Vibrio phage KVP40 (isolate Vibrio parahaemolyticus/Japan/Matsuzaki/1991) (KVP40) (Bacteriophage KVP40)
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 380 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6WI50|Q6WI50_BPKVM Uncharacterized protein OS=Vibrio phage KVP40 (isolate Vibrio parahaemolyticus/Japan/Matsuzaki/1991) OX=1283340 GN=KVP40.0102 PE=4 SV=1
MM1 pKa = 7.6 IVLQHH6 pKa = 5.85 EE7 pKa = 4.25 TDD9 pKa = 3.84 GYY11 pKa = 10.16 IVQIGCDD18 pKa = 3.35 ICCTPDD24 pKa = 2.95 IDD26 pKa = 3.94 KK27 pKa = 11.35 ASMFEE32 pKa = 4.36 TVGVAQEE39 pKa = 5.99 FKK41 pKa = 10.83 DD42 pKa = 5.27 QWDD45 pKa = 3.41 LWDD48 pKa = 3.46 WEE50 pKa = 4.2 VRR52 pKa = 11.84 EE53 pKa = 4.16 VGLL56 pKa = 4.91
Molecular weight: 6.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.706
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|Q6WHD7|Q6WHD7_BPKVM Uncharacterized protein OS=Vibrio phage KVP40 (isolate Vibrio parahaemolyticus/Japan/Matsuzaki/1991) OX=1283340 GN=KVP40.0368 PE=4 SV=1
MM1 pKa = 7.61 IIKK4 pKa = 10.15 KK5 pKa = 10.17 RR6 pKa = 11.84 ILRR9 pKa = 11.84 FFCIYY14 pKa = 10.37 WLTTTPKK21 pKa = 10.32 SRR23 pKa = 11.84 NISINEE29 pKa = 3.98 TNRR32 pKa = 11.84 PQQEE36 pKa = 4.15 RR37 pKa = 11.84 ILL39 pKa = 3.97
Molecular weight: 4.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.304
IPC2_protein 9.882
IPC_protein 10.906
Toseland 11.111
ProMoST 11.111
Dawson 11.155
Bjellqvist 10.95
Wikipedia 11.447
Rodwell 11.286
Grimsley 11.199
Solomon 11.418
Lehninger 11.359
Nozaki 11.096
DTASelect 10.95
Thurlkill 11.096
EMBOSS 11.55
Sillero 11.111
Patrickios 11.096
IPC_peptide 11.433
IPC2_peptide 10.218
IPC2.peptide.svr19 8.621
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
380
0
380
73563
36
1256
193.6
22.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.029 ± 0.138
1.259 ± 0.065
6.729 ± 0.106
7.608 ± 0.144
4.289 ± 0.095
5.894 ± 0.14
2.308 ± 0.086
6.666 ± 0.109
6.651 ± 0.184
7.54 ± 0.116
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.856 ± 0.082
5.1 ± 0.097
3.144 ± 0.087
3.413 ± 0.076
4.99 ± 0.099
6.063 ± 0.139
6.033 ± 0.172
6.862 ± 0.102
1.359 ± 0.062
4.203 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here