Myroides odoratus DSM 2801
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3687 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H1Z978|H1Z978_MYROD Transporter hydrophobe/amphiphile efflux-1 (HAE1) family OS=Myroides odoratus DSM 2801 OX=929704 GN=Myrod_2783 PE=3 SV=1
MM1 pKa = 7.49 TNNFEE6 pKa = 4.36 EE7 pKa = 4.86 IINRR11 pKa = 11.84 TEE13 pKa = 3.82 VQEE16 pKa = 4.11 VLNQYY21 pKa = 8.08 ITEE24 pKa = 4.22 NGGNIHH30 pKa = 6.63 YY31 pKa = 10.48 DD32 pKa = 3.3 GGSFTYY38 pKa = 10.63 LDD40 pKa = 3.86 EE41 pKa = 4.87 NGNTVTMDD49 pKa = 3.3 MTTIIQGSEE58 pKa = 4.0 TVTTLVKK65 pKa = 10.84 DD66 pKa = 3.51 PLNNGKK72 pKa = 10.23 YY73 pKa = 9.93 IYY75 pKa = 9.73 TNEE78 pKa = 4.07 AGVEE82 pKa = 4.18 VVIDD86 pKa = 3.51 VTTEE90 pKa = 3.51 VTNNFEE96 pKa = 4.27 EE97 pKa = 4.81 IINRR101 pKa = 11.84 TEE103 pKa = 3.82 VQEE106 pKa = 4.11 VLNQYY111 pKa = 8.08 ITEE114 pKa = 4.22 NGGNIHH120 pKa = 6.63 YY121 pKa = 10.48 DD122 pKa = 3.3 GGSFTYY128 pKa = 10.63 LDD130 pKa = 3.86 EE131 pKa = 4.87 NGNTVTMDD139 pKa = 3.02 MTTIVQGSEE148 pKa = 3.94 TVTTIVKK155 pKa = 10.13 DD156 pKa = 3.46 PSGTYY161 pKa = 9.59 TYY163 pKa = 10.57 TNEE166 pKa = 4.03 AGVAMVIDD174 pKa = 3.89 VQADD178 pKa = 3.49 VVNNFEE184 pKa = 4.36 EE185 pKa = 4.91 IINKK189 pKa = 8.56 TEE191 pKa = 3.94 VQEE194 pKa = 5.68 LLTQVINNNGGNVHH208 pKa = 6.46 FDD210 pKa = 3.22 EE211 pKa = 6.44 GGFTYY216 pKa = 10.37 TDD218 pKa = 3.47 EE219 pKa = 4.69 NGASQTVDD227 pKa = 2.82 LTTIVQGSEE236 pKa = 3.94 TVTTIVKK243 pKa = 10.13 DD244 pKa = 3.46 PSGTYY249 pKa = 9.59 TYY251 pKa = 10.57 TNEE254 pKa = 4.03 AGVAMVIDD262 pKa = 3.89 VQADD266 pKa = 3.49 VVNNFEE272 pKa = 4.36 EE273 pKa = 4.91 IINKK277 pKa = 8.56 TEE279 pKa = 3.94 VQEE282 pKa = 5.68 LLTQVINNNGGNVHH296 pKa = 6.46 FDD298 pKa = 3.22 EE299 pKa = 6.44 GGFTYY304 pKa = 10.37 TDD306 pKa = 3.47 EE307 pKa = 4.69 NGASQTVDD315 pKa = 2.82 LTTIVQGSEE324 pKa = 3.94 TVTTIVKK331 pKa = 10.13 DD332 pKa = 3.46 PSGTYY337 pKa = 9.59 TYY339 pKa = 10.57 TNEE342 pKa = 4.03 AGVAMVIDD350 pKa = 3.89 VQADD354 pKa = 3.49 VVNNFEE360 pKa = 4.36 EE361 pKa = 4.91 IINKK365 pKa = 8.56 TEE367 pKa = 3.94 VQEE370 pKa = 5.68 LLTQVINNNGGNVHH384 pKa = 6.46 FDD386 pKa = 3.22 EE387 pKa = 6.44 GGFTYY392 pKa = 10.37 TDD394 pKa = 3.47 EE395 pKa = 4.69 NGASQTVDD403 pKa = 2.82 LTTIVQGSEE412 pKa = 3.94 TVTTIVKK419 pKa = 10.13 DD420 pKa = 3.46 PSGTYY425 pKa = 9.59 TYY427 pKa = 10.57 TNEE430 pKa = 3.8 AGVAVVIDD438 pKa = 3.68 VQADD442 pKa = 3.52 VVNNFEE448 pKa = 4.36 EE449 pKa = 4.91 IINKK453 pKa = 8.56 TEE455 pKa = 3.94 VQEE458 pKa = 5.68 LLTQVINNNGGNVHH472 pKa = 6.46 FDD474 pKa = 3.22 EE475 pKa = 6.44 GGFTYY480 pKa = 10.37 TDD482 pKa = 3.47 EE483 pKa = 4.69 NGASQTVDD491 pKa = 2.82 LTTIVQGSEE500 pKa = 3.94 TVTTIVKK507 pKa = 10.13 DD508 pKa = 3.46 PSGTYY513 pKa = 9.59 TYY515 pKa = 10.57 TNEE518 pKa = 4.03 AGVAMVIDD526 pKa = 3.89 VQADD530 pKa = 3.49 VVNNFEE536 pKa = 4.36 EE537 pKa = 4.91 IINKK541 pKa = 8.56 TEE543 pKa = 3.94 VQEE546 pKa = 5.36 LLTQVVNNVGGNMTYY561 pKa = 10.83 DD562 pKa = 3.47 GTGFTYY568 pKa = 10.63 TDD570 pKa = 3.61 GNGDD574 pKa = 3.81 TVAMDD579 pKa = 3.48 MSTFVQGNEE588 pKa = 3.87 TTTTLVKK595 pKa = 10.57 DD596 pKa = 3.57 ASDD599 pKa = 3.52 NGKK602 pKa = 8.5 YY603 pKa = 9.22 TYY605 pKa = 10.52 TNEE608 pKa = 4.02 TNTTVEE614 pKa = 4.15 LDD616 pKa = 3.23 IPTDD620 pKa = 3.53 VINNFEE626 pKa = 4.63 TIVADD631 pKa = 3.95 ANVGNLLQQLVVKK644 pKa = 10.73 SNTLVEE650 pKa = 4.15 TAVNYY655 pKa = 10.06 VVLDD659 pKa = 3.45 VDD661 pKa = 3.49 TTIIVDD667 pKa = 3.87 AEE669 pKa = 4.16 AADD672 pKa = 3.85 VTITLPAATSEE683 pKa = 4.17 NKK685 pKa = 10.17 GRR687 pKa = 11.84 MITIRR692 pKa = 11.84 KK693 pKa = 8.81 IDD695 pKa = 3.63 EE696 pKa = 3.97 SDD698 pKa = 3.26 NAVNFSQQIKK708 pKa = 7.96 TSKK711 pKa = 9.75 TEE713 pKa = 3.84 GFTSINFGITVNIQSNGSVWYY734 pKa = 8.63 MINN737 pKa = 3.18
Molecular weight: 80.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.465
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.541
Rodwell 3.503
Grimsley 3.376
Solomon 3.643
Lehninger 3.592
Nozaki 3.745
DTASelect 3.935
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.783
Patrickios 0.871
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|H1Z845|H1Z845_MYROD Quinolinate phosphoribosyltransferase [decarboxylating] OS=Myroides odoratus DSM 2801 OX=929704 GN=Myrod_0998 PE=3 SV=1
MM1 pKa = 7.31 NRR3 pKa = 11.84 FSLSFLRR10 pKa = 11.84 SIAFLFLVVGMILMVQNIDD29 pKa = 3.23 QFLYY33 pKa = 10.53 KK34 pKa = 9.1 QTLEE38 pKa = 3.92 IGSLAFALACITISILGQRR57 pKa = 11.84 KK58 pKa = 8.7 LKK60 pKa = 10.43 KK61 pKa = 8.82 STRR64 pKa = 11.84 ISNLL68 pKa = 3.06
Molecular weight: 7.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.736
IPC_protein 10.248
Toseland 10.818
ProMoST 10.394
Dawson 10.891
Bjellqvist 10.54
Wikipedia 11.052
Rodwell 11.286
Grimsley 10.921
Solomon 10.994
Lehninger 10.979
Nozaki 10.804
DTASelect 10.54
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.818
Patrickios 11.096
IPC_peptide 11.008
IPC2_peptide 9.472
IPC2.peptide.svr19 8.572
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3687
0
3687
1212748
30
2726
328.9
37.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.721 ± 0.043
0.761 ± 0.011
5.268 ± 0.03
6.653 ± 0.044
5.061 ± 0.03
6.284 ± 0.042
1.867 ± 0.019
7.546 ± 0.04
7.107 ± 0.042
9.558 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.257 ± 0.02
5.549 ± 0.046
3.324 ± 0.023
4.283 ± 0.031
3.477 ± 0.027
5.952 ± 0.029
6.158 ± 0.051
6.68 ± 0.036
1.042 ± 0.013
4.454 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here