Northern cereal mosaic virus (NCMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9JGU1|NCAP_NCMV Nucleoprotein OS=Northern cereal mosaic virus OX=1985704 GN=N PE=3 SV=1
MM1 pKa = 7.94DD2 pKa = 4.9KK3 pKa = 10.38KK4 pKa = 10.99ASGISGEE11 pKa = 4.06NALFGDD17 pKa = 3.99VPNDD21 pKa = 3.63VVGTTYY27 pKa = 11.37EE28 pKa = 4.05MGLDD32 pKa = 4.28GIFDD36 pKa = 4.37DD37 pKa = 5.89GEE39 pKa = 4.26TDD41 pKa = 4.03VIEE44 pKa = 4.73SPADD48 pKa = 3.48AEE50 pKa = 4.37EE51 pKa = 4.52HH52 pKa = 4.93VTTDD56 pKa = 2.77IVADD60 pKa = 3.98EE61 pKa = 4.59GDD63 pKa = 3.65NLITKK68 pKa = 9.45VDD70 pKa = 3.72DD71 pKa = 3.84MIGYY75 pKa = 9.26LKK77 pKa = 10.51RR78 pKa = 11.84EE79 pKa = 4.29CQDD82 pKa = 2.58HH83 pKa = 6.5GIAVRR88 pKa = 11.84KK89 pKa = 9.12EE90 pKa = 3.8WVNVLTRR97 pKa = 11.84KK98 pKa = 9.27FHH100 pKa = 5.47MSKK103 pKa = 10.45KK104 pKa = 9.83IYY106 pKa = 10.2ASHH109 pKa = 7.52LDD111 pKa = 3.7FFLLGVMGEE120 pKa = 3.88RR121 pKa = 11.84HH122 pKa = 5.04VAVEE126 pKa = 4.53KK127 pKa = 10.77DD128 pKa = 3.85FKK130 pKa = 10.57DD131 pKa = 3.37TAVRR135 pKa = 11.84LSDD138 pKa = 4.01EE139 pKa = 4.34VNKK142 pKa = 10.32MSGISKK148 pKa = 10.05KK149 pKa = 10.63VADD152 pKa = 4.24NEE154 pKa = 4.0TRR156 pKa = 11.84MIKK159 pKa = 10.39EE160 pKa = 4.32LDD162 pKa = 3.38AKK164 pKa = 8.94MKK166 pKa = 10.68EE167 pKa = 3.95MGSLIGKK174 pKa = 9.26FNGVLNEE181 pKa = 4.41FKK183 pKa = 11.03GSMAVSSRR191 pKa = 11.84PASVASWALDD201 pKa = 3.21QTTEE205 pKa = 3.69TSRR208 pKa = 11.84EE209 pKa = 3.68KK210 pKa = 10.94NYY212 pKa = 11.11NEE214 pKa = 3.82FLKK217 pKa = 11.08KK218 pKa = 10.56LGFQDD223 pKa = 3.6HH224 pKa = 6.91HH225 pKa = 7.04IKK227 pKa = 10.53SPLMKK232 pKa = 9.7KK233 pKa = 8.11CTVMITDD240 pKa = 3.52EE241 pKa = 4.43MYY243 pKa = 11.23DD244 pKa = 3.42EE245 pKa = 4.93VMLEE249 pKa = 4.05NTSEE253 pKa = 4.06DD254 pKa = 3.25TLGIYY259 pKa = 9.97KK260 pKa = 9.71EE261 pKa = 4.01QIITYY266 pKa = 8.09GQSIQKK272 pKa = 10.18KK273 pKa = 6.86VEE275 pKa = 3.84KK276 pKa = 9.74PIKK279 pKa = 9.33KK280 pKa = 10.16DD281 pKa = 3.25PYY283 pKa = 11.25ADD285 pKa = 3.64FF286 pKa = 5.14

Molecular weight: 32.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9JGT6|VP6_NCMV Protein 6 OS=Northern cereal mosaic virus OX=1985704 GN=6 PE=4 SV=1
MM1 pKa = 6.88ATEE4 pKa = 3.98VSTRR8 pKa = 11.84FIFIKK13 pKa = 10.04IDD15 pKa = 3.45ALSTLHH21 pKa = 7.17FIDD24 pKa = 3.83EE25 pKa = 4.58GGALSMNGDD34 pKa = 3.95SIEE37 pKa = 3.9FLIGKK42 pKa = 9.77SSLDD46 pKa = 3.13KK47 pKa = 11.29GLLKK51 pKa = 10.56RR52 pKa = 11.84ATKK55 pKa = 10.61LFTWLFSPEE64 pKa = 3.5IAKK67 pKa = 9.39QHH69 pKa = 5.81LSITRR74 pKa = 11.84STEE77 pKa = 3.83SNVYY81 pKa = 7.85MTDD84 pKa = 2.82ATRR87 pKa = 11.84YY88 pKa = 9.19KK89 pKa = 10.8YY90 pKa = 10.28IFPEE94 pKa = 4.09YY95 pKa = 10.73LLVRR99 pKa = 11.84YY100 pKa = 9.75VGTKK104 pKa = 9.88FPDD107 pKa = 3.28MKK109 pKa = 10.34IVADD113 pKa = 3.57ATFKK117 pKa = 10.77RR118 pKa = 11.84RR119 pKa = 11.84NPKK122 pKa = 10.22GDD124 pKa = 3.75VIGMLDD130 pKa = 3.64LSFKK134 pKa = 8.96EE135 pKa = 4.09VRR137 pKa = 11.84VKK139 pKa = 10.47EE140 pKa = 4.14VSEE143 pKa = 4.28TKK145 pKa = 10.42AIEE148 pKa = 4.19LRR150 pKa = 11.84DD151 pKa = 3.99SNPGYY156 pKa = 10.7LLSTTYY162 pKa = 10.44RR163 pKa = 11.84EE164 pKa = 4.43SEE166 pKa = 4.28SLPVVSKK173 pKa = 10.52PP174 pKa = 3.48

Molecular weight: 19.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1907

114

483

238.4

26.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.611 ± 0.728

1.888 ± 0.611

5.716 ± 0.817

6.502 ± 0.448

4.038 ± 0.244

6.45 ± 0.372

1.468 ± 0.369

7.184 ± 0.732

7.446 ± 0.664

8.233 ± 0.678

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.937 ± 0.498

3.671 ± 0.373

3.776 ± 0.506

2.832 ± 0.592

4.615 ± 0.622

9.229 ± 0.809

6.24 ± 0.275

7.918 ± 0.72

1.206 ± 0.27

3.041 ± 0.448
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski