2.988 ± 0.432
2.168ArgLys: 2.168 ± 0.408
5.214ArgLeu: 5.214 ± 0.504
1.875ArgMet: 1.875 ± 0.313
3.164ArgAsn: 3.164 ± 0.35
2.344ArgPro: 2.344 ± 0.426
2.988ArgGln: 2.988 ± 0.465
4.218ArgArg: 4.218 ± 0.744
3.574ArgSer: 3.574 ± 0.598
3.398ArgThr: 3.398 ± 0.552
3.75ArgVal: 3.75 ± 0.468
0.937ArgTrp: 0.937 ± 0.256
1.758ArgTyr: 1.758 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
5.625SerAla: 5.625 ± 0.68
0.293SerCys: 0.293 ± 0.141
3.222SerAsp: 3.222 ± 0.512
4.16SerGlu: 4.16 ± 0.439
2.578SerPhe: 2.578 ± 0.374
4.804SerGly: 4.804 ± 0.629
1.113SerHis: 1.113 ± 0.252
3.398SerIle: 3.398 ± 0.531
2.168SerLys: 2.168 ± 0.24
4.98SerLeu: 4.98 ± 0.542
1.582SerMet: 1.582 ± 0.265
2.637SerAsn: 2.637 ± 0.483
3.925SerPro: 3.925 ± 0.408
2.519SerGln: 2.519 ± 0.45
2.871SerArg: 2.871 ± 0.434
2.929SerSer: 2.929 ± 0.438
4.453SerThr: 4.453 ± 0.745
3.867SerVal: 3.867 ± 0.495
0.703SerTrp: 0.703 ± 0.261
1.699SerTyr: 1.699 ± 0.383
0.0SerXaa: 0.0 ± 0.0
Thr
6.914ThrAla: 6.914 ± 0.609
0.234ThrCys: 0.234 ± 0.141
4.043ThrAsp: 4.043 ± 0.567
4.453ThrGlu: 4.453 ± 0.492
2.754ThrPhe: 2.754 ± 0.368
6.738ThrGly: 6.738 ± 1.01
0.879ThrHis: 0.879 ± 0.352
2.519ThrIle: 2.519 ± 0.426
2.637ThrLys: 2.637 ± 0.415
5.976ThrLeu: 5.976 ± 0.709
0.82ThrMet: 0.82 ± 0.244
3.574ThrAsn: 3.574 ± 0.367
4.277ThrPro: 4.277 ± 0.627
2.929ThrGln: 2.929 ± 0.504
4.394ThrArg: 4.394 ± 0.513
4.218ThrSer: 4.218 ± 0.576
5.156ThrThr: 5.156 ± 0.509
4.57ThrVal: 4.57 ± 0.549
0.82ThrTrp: 0.82 ± 0.193
1.992ThrTyr: 1.992 ± 0.263
0.0ThrXaa: 0.0 ± 0.0
Val
6.269ValAla: 6.269 ± 0.516
0.293ValCys: 0.293 ± 0.161
5.097ValAsp: 5.097 ± 0.604
3.457ValGlu: 3.457 ± 0.549
1.523ValPhe: 1.523 ± 0.348
4.277ValGly: 4.277 ± 0.393
1.523ValHis: 1.523 ± 0.318
2.871ValIle: 2.871 ± 0.355
2.988ValLys: 2.988 ± 0.461
5.683ValLeu: 5.683 ± 0.705
1.64ValMet: 1.64 ± 0.296
2.812ValAsn: 2.812 ± 0.468
4.16ValPro: 4.16 ± 0.556
3.105ValGln: 3.105 ± 0.447
3.515ValArg: 3.515 ± 0.568
3.984ValSer: 3.984 ± 0.519
4.863ValThr: 4.863 ± 0.426
4.511ValVal: 4.511 ± 0.741
1.289ValTrp: 1.289 ± 0.324
1.816ValTyr: 1.816 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
1.172TrpAla: 1.172 ± 0.249
0.117TrpCys: 0.117 ± 0.084
1.348TrpAsp: 1.348 ± 0.269
1.172TrpGlu: 1.172 ± 0.325
0.586TrpPhe: 0.586 ± 0.214
1.289TrpGly: 1.289 ± 0.321
0.293TrpHis: 0.293 ± 0.124
1.23TrpIle: 1.23 ± 0.357
1.23TrpLys: 1.23 ± 0.315
1.64TrpLeu: 1.64 ± 0.322
0.234TrpMet: 0.234 ± 0.13
0.762TrpAsn: 0.762 ± 0.205
0.527TrpPro: 0.527 ± 0.154
0.762TrpGln: 0.762 ± 0.211
0.937TrpArg: 0.937 ± 0.228
1.406TrpSer: 1.406 ± 0.413
1.523TrpThr: 1.523 ± 0.239
1.172TrpVal: 1.172 ± 0.245
0.469TrpTrp: 0.469 ± 0.183
0.937TrpTyr: 0.937 ± 0.274
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.582TyrAla: 1.582 ± 0.329
0.176TyrCys: 0.176 ± 0.102
2.168TyrAsp: 2.168 ± 0.345
1.992TyrGlu: 1.992 ± 0.388
1.055TyrPhe: 1.055 ± 0.269
2.168TyrGly: 2.168 ± 0.389
0.644TyrHis: 0.644 ± 0.238
1.289TyrIle: 1.289 ± 0.22
1.933TyrLys: 1.933 ± 0.4
2.871TyrLeu: 2.871 ± 0.382
0.586TyrMet: 0.586 ± 0.206
1.64TyrAsn: 1.64 ± 0.257
1.055TyrPro: 1.055 ± 0.388
1.055TyrGln: 1.055 ± 0.263
1.64TyrArg: 1.64 ± 0.278
1.875TyrSer: 1.875 ± 0.374
1.699TyrThr: 1.699 ± 0.363
2.988TyrVal: 2.988 ± 0.595
0.527TyrTrp: 0.527 ± 0.165
1.23TyrTyr: 1.23 ± 0.295
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (17069 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski