Oryctes rhinoceros nudivirus (isolate Oryctes rhinoceros/Malaysia/Ma07/2007) (OrNV)
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 138 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7SVG0|B7SVG0_ORNVM Uncharacterized protein OS=Oryctes rhinoceros nudivirus (isolate Oryctes rhinoceros/Malaysia/Ma07/2007) OX=1046081 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 5.6 EE3 pKa = 4.57 YY4 pKa = 11.53 DD5 pKa = 3.63 EE6 pKa = 6.0 CDD8 pKa = 3.39 VKK10 pKa = 11.23 QPDD13 pKa = 5.15 DD14 pKa = 3.77 MDD16 pKa = 4.2 EE17 pKa = 4.64 DD18 pKa = 4.44 EE19 pKa = 4.74 PTIDD23 pKa = 5.53 DD24 pKa = 4.49 DD25 pKa = 5.24 EE26 pKa = 6.32 EE27 pKa = 5.8 DD28 pKa = 3.56 VDD30 pKa = 4.63 EE31 pKa = 5.58 PISDD35 pKa = 3.82 VVVEE39 pKa = 4.35 YY40 pKa = 11.19 GDD42 pKa = 5.77 DD43 pKa = 3.73 EE44 pKa = 7.09 DD45 pKa = 6.78 DD46 pKa = 5.45 DD47 pKa = 5.39 VVDD50 pKa = 5.63 DD51 pKa = 6.56 DD52 pKa = 5.15 EE53 pKa = 6.26 AEE55 pKa = 4.49 CDD57 pKa = 4.87 DD58 pKa = 4.68 EE59 pKa = 5.54 VCVPDD64 pKa = 4.56 GDD66 pKa = 4.43 EE67 pKa = 4.09 PCKK70 pKa = 10.48 FDD72 pKa = 6.84 DD73 pKa = 3.71 YY74 pKa = 11.64 TLVNNCEE81 pKa = 4.17 STNMVDD87 pKa = 2.78 VWQISTDD94 pKa = 3.79 FTRR97 pKa = 11.84 NDD99 pKa = 4.28 LLTKK103 pKa = 10.33 AKK105 pKa = 10.25 PGTWLYY111 pKa = 11.62 NKK113 pKa = 9.33 ILHH116 pKa = 5.52 STPMLASSILTSHH129 pKa = 6.78 LNVLSVHH136 pKa = 5.83 GVYY139 pKa = 10.54 LAPGNVTASTFYY151 pKa = 10.71 YY152 pKa = 10.0 KK153 pKa = 10.58 KK154 pKa = 10.51 KK155 pKa = 10.76
Molecular weight: 17.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.554
IPC_protein 3.592
Toseland 3.363
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.554
Rodwell 3.414
Grimsley 3.261
Solomon 3.579
Lehninger 3.541
Nozaki 3.706
DTASelect 3.973
Thurlkill 3.427
EMBOSS 3.554
Sillero 3.719
Patrickios 0.807
IPC_peptide 3.579
IPC2_peptide 3.694
IPC2.peptide.svr19 3.669
Protein with the highest isoelectric point:
>tr|B7SV44|B7SV44_ORNVM Guanylate kinase-like protein OS=Oryctes rhinoceros nudivirus (isolate Oryctes rhinoceros/Malaysia/Ma07/2007) OX=1046081 PE=4 SV=1
MM1 pKa = 7.23 NVSITNIQEE10 pKa = 4.62 FINSIHH16 pKa = 7.03 DD17 pKa = 3.96 FIIEE21 pKa = 4.04 TFEE24 pKa = 4.1 IIPRR28 pKa = 11.84 DD29 pKa = 3.9 FEE31 pKa = 4.44 TKK33 pKa = 10.38 LLVGNRR39 pKa = 11.84 SLVWHH44 pKa = 6.73 SDD46 pKa = 2.84 VGKK49 pKa = 10.06 IEE51 pKa = 4.9 EE52 pKa = 4.72 FICPKK57 pKa = 9.89 FKK59 pKa = 10.54 HH60 pKa = 5.81 YY61 pKa = 11.08 LHH63 pKa = 6.32 VHH65 pKa = 6.03 EE66 pKa = 5.16 KK67 pKa = 10.85 HH68 pKa = 6.44 NADD71 pKa = 3.77 EE72 pKa = 5.12 NLTICCNEE80 pKa = 3.48 VDD82 pKa = 4.95 IIALKK87 pKa = 10.27 YY88 pKa = 10.91 LNDD91 pKa = 4.34 SIFGTFKK98 pKa = 11.15 NKK100 pKa = 9.98 IISSLHH106 pKa = 4.99 SWLPAATYY114 pKa = 7.77 QHH116 pKa = 5.47 VRR118 pKa = 11.84 VVVRR122 pKa = 11.84 SNTTYY127 pKa = 9.76 INSYY131 pKa = 10.23 FEE133 pKa = 3.96 PSYY136 pKa = 11.06 LFNLLHH142 pKa = 6.55 YY143 pKa = 9.17 IDD145 pKa = 4.62 HH146 pKa = 6.51 SRR148 pKa = 11.84 DD149 pKa = 3.17 IIYY152 pKa = 8.99 TYY154 pKa = 9.46 YY155 pKa = 10.41 HH156 pKa = 6.59 SNIVLYY162 pKa = 10.33 EE163 pKa = 4.08 SYY165 pKa = 11.06 NLEE168 pKa = 4.28 GINNKK173 pKa = 10.04 RR174 pKa = 11.84 IMVRR178 pKa = 11.84 ARR180 pKa = 11.84 SRR182 pKa = 11.84 SPGRR186 pKa = 11.84 AKK188 pKa = 10.48 SPSRR192 pKa = 11.84 ARR194 pKa = 11.84 SGRR197 pKa = 11.84 ARR199 pKa = 11.84 SPAKK203 pKa = 9.75 ASKK206 pKa = 9.53 RR207 pKa = 11.84 QRR209 pKa = 11.84 SRR211 pKa = 11.84 SRR213 pKa = 11.84 SRR215 pKa = 11.84 SRR217 pKa = 11.84 SRR219 pKa = 11.84 SASRR223 pKa = 11.84 TARR226 pKa = 11.84 RR227 pKa = 11.84 ASSPRR232 pKa = 11.84 RR233 pKa = 11.84 QSSTTSRR240 pKa = 11.84 SRR242 pKa = 11.84 RR243 pKa = 3.56
Molecular weight: 28.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.765
IPC_protein 10.555
Toseland 10.423
ProMoST 10.277
Dawson 10.613
Bjellqvist 10.35
Wikipedia 10.833
Rodwell 10.804
Grimsley 10.687
Solomon 10.672
Lehninger 10.628
Nozaki 10.423
DTASelect 10.335
Thurlkill 10.467
EMBOSS 10.833
Sillero 10.526
Patrickios 10.423
IPC_peptide 10.672
IPC2_peptide 9.341
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
138
0
138
37445
50
1280
271.3
30.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.186 ± 0.144
2.201 ± 0.11
6.391 ± 0.222
5.053 ± 0.15
4.439 ± 0.151
3.629 ± 0.141
2.171 ± 0.103
8.108 ± 0.134
6.401 ± 0.174
8.685 ± 0.19
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.615 ± 0.095
6.134 ± 0.128
4.326 ± 0.144
3.328 ± 0.149
4.473 ± 0.158
8.367 ± 0.19
6.842 ± 0.225
5.958 ± 0.146
0.708 ± 0.051
4.986 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here